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Dichlorodifluoromethane (CAS No. 75-71-8). Profile from Hazardous Substances Data Bank


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DICHLORODIFLUOROMETHANE
CASRN: 75-71-8
For other data, click on the Table of Contents

Human Health Effects:

Human Toxicity Excerpts:

... FATAL CASE OF BRONCHOPNEUMONIA /REPORTED/ IN MAN WHO PUNCTURED FREEZING COIL OF REFRIGERATOR CONTAINING FC-12. IT IS PROBABLE THAT HE ASPIRATED COLD CONCENTRATED VAPOR OR LIQ, OR WAS EXPOSED TO DEGRADATION PRODUCTS OF REFRIGERANT CMPD.
[American Conference of Governmental Industrial Hygienists. Documentation of the Threshold Limit Values and Biological Exposure Indices. 5th ed. Cincinnati, OH:American Conference of Governmental Industrial Hygienists, 1986. 181]**PEER REVIEWED**

... IF INHALED AT 5% BY VOL CONCN INDUCES DIZZINESS IN MAN. IF INHALED AT 15% CONCN, LOSS OF CONSCIOUSNESS RESULTS.
[International Labour Office. Encyclopaedia of Occupational Health and Safety. 4th edition, Volumes 1-4 1998. Geneva, Switzerland: International Labour Office, 1998.,p. 104.184]**PEER REVIEWED**

STUDIES ON ... VOLUNTEERS SHOWED THAT INHALATION OF 10,000 PPM OF FC12 FOR 2.5 HR CAUSES 7% REDUCTION IN STANDARDIZED PSYCHOMOTOR SCORES. AT CONCN OF 1000 PPM FOR 8 HR/DAY, 5 DAYS/WK FOR TOTAL OF 17 REPETITIVE EXPOSURES, THERE WERE NO UNTOWARD SUBJECTIVE RESPONSES & NO ABNORMAL PHYSIOLOGICAL RESPONSES OF LUNGS OR HEART. CONCN AS HIGH AS 27,000 PPM OF FC12 FOR 15 TO 60 SEC CAUSED INCR IN AIRWAY RESISTANCE & ELECTROCARDIOGRAPHIC CHANGES.
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 3091]**PEER REVIEWED**

In brief exptl exposures of humans to F-12 at 198x10+3 mg/cu m vapor concn in air, tingling sensation, humming in the ears, apprehension, EEG and speech changes, and deficits in psychological performance were reported. In other test exposures ... 49x10+3 to 543x10+3 mg/cu m caused cardiac arrhythmia, decreased consciousness, and amnesia or deficits in performance on psychomotor test scores.
[USEPA; Ambient Water Quality Criteria Doc: Halomethanes p.C-47-8 (1980) EPA 440/5-80-051]**PEER REVIEWED**

... Women using fluorocarbon-propellant /incl F-12/ aerosol products and receiving nine or ten times the exposure from normal use /showed/ ... no measureable blood levels of the fluorocarbons or abnormalities in overall health, respiratory, or hematologic parameters.
[USEPA; Ambient Water Quality Criteria Doc: Halomethanes p.C-48 (1980) EPA 440/5-80-051]**PEER REVIEWED**

Refrigeration repair workers may be intermittently exposed to fluorocarbons and their thermal decomposition products. A case of peripheral neuropathy (distal axonopathy) in a commercial refrigeration repairman prompted an epidemiological investigation of the health of refrigeration repair workers. No additional cases of peripheral neuropathy were identified among the 27 refrigeration repair workers studied. A reference group of 14 non-refrigeration repair workers was also studied. No differences were noted between groups for the ulnar (motor and sensory), median (motor and sensory), peroneal, sural, or tibial nerve conduction velocities. Refrigeration repair workers reported palpitations and lightheadedness significantly more often than workers in the reference group. No clinical neurological or electroneurophysiological abnormalities were detected in eight refrigeration repair workers followed up for three years during continuous employment.
[Campbell DD; Br J Ind Med 43 (2): 107-11 (1986)]**PEER REVIEWED**

/UV-B Radiation is likely to incr by ozone depletion caused by atmospheric concentrations of chlorofluorocarbons/. Indications are increasing that UV-B radiation ... plays a role in the induction and growth of cutaneous melanomas, a ... dangerous type of skin cancer. ... There are indications that ... suppression of the immune response by UV-B radiation may occur in humans. The antigen presenting Langerhans cells in the skin are damaged and allergic responses are depressed. ... There are indications that UV-B radiation increases cataract formation, an important cause of blindness especially in areas with limited medical facilities. /Chlorofluorocarbons/
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.101 (1990)]**PEER REVIEWED**

Many gases emitted as a result of industrial and agricultural activities can accumulate in the earth's atmosphere and ultimately contribute to alterations in the vertical distribution and concentrations of stratospheric ozone. Among the most important are those trace gases that have long residence times in the atmosphere. This allows accumulation in the troposphere and a gradual upward migration of the gases into the stratosphere where they contribute to depletion of stratospheric ozone layer. The atmospheric and chemical processes involved are extremely complex. Trace gases of particular concern include certain long lived chlorofluorocarbons, such as CFC-11, CFC-12, and CFC-113. Since the transport of these gases to the stratosphere is slow, their residence times there are long, and the removal processes are slow, any effect on stratospheric ozone already seen is probably the result of anthropogenic emissions of these gases several decades ago. Those gases already in the atmosphere will continue to exert stratospheric ozone depletion effects well into the next century. /Chlorofluorocarbons/
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.47 (1990)]**PEER REVIEWED**

Increased UV-B radiation would be expected to increase photochemical smog, and this would aggravate the related health problems in urban and industrialized areas. /UV-B Radiation/
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.21 (1990)]**PEER REVIEWED**

Deaths resulting from cardiovascular collapse after arrhythmias have been reported after inhalation of Freons 11 and 12.
[Ellenhorn, M.J. and D.G. Barceloux. Medical Toxicology - Diagnosis and Treatment of Human Poisoning. New York, NY: Elsevier Science Publishing Co., Inc. 1988. 528]**PEER REVIEWED**

This study attempted to determine whether exposure to the fluorocarbons chlorodifluoromethane or dichlorodifluoromethane during refrigerator repair caused cardiac arrhythmias. Six repair workers and six plumbers (comparisons) served as study subjects. They kept diaries about their daily activities including exposure to fluorocarbons, physical activity, traffic, meals, smoking, and drinking. Ambulatory electrocardiograms were taken for 24 hours on a day of work exposure and on a comparison day. No cardiac arrhythmias were found to be clearly connected with exposure to fluorocarbons. One subject had several ventricle ectopic beats and a connection with exposure cannot be totally excluded in this worker. Ventricle ectopic beats were somewhat more common in the refrigerator repair workers than in the plumbers. The number of ventricle ectopic beats was low, however, when compared with what is known of ventricle ectopic beats in the normal heart. The average concentrations of fluorocarbons during work were 170 to 815 cu cm/cu m and the peak concentrations were 1300 to 10,000 cu cm/ cu m. In two instances the concentrations remained over 1000 cu cm/cu m for 30 minutes.
[Anttipoika M et al; British Journal of Industrial Medicine 47 (2): 138-40 (1990)]**PEER REVIEWED**

A 68 year old man suffered minor burns of both arms and the chest but not the mouth when he punctured the freezing coil of a refrigerator with an ice pick, thus releasing dichlorodifluoromethane. His clinical course was characterized by severe progressive shock, spiking fever, and death in 96 hr. Autopsy revealed bronchopneumonia and histological changes in the small bronchi thought to represent the effects of degradation products of the compound and of preexisting degenerative disease.
[Hayes, Wayland J., Jr. Pesticides Studied in Man. Baltimore/London: Williams and Wilkins, 1982. 119]**PEER REVIEWED**

Inhalation of Freon compounds at moderate concentrations initially produces CNS anesthetic effects of intoxication and loss of psychomotor coordination. In humans, this effect occurred at levels of 2500 ppm for Freon 113 and 10,000 ppm for Freon 12. Higher concentrations produce marked in coordination, slurring of speech, apprehension, and finally descreasing levels of consciousness. Attendent hypoxia at high concentrations may also produce tremors, convulsions, and cerebral edema. Cardiac sensitization occurs at higher concentrations than initial CNS intoxication.
[Haddad, L.M., Clinical Management of Poisoning and Drug Overdose. 2nd ed. Philadelphia, PA: W.B. Saunders Co., 1990. 1281]**PEER REVIEWED**

The effects of CFC-11 and CFC-12 on human volunteers under controlled conditions /were characterized/. A number of biological end-points, including clinical hematology and chemistry, ECG, EEG, pulmonary function, neurological parameters, and cognitive tests were monitored. Single exposures to CFC-11 or CFC-12 at concentrations of 0.025% (CFC-11, 1.4 g/cu m, CFC-1, 1.2 g/cu m), 0.05% (CFC-11, 2.8 g/cu m, CFC-123, 2.5 g/cu m), and 0.1% (CFC-11, 5.6 g/cu m, CFC-12, 5 g/cu m, for 1 min to 8 hr, induced no observable effects. There was a statistically-significant decrease in cognitive test performance in subjects exposed to CFC-11 at 5.6 g/cu m, 8 hr/day, 5 days/week, for 2-4 weeks, but not in subjects exposed to CFC-12 at 5 g/cu m.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.89 (1990)]**PEER REVIEWED**

In another study, 11 subjects (7 being maintenance technicians of large cooling and refrigerating systems) were exposed for 130 min to CFC-12 (weighted exposure 0.46, 49.9, and 87.7 g/cu m. ... This led to acute reduction of ventilatory lung capacity only at the two highest CFC-12 concentrations, under which conditions a significant decrease in the heart frequency was also observed.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.90 (1990)]**PEER REVIEWED**

Ten subjects /were exposed/ to CFC-11, CFC-12, CFC-114, two mixtures of CFC-11 and CFC-12, and a mixture of CFC-12 and CFC-114 (breathing concentrations between 16 and 150 g/cu m) for 15, 45, or 60 seconds, and found significant acute reduction of ventilatory lung capacity (FEV50, FEF25) on exposure to each chlorofluorocarbon, as well as bradycardia and increased variability in heart rate in seven subjects, negative T-waves in two subjects (one was exposed to CFC-11 and CFC-12), and atrioventricular block in 1 subject (CFC-114). Mixtures exerted stronger respiratory effects than individual chlorofluorocarbon at the same level of exposure.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.90 (1990)]**PEER REVIEWED**

Allergic contact eczema /was reported/ in patch tests performed on three patients that had a prior history of skin reactions to deodorant sprays. All three patients showed strong positive reactions to 11 deodorant sprays and mild to strong reactions to CFC-11. One patient showed a mild reaction to CFC-12. Fifteen controls (without prior history of allergy to deodorants) showed no response to either CFC-11 or CFC-12. These results suggest that individuals may become sensitized to certain chlorofluorocarbons applied repeatedly to the skin surface.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.91 (1990)]**PEER REVIEWED**

Effects of chlorofluorocarbons on bronchiolar tone in asthmatic children /were studied/. Forced expiratory volume, a measure of bronchial tone, was measured in 18 children with a history of asthma, before and after inhaling aerosols of the B2-receptor agonist, fenoterol, or a mixture of CFC-11, CFC-12, and CFC-114, and in the absence of treatment. The levels of exposure were not reported. Exposure to the chlorofluorocarbon mixture significantly reduced forced expiratory volume for 2 hr, relative to "no treatment", and for 8 hr relative to exposure to fenoterol (containing CFC-11 and CFC-12). The results suggest that chlorofluorcarbons can decrease bronchial tone in asthmatic patients, but that this effect is transient and of a sufficiently small magnitude to be superseded by the dilating effects of fenoterol when both fenoterol and chlorofluorcarbon propellants are inhaled together.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.91 (1990)]**PEER REVIEWED**

Death rates among 539 workers exposed occupational in constructing and repairing refrigeration equipment. The chlorofluorocarbons used were CFC-12, HCFC-22, and CFC-502 (a mixture of CFC-115 and HCFC-22). No increase in total deaths (18 cases) was seen among those employed more than 6 months, compared to the expected number (26 cases), nor was there any statistically significant increase in total tumor deaths or deaths caused by lung cancer or cardiovascular diseases. When the study was restricted to those exposed for more than 3 or 10 years, still no significant increases were see. No data on exposure levels were given.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.93 (1990)]**PEER REVIEWED**

Eighty nine workers were examined during their work with refrigerant equipment. The refrigerants used were mainly CFC-12 (in 56% of the cases) and HCFC-22 (32%), the rest being CFC-11, CFC-500 (a mixture of CFC-12 and HCFC 152a), CFC-502 ( a mixture of CFC-115 and HCFC 22). The mean exposure time was 10 min. Chlorofluorocarbon concentrations in the breathing zone were measured for each person individually. The levels exceeded 750 ppm at least once (as one minute mean values) for 60 of the 89 individuals. Cardiac arrhythmias were registered before, during, and after the exposure by means of a portable ECG instrument connected to a tape recorder. No statistically significant difference was found between exposed and nonexposed period, nor was there any dose-related trend for different individuals when grouped into different exposure groups. In this study, possible effects on the central nervous system were also studied by means of simple reaction time measurements before and after the exposure. No impairment was seen.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.93 (1990)]**PEER REVIEWED**

Occasionally liquid or gaseous F12 sprays unexpectedly from a pressurized container or a refrigeratory into a person's eyes, but no significant injuries from this source are reported. Because of the speed of reflex closure of the eyes, it seems extremely unlikely that any serious injury would result from an accidental spray of F-12 in the eyes of conscious or unanesthetized human beings.
[Grant, W.M. Toxicology of the Eye. 3rd ed. Springfield, IL: Charles C. Thomas Publisher, 1986. 322]**PEER REVIEWED**

Five percent is said to induce dizziness.
[Zenz, C. Occupational Medicine-Principles and Practical Applications. 2nd ed. St. Louis, MO: Mosby-Yearbook, Inc, 1988. 542]**PEER REVIEWED**

Ordinary occupational and domestic exposure to the gas causes neither ocular nor respiratory irritation.
[Grant, W.M. Toxicology of the Eye. 3rd ed. Springfield, IL: Charles C. Thomas Publisher, 1986. 322]**PEER REVIEWED**

Propellant /fluorocarbon/ gases were generated from commercial aerosol units and applied to the from distance of 50 cm for periods of 15 to 60 sec. At a measured concn of 95,000 mg/cu m (1700 ppm), there was a biphasic change in ventilation capacity, the first reduction occurring within a few minutes after exposure, and second delayed until 13 to 30 min after exposure, and second delayed until 13 to 30 min after exposure. Most subjects developed bradycardia, and inversion of the T-wave. /Propellant gases/
[Clayton, G.D., F.E. Clayton (eds.) Patty's Industrial Hygiene and Toxicology. Volumes 2A, 2B, 2C, 2D, 2E, 2F: Toxicology. 4th ed. New York, NY: John Wiley & Sons Inc., 1993-1994. 1201]**PEER REVIEWED**

EXCESSIVE SKIN CONTACT WITH LIQ FLUOROCARBONS SHOULD BE MINIMIZED TO PREVENT DEFATTING OF SKIN ... /FLUOROCARBONS/
[International Labour Office. Encyclopedia of Occupational Health and Safety. Vols. I&II. Geneva, Switzerland: International Labour Office, 1983. 897]**PEER REVIEWED**

... The combination of CFC with a sympathomimetic bronchodilator is potentially dangerous for the treatment of bronchial asthma. For the same reason, sympathomimetic drugs are contraindicated in cardiac resuscitation of patients suffering from CFC poisoning. /Fluorocarbon poisoning/
[Clayton, G.D., F.E. Clayton (eds.) Patty's Industrial Hygiene and Toxicology. Volumes 2A, 2B, 2C, 2D, 2E, 2F: Toxicology. 4th ed. New York, NY: John Wiley & Sons Inc., 1993-1994. 1183]**PEER REVIEWED**

Fluorocarbon vapors are 4 to 5 times heavier than air. Thus high concn tend to accumulate in low-lying areas, resulting in hazard of inhalation of concentrated vapors, which may be fatal. /Fluorocarbons/
[Clayton, G.D., F.E. Clayton (eds.) Patty's Industrial Hygiene and Toxicology. Volumes 2A, 2B, 2C, 2D, 2E, 2F: Toxicology. 4th ed. New York, NY: John Wiley & Sons Inc., 1993-1994. 1195]**PEER REVIEWED**

Under certain condition, fluorocarbon vapors may decompose on contact with flames or hot surfaces, creating potential hazard of inhalation of toxic decomposition products. /Fluorocarbons/
[Clayton, G.D., F.E. Clayton (eds.) Patty's Industrial Hygiene and Toxicology. Volumes 2A, 2B, 2C, 2D, 2E, 2F: Toxicology. 4th ed. New York, NY: John Wiley & Sons Inc., 1993-1994. 1195]**PEER REVIEWED**

EARLY ... HUMAN EXPERIENCE INDICATED THAT HIGH VAPOR CONCN (EG, 20%) MAY CAUSE CONFUSION, PULMONARY IRRITATION, TREMORS & RARELY COMA, BUT THAT THESE EFFECTS WERE GENERALLY TRANSIENT & WITHOUT LATE SEQUELAE. ... CAUSE OF DEATH /FROM ABUSE OF FLUOROCARBONS/ IS IN CONSIDERABLE DOUBT. FREEZING OF AIRWAY SOFT TISSUES CAN PROBABLY BE ELIMINATED AS A CAUSE OF DEATH EXCEPT IN CASES WHERE THE PRODUCT WAS SPRAYED DIRECTLY INTO THE MOUTH FROM ITS CONTAINER OR FROM A BALLOON CONTAINING SOME LIQUID. LARYNGEAL SPASM OR EDEMA, OXYGEN DISPLACEMENT, OR SENSITIZATION OF MYOCARDIUM TO ENDOGENOUS CATECHOLAMINES WITH SUBSEQUENT VENTRICULAR FIBRILLATION APPEAR TO BE REASONABLE POSSIBILITIES. /FLUOROCARBON REFRIGERANTS & PROPELLANTS/
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.,p. II-159]**PEER REVIEWED**

Aerosol sprays containing fluorocarbon propellants are another source of solvent intoxication. Prolonged exposure or daily use may result in damage to several organ systems. Clinical problems include cardiac arrhythmias, bone marrow depression, cerebral degeneration, and damage to liver, kidney, & peripheral nerves. Death occasionally has been attributed to inhalant abuse, probably via the mechanism of cardiac arrhythmias, especially accompanying exercise or upper airway obstruction. /Fluorocarbon propellants/
[Hardman, J.G., L.E. Limbird, P.B. Molinoff, R.W. Ruddon, A.G. Goodman (eds.). Goodman and Gilman's The Pharmacological Basis of Therapeutics. 9th ed. New York, NY: McGraw-Hill, 1996. 575]**PEER REVIEWED**

A SPECIAL CLASS OF CHEMICALS SUBJECT TO ABUSE BY INHALATION ARE THE FLUOROHYDROCARBONS ... THE "SNIFFING" OF SUCH AEROSOL SPRAYS IS HAZARDOUS PRACTICE. ... 110 "SUDDEN SNIFFING DEATHS" /HAVE BEEN IDENTIFIED/ ... IN EACH CASE THE VICTIM SPRAYED THE AEROSOL INTO A PLASTIC BAG, INHALED THE CONTENTS, BECAME EXCITED, RAN 90 M OR SO, COLLAPSED, & DIED. NECROPSY FINDINGS WERE LARGELY NEGATIVE ... ALTHOUGH AMOUNT OF PROPELLANT ABSORBED INTO BLOOD FROM USE OF HAIRSPRAY, COSMETIC, HOUSEHOLD, & MEDICATED AEROSOLS MUST VARY WITH CIRCUMSTANCES, PHYSICIAN IS ADVISED TO COUNSEL ... PATIENT ON POTENTIAL DANGERS, PARTICULARLY FROM THEIR USE IN POORLY VENTILATED CONFINED AREAS. IT IS POSSIBLE THAT PATIENTS WITH CARDIAC OR RESPIRATORY DISORDERS MAY PROVE ESPECIALLY SUSCEPTIBLE. /FLUOROHYDROCARBONS/
[Goodman, L.S., and A. Gilman. (eds.) The Pharmacological Basis of Therapeutics. 5th ed. New York: Macmillan Publishing Co., Inc., 1975. 910]**PEER REVIEWED**

Fluorocarbons were initially believed to be compounds low in toxicity. In the late 1960s there were early reports of deaths caused by intentional inhalation abuse of various aerosols. Victims frequently discharged the aerosol contents into a plastic bag and then inhaled the gaseous contents. Suffocation was initially considered to be the cause of death. In 1970, 110 cases of "sudden sniffing death" /were reviewed/ without finding evidence of suffocation. The majority of those deaths (59) involved fluorocarbon propellants. He noted that in several cases sudden death followed a burst of emotional stress or exercise. No significant findings were noted at autopsy. /Fluorocarbons/
[Haddad, L.M., Clinical Management of Poisoning and Drug Overdose. 2nd ed. Philadelphia, PA: W.B. Saunders Co., 1990. 1281]**PEER REVIEWED**

Fluorocarbon propellants are anesthetic and cardiotoxic. ... Aerosol propellants produce hallucinogenic effects, and, rarely, contact dermatitis. /Fluorocarbon propellants/
[Ellenhorn, M.J. and D.G. Barceloux. Medical Toxicology - Diagnosis and Treatment of Human Poisoning. New York, NY: Elsevier Science Publishing Co., Inc. 1988. 528]**PEER REVIEWED**

Fluorocarbon propellants, benzene, 1,1,1-trichloroethane, gasoline, toluene, and hydrocarbons have been implicated in 110 sudden deaths after inhalant abuse in which no obvious cardiac or pulmonary pathology existed. Heavy exercise or stress was associated with 18 of those deaths, /it was/ proposed that these inhalants act to sensitize the myocardium to endogenous catecholamines. Hypoxia, hypercarbia, and acidosis may exacerbate these effects. /Fluorocarbon propellants/
[Ellenhorn, M.J. and D.G. Barceloux. Medical Toxicology - Diagnosis and Treatment of Human Poisoning. New York, NY: Elsevier Science Publishing Co., Inc. 1988. 841]**PEER REVIEWED**

Chlorinated hydrocarbons may cause systemic toxicity through percutaneous absorption. Systemic toxicity includes convulsion, delirium, and central nervous system depression /From table/. /Chlorinated hydrocarbons/
[Zenz, C. Occupational Medicine-Principles and Practical Applications. 2nd ed. St. Louis, MO: Mosby-Yearbook, Inc, 1988. 160]**PEER REVIEWED**

There are isolated reports of poisoning from exposure to refrigerants and solvents, and some studies showing a higher incidence of coronary heart disease among hospital personnel are required to establish causal relationship between fluorine containing organic compounds, and cardiovascular and bronchopulmonary diseases among exposed workers. The high incidence of cancer among hospital personnel repeatedly exposed to fluorine-containing general anesthetics raises a fundamental need to examine other chlorofluorocarbon-exposed workers for similar effects. /Fluorocarbons/
[Clayton, G.D., F.E. Clayton (eds.) Patty's Industrial Hygiene and Toxicology. Volumes 2A, 2B, 2C, 2D, 2E, 2F: Toxicology. 4th ed. New York, NY: John Wiley & Sons Inc., 1993-1994. 1209]**PEER REVIEWED**

Clinical pathologists exposed to fluorocarbons in the preparation of frozen tissue sections have been seen to develop coronary heart disease. /Fluorocarbons/
[Clayton, G.D., F.E. Clayton (eds.) Patty's Industrial Hygiene and Toxicology. Volumes 2A, 2B, 2C, 2D, 2E, 2F: Toxicology. 4th ed. New York, NY: John Wiley & Sons Inc., 1993-1994. 1209]**PEER REVIEWED**

There is undisputed evidence that the atmospheric concentrations of chlorofluorocarbons deplete ozone in the stratosphere. A reduction in ozone concentration will result in increased transmission of solar ultraviolet radiation through the stratosphere. Many significant adverse effects of such an increase in exposure to this radiation have been identified. ... One of the most well-defined human health effects resulting from stratospheric ozone depletion is an increase in the frequency of skin cancer expected as a result of even small increases in UV-B radiation (280-320 nm) reaching the earhs's surface. /Chlorofluorocarbons/
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.95 (1990)]**PEER REVIEWED**

Freons are toxic to humans by several mechanisms. Inhaled fluorocarbons sensitized the myocardium to catecholamines, frequently resulting in lethal ventricular arrhythmias. Because they are gases heavier than air, fluorocarbons can displace atmospheric oxygen, thus resulting in asphyxiation. These compounds also have a central nervous system (CNS) anesthetic effect analogous to a structurally similar general anesthetic, halothane. Pressurized refrigerant or liquid fluorocarbons with a low boiling point have a cryogenic effect on exposed tissues, causing frostbite, laryngeal or pulmonary edema, and gastrointestinal perforation. Certain fluorocarbons degrade at high temperatures into toxic products of chlorine, hydrofluoric acid, or phosgene gases. /Freons/
[Haddad, L.M., Clinical Management of Poisoning and Drug Overdose. 2nd ed. Philadelphia, PA: W.B. Saunders Co., 1990. 1281]**PEER REVIEWED**

... HIGH VAPOR CONCN (EG, 20%) MAY CAUSE CONFUSION, PULMONARY IRRITATION, TREMORS & RARELY COMA ... BUT ... THESE EFFECTS WERE GENERALLY TRANSIENT & WITHOUT LATE SEQUELAE. /FLUOROCARBON REFRIGERANTS & PROPELLANTS/
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.,p. II-159]**PEER REVIEWED**

Non-occupational exposure and accidental or abusive inhalation of aerosols /due to Fluorocarbon propellants/ have also been documented, the main symptoms being CNS depression and cardiovascular reactions. Cardiac arrhythmia, possibly aggravated by elevated levels of catecholamines due to stress or by moderate hypercapnia, is suggested as the cause of these adverse response, which may lead to death. /Aerosols/
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.20 (1990)]**PEER REVIEWED**

Manufacturing processes use hydrofluoric acid from fluorospar in the production of most fluorine containing organic compounds. Some processes use carbon tetrachloride from carbon disulfide or as a co product of perchloroethylene and chlorination of propylene, or chloroform from chlorination of methanol. The major hazards relate primarily to the inadvertent release of hydrofluoric acid or carbon tetrachloride, rather than to the manufactured final product. /Fluorocarbons/
[Clayton, G.D., F.E. Clayton (eds.) Patty's Industrial Hygiene and Toxicology. Volumes 2A, 2B, 2C, 2D, 2E, 2F: Toxicology. 4th ed. New York, NY: John Wiley & Sons Inc., 1993-1994. 1194]**PEER REVIEWED**

... CAUSE OF DEATH /FROM ABUSE OF FLUOROCARBONS/ IS IN ... DOUBT. FREEZING OF AIRWAY SOFT TISSUES CAN PROBABLY BE ELIMINATED ... EXCEPT IN CASES WHERE PRODUCT WAS SPRAYED DIRECTLY INTO MOUTH FROM CONTAINER OR BALLOON CONTAINING SOME LIQ. /FLUOROCARBON REFRIGERANTS & PROPELLANTS/
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.,p. II-159]**PEER REVIEWED**

... CAUSE OF DEATH /FROM ABUSE OF FLUOROCARBONS/ ... IN ... DOUBT. ... LARYNGEAL SPASM OR EDEMA, OXYGEN DISPLACEMENT, OF SENSITIZATION OF MYOCARDIUM TO ENDOGENOUS CATECHOLAMINES WITH ... VENTRICULAR FIBRILLATION APPEAR TO BE ... POSSIBILITIES. /FLUOROCARBON REFRIGERANTS & PROPELLANTS/
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.,p. II-159]**PEER REVIEWED**

The toxicity of Chlorofluorocarbons (CFCs) had been considered to be low; it is absorbed via the lungs and undergoes little subsequent biotransformation. In the United States when sudden unexplained deaths of aerosol "sniffers" were reported they were considered to be possibly due to cardiac arrhythmias induced by the CFC propellants. /CFCs/
[Rom, W.N. (ed.). Environmental and Occupational Medicine. 2nd ed. Boston, MA: Little, Brown and Company, 1992. 1299]**PEER REVIEWED**

Speizer and coworkers reported that pathology personnel exposed to FC-22 and FC-12 /dichlorodifluoromethane/ while preparing frozen sections had a greater prevalence of palpitations than an unexposed control group; ... Continuous electrocardiographic (EKG) monitoring of several exposed subjects in the course of their work showed multiple arrhythmias.
[Rom, W.N. (ed.). Environmental and Occupational Medicine. 2nd ed. Boston, MA: Little, Brown and Company, 1992. 1300]**PEER REVIEWED**

Studies on two human volunteers who inhaled CFC-12 at 10,000 ppm for 2.5 hours showed a 7% reduction in a standardized psychomotor test score but no other adverse effects.
[American Conference of Governmental Industrial Hygienists, Inc. Documentation of the Threshold Limit Values and Biological Exposure Indices. 6th ed. Volumes I,II, III. Cincinnati, OH: ACGIH, 1991. 421]**PEER REVIEWED**

Blood levels of CFC-12 were below detection limits in normal subjects using household aerosols; in asthmatic subjects using an aerosol inhaler, blood levels were much lower than in dogs exposed at the threshold for cardiac sensitization. Radiolabeled tests showed essentially all the dose of CFC-12 (95%-103%) exhaled within the first hour after a 12 or 17 minute inhalation at 1000 ppm.; total metabolites were <0.2% of the administered dose. At 30 minutes, retention of the labeled dose inhaled in a single breath was 10% versus 23%, 20%, & 12% for comparable doses of trichlorofluoromethane (FC-11), 1,1,2-trichloro-1,2,2-trifluoroethane (FC-113), & 1,2-dichloro-1,1,2,2-tetrafluoroethane (FC-114), respectively. For an 8 hr inhalation at 1000 ppm, a pharmacokinetic model based on analyses in dogs & humans gave an estimate of 55% absorption of the inhaled CFC-12. This dose would result in roughly 1/20th the blood level required to sensitize dogs also receiving the stress of IV epinephrine.
[American Conference of Governmental Industrial Hygienists, Inc. Documentation of the Threshold Limit Values and Biological Exposure Indices. 6th ed. Volumes I,II, III. Cincinnati, OH: ACGIH, 1991. 421]**PEER REVIEWED**

Following exposure to CFC-12 at a concentration of 1% (50 g/cu m) for 150 min, a 7% decrease in psychomotor test scores was noted, but no effects were observed at 0.1% (5 g/cu m).
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.19 (1990)]**PEER REVIEWED**


Medical Surveillance:

Employees should be screened for history of certain medical conditions ... which might place the employee at increased risk from dichlorodifluoromethane exposure. Cardiovascular disease: In persons with impaired cardiovascular function, especially those with a history of cardiac arrhythmias, the inhalation of dichlorodifluoromethane might cause exacerbation of disorders of the conduction mechanism due to its sensitizing effects on the myocardium. ... Any employee developing the above-listed conditions should be referred for further medical examination.
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) PublicationNo. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981. 1]**PEER REVIEWED**


Populations at Special Risk:

Employees /with cardiovascular disease are/ at increased risk.
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) PublicationNo. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981. 1]**PEER REVIEWED**

IT IS POSSIBLE THAT PT WITH CARDIAC OR RESP DISORDERS MAY PROVE ESP SUSCEPTIBLE. /FLUOROCARBONS/
[Goodman, L.S., and A. Gilman. (eds.) The Pharmacological Basis of Therapeutics. 5th ed. New York: Macmillan Publishing Co., Inc., 1975. 910]**PEER REVIEWED**


Probable Routes of Human Exposure:

NIOSH (NOES Survey 1981-1983) has statistically estimated that 435,098 workers (125,602 of these are female) are potentially exposed to dichlorodifluoromethane in the US(1). Occupational exposure may be through inhalation and dermal contact with this compound at workplaces where dichlorodifluoromethane is still used, such as air conditioning repair shops(SRC). This survey was conducted prior to the Montreal Protocol which scheduled the production phase-out of this compound and other chlorofluorocarbons, and is not an accurate measure of the current occupational exposure(SRC). Due to its long atmospheric residence time, the general population may be exposed to dichlorodifluoromethane via inhalation of ambient air(SRC).
[(1) NIOSH; National Occupational Exposure Survey (NOES) (1983)]**PEER REVIEWED**


Body Burden:

In a pilot study of pollutants in the milk of women living in 4 urban-industrial areas in the US, dichlorodifluoromethane was identified, not quantified, in 2 of 8 samples(1).
[(1) Pellizzari ED et al; Bull Environ Contam Toxicol 28: 322-8 (1982)]**PEER REVIEWED**


Emergency Medical Treatment:

Emergency Medical Treatment:

EMT Copyright Disclaimer:
Portions of the POISINDEX(R) database are provided here for general reference. THE COMPLETE POISINDEX(R) DATABASE, AVAILABLE FROM MICROMEDEX, SHOULD BE CONSULTED FOR ASSISTANCE IN THE DIAGNOSIS OR TREATMENT OF SPECIFIC CASES. Copyright 1974-1998 Micromedex, Inc. Denver, Colorado. All Rights Reserved. Any duplication, replication or redistribution of all or part of the POISINDEX(R) database is a violation of Micromedex' copyrights and is strictly prohibited.

The following Overview, *** FLUORINATED HYDROCARBONS ***, is relevant for this HSDB record chemical.

Life Support:
  o   This overview assumes that basic life support measures
      have been instituted.                           
Clinical Effects:
  SUMMARY OF EXPOSURE
   0.2.1.1 ACUTE EXPOSURE
     o   LOW CONCENTRATION - Inhalations such as those caused by
         leaking air conditioners or refrigerators usually
         result in transient eye, nose, and throat irritation.
         Palpitations,  light headedness, and headaches are also
         seen.
     o   HIGH CONCENTRATION - Inhalation associated with
         deliberate abuse, or spills or industrial use occurring
         in poorly ventilated areas has been associated with
         ventricular arrhythmias, pulmonary edema and sudden
         death.
  HEENT
   0.2.4.1 ACUTE EXPOSURE
     o   EYES - Eye irritation occurs with ambient exposure.
         Frostbite of the lids may be severe.  Ocular
         instillation results in corneal burns in rabbits.
     o   NOSE - Nasal irritation occurs with ambient exposure.
     o   THROAT - Irritation occurs.  Frostbite of the lips,
         tongue, buccal  mucosa and hard palate developed in a
         man after deliberate inhalation.
  CARDIOVASCULAR
   0.2.5.1 ACUTE EXPOSURE
     o   Inhalation of high concentrations is associated with
         the development of refractory ventricular arrhythmias
         and sudden death, believed to be secondary, primarily,
         to myocardial sensitization to endogenous
         catecholamines.  Some individuals may be susceptible to
         arrhythmogenic effects at lower concentrations.
  RESPIRATORY
   0.2.6.1 ACUTE EXPOSURE
     o   Pulmonary irritation, bronchial constriction, cough,
         dyspnea, and chest tightness may develop after
         inhalation.  Chronic pulmonary hyperreactivity may
         occur.  Adult respiratory distress syndrome has been
         reported following acute inhalational exposures.
         Pulmonary edema is an  autopsy finding in fatal cases.
  NEUROLOGIC
   0.2.7.1 ACUTE EXPOSURE
     o   Headache, dizziness, and disorientation are common.
         Cerebral edema may be found on autopsy.  A syndrome of
         impaired psychomotor speed, impaired memory and
         learning, and emotional lability has been described in
         workers with chronic occupational exposure to
         fluorinated hydrocarbons.
  GASTROINTESTINAL
   0.2.8.1 ACUTE EXPOSURE
     o   Nausea may develop.  Ingestion of a small amount of
         trichlorofluoromethane resulted in necrosis and
         perforation of the stomach in one patient.
  HEPATIC
   0.2.9.1 ACUTE EXPOSURE
     o   Jaundice and mild elevations in transaminases may
         develop after  inhalational exposure or ingestion.
         Hepatocellular coagulative necrosis has been observed
         on liver biopsy.
  DERMATOLOGIC
   0.2.14.1 ACUTE EXPOSURE
     o   Dermal contact may result in defatting, irritation or
         contact dermatitis.  Severe frostbite has been reported
         as an effect of freon exposure.  Injection causes
         transient pain, erythema and edema.
  MUSCULOSKELETAL
   0.2.15.1 ACUTE EXPOSURE
     o   Rhabdomyolysis has been reported in a worker
         susceptible to malignant hyperthermia after exposure to
         fluorinated hydrocarbons and also following intentional
         freon inhalation.  Compartment syndrome is a rare
         complication of severe exposure.
  REPRODUCTIVE HAZARDS
    o   Dichlorodifluoromethane was not teratogenic in rats and
        rabbits.
    o   The reproductive effects of 1,1,1,2-tetrafluoroethane
        were studied in rats.  No adverse effects on
        reproductive performance was noted or on the
        development, maturation or reproductive performance of
        up to two successive generations.
  GENOTOXICITY
    o   The hydrochlorofluorocarbons, HCFC-225ca and HCFC-225cb,
        were not mutagenic in the Ames reverse mutation assay,
        or clastogenic in the chromosomal  aberration assay with
        Chinese hamster lung cells.  Neither induced unscheduled
        DNA synthesis in liver cells.  Both of these agents were
        clastogenic in the chromosomal aberration assay with
        human lymphocytes.                        
Laboratory:
  o   Fluorinated hydrocarbons plasma levels are not clinically
      useful.
  o   No specific lab work (CBC, electrolyte, urinalysis) is
      needed unless otherwise indicated.
  o   Obtain baseline pulse oximetry or arterial blood gas
      analysis.
Treatment Overview:
  SUMMARY EXPOSURE
    o   Monitor EKG and vital signs carefully.  Cardiopulmonary
        resuscitation may be necessary.
  ORAL EXPOSURE
    o   These substances may cause frostbite to the upper airway
        and gastrointestinal  tract after ingestion.  Administer
        oxygen and manage airway as clinically  indicated.
        Emesis, activated charcoal, and gastric lavage are not
        recommended.
  INHALATION EXPOSURE
    o   MONITOR ECG and VITAL SIGNS carefully.  Cardiopulmonary
        resuscitation may be necessary.  AVOID CATECHOLAMINES.
    o   PROVIDE A QUIET CALM ATMOSPHERE to prevent adrenaline
        surge if the patient is seen before the onset of cardiac
        arrhythmias.  Minimize physical exertion.
    o   MONITOR pulse oximetry or arterial blood gases.
    o   Provide symptomatic and supportive care.
    o   These substances may cause frostbite of the upper airway
        with the potential for  severe edema.  Administer oxygen
        and manage airway early in patients with evidence  of
        upper airway injury.
    o   PULMONARY EDEMA (NONCARDIOGENIC):  Maintain ventilation
        and oxygenation and evaluate with frequent arterial
        blood gas or pulse oximetry monitoring.  Early use of
        PEEP and mechanical ventilation may be needed.
  EYE EXPOSURE
    o   DECONTAMINATION:  Irrigate exposed eyes with copious
        amounts of tepid water for at least 15 minutes.  If
        irritation, pain, swelling, lacrimation, or photophobia
        persist, the patient should be seen in a health care
        facility.
    o   Ophthamologic consultation should be obtained in any
        symptomatic patients.
  DERMAL EXPOSURE
    o   DECONTAMINATION:  Remove contaminated clothing and wash
        exposed  area thoroughly with soap and water.  A
        physician may need to  examine the area if irritation or
        pain persists.
    o   If frostbite has occurred, refer to dermal treatment in
        the main body of this document for rewarming.
Range of Toxicity:
  o   Freons are very toxic when inhaled in high concentrations
      and/or for extended periods.  At lower concentrations or
      brief exposure, freons may cause transient eye, nose, and
      throat irritation.  There is significant interpatient
      variation and it is difficult to predict which patient
      will exhibit symptoms following exposure.            

[Rumack BH: POISINDEX(R) Information System. Micromedex, Inc., Englewood, CO, 2001; CCIS Volume 110, edition exp November, 2001. Hall AH & Rumack BH (Eds):TOMES(R) Information System. Micromedex, Inc., Englewood, CO, 2001; CCIS Volume 110, edition exp November, 2001.] **PEER REVIEWED**

Antidote and Emergency Treatment:

Treatment is entirely symptomatic.
[Hayes, Wayland J., Jr. Pesticides Studied in Man. Baltimore/London: Williams and Wilkins, 1982. 119]**PEER REVIEWED**

... Emergency treatment is supportive and includes decontamination, oxygen, and any specific therapy required in a particular case such as antiarrhythmics or anticonvulsants. A few patients may require intermittent positive-pressure ventilation, dialysis, or treatment for hepatic failure. /Solvent abuse/
[Haddad, L.M., Clinical Management of Poisoning and Drug Overdose. 2nd ed. Philadelphia, PA: W.B. Saunders Co., 1990. 1259]**PEER REVIEWED**

... In persons who are intoxicated with fluorocarbons, steps can be taken to lessen the risk of arrhythmias. ... Before evaluation at the hospital, patients should be advised to avoid strenuous exercise. In the hospital, patients can be placed in a quiet, nonthreatening environment and sedated if necessary. If hypoxic, oxygen should be administered and metabolic abnormalities corrected. Sympathomimetic drugs should be avoided. Ventricular arrhythmias are best treated with beta-blocking agents. /Fluorocarbons/
[Haddad, L.M., Clinical Management of Poisoning and Drug Overdose. 2nd ed. Philadelphia, PA: W.B. Saunders Co., 1990. 81]**PEER REVIEWED**

Patients with fluorohydrocarbon poisoning should not be given epinephrine (Adrenalin) or similar drugs because of the tendency of fluorohydrocarbon to induce cardiac arrhythmia, including ventricular fibrillation. /Fluorohydrocarbons/
[Zenz, C. Occupational Medicine-Principles and Practical Applications. 2nd ed. St. Louis, MO: Mosby-Yearbook, Inc, 1988. 543]**PEER REVIEWED**

Victims of Freon inhalation require management for hypoxic, CNS anesthetic, and cardiac symptoms. Patients must be removed from the exposure environment, and high-flow supplemental oxygen should be utilized. The respiratory system should be evaluated for injury, aspiration, or pulmonary edema and treated appropriately. CNS findings should be treated supportively. A calm environment with no physical exertion is imperative to avoid increasing endogenous adrenegic levels. Exogenous adrenergic drugs must not be used to avoid inducing sensitized myocardial dysrhythmias. Atropine is ineffective in treating bradyarrhythmias. For ventricular dysrhythmias, diphenylhydantoin and countershock may be effective. Cryogenic dermal injuries should be treated by water bath rewarming at 40 to 42 deg C until vasodilatory flush has returned. Elevation of the limb and standard frostbite management with late surgical debridement should be utilized. Ocular exposure requires irrigation and slit-lamp evaluation for injury. /Freons/
[Haddad, L.M., Clinical Management of Poisoning and Drug Overdose. 2nd ed. Philadelphia, PA: W.B. Saunders Co., 1990. 1282]**PEER REVIEWED**

... IF INHALATION OCCURS, EPINEPHRINE OR OTHER SYMPATHOMIMETIC AMINES & ADRENERGIC ACTIVATORS SHOULD NOT BE ADMIN SINCE THEY WILL FURTHER SENSITIZE HEART TO DEVELOPMENT OF ARRHYTHMIAS. /FLUOROCARBONS/
[Clayton, G.D., F.E. Clayton (eds.) Patty's Industrial Hygiene and Toxicology. Volumes 2A, 2B, 2C, 2D, 2E, 2F: Toxicology. 4th ed. New York, NY: John Wiley & Sons Inc., 1993-1994. 1201]**PEER REVIEWED**

Basic treatment: Establish a patent airway. Suction if necessary. Watch for signs of respiratory insufficiency and assist ventilations as needed. Administer oxygen by nonrebreather mask at 10 to 15 L/min. Minimize physical activity and provide a quiet atmosphere. Monitor for pulmonary edema and treat if necessary ... . Anticipate seizures and treat if necessary ... . For eye contamination, flush eyes immediately with water. Irrigate each eye continuously with normal saline during transport ... . Do not use emetics. Rinse mouth and administer 5 ml/kg up to 200 ml of water for dilution if the patient can swallow, has a strong gag reflex, and does not drool. Administer activated charcoal ... . Treat frostbite with rapid rewarming techniques ... . /Chlorinated fluorocarbons (CFCs) and related compounds/
[Bronstein, A.C., P.L. Currance; Emergency Care for Hazardous Materials Exposure. 2nd ed. St. Louis, MO. Mosby Lifeline. 1994. 192]**PEER REVIEWED**

Advanced treatment: Consider orotracheal or nasotracheal intubation for airway control in the patient who is unconscious or in respiratory arrest. Positive pressure ventilation techniques with a bag valve mask device may be beneficial. Monitor cardiac rhythm and treat arrhythmias if necessary ... . Start an IV with D5W /SRP: "To keep open", minimal flow rate/. Use lactated Ringer's if signs of hypovolemia are present. Watch for signs of fluid overload. Consider drug therapy for pulmonary edema ... . Treat seizures with diazepam ... . Use proparacaine hydrochloride to assist eye irrigation ... . /Chlorinated fluorocarbons (CFCs) and related compounds/
[Bronstein, A.C., P.L. Currance; Emergency Care for Hazardous Materials Exposure. 2nd ed. St. Louis, MO. Mosby Lifeline. 1994. 192]**PEER REVIEWED**


Animal Toxicity Studies:

Non-Human Toxicity Excerpts:

... ABOVE 3000 PPM ... ADMIN TO RATS 4 HR/DAY FOR 10 DAYS, CAUSED SLIGHT LIVER AND BONE MARROW INJURY.
[Thienes, C., and T.J. Haley. Clinical Toxicology. 5th ed. Philadelphia: Lea and Febiger, 1972. 150]**PEER REVIEWED**

... TWITCHING & TREMORS /OBSERVED IN RATS AT/ CONCN OF 30-40%, LOSS OF REFLEX AT 50% & ABOVE. AT 70 & 80%, CORNEAL REFLEX WAS ABSENT & ANIMALS WERE IN DEEP ANESTHESIA. A FEW OF THE ANIMALS WERE EXPOSED AS LONG AS 4-6 HR AT 80% AND ... SUFFERED NO PERMANENT EFFECTS.
[Patty, F. (ed.). Industrial Hygiene and Toxicology: Volume II: Toxicology. 2nd ed. New York: Interscience Publishers, 1963. 1325]**PEER REVIEWED**

... Dogs, monkeys, rats, rabbits & guinea pigs /had continuous exposure/ to 810 ppm of FC 12, 24 hr daily for 90 days. No deaths were attributed to exposure and pathologic changes ... occurred only in guinea pigs, who showed microscopic liver injury. ... At 200,000 ppm, guinea pigs, dogs and monkeys exposed some 40 hr weekly for 10-12 weeks showed generalized tremors and other signs of mild /CNS depression/, as well as slight blood changes, but no pathologic effects.
[American Conference of Governmental Industrial Hygienists. Documentation of the Threshold Limit Values and Biological Exposure Indices. 5th ed. Cincinnati, OH:American Conference of Governmental Industrial Hygienists, 1986. 181]**PEER REVIEWED**

DOGS, MONKEYS, & GUINEA PIGS EXPOSED TO 20% OF GAS IN AIR FOR SEVERAL HR A DAY FOR SEVERAL DAYS SHOWED TEMPORARY INTOXICATION WITH TREMORS, ATAXIA, AND TENDENCY TO STARE, SALIVATE, & LACRIMATE, BUT NO CUMULATIVE TOXIC EFFECT & NO SPECIFIC OCULAR DISTURBANCE.
[Grant, W.M. Toxicology of the Eye. 3rd ed. Springfield, IL: Charles C. Thomas Publisher, 1986. 322]**PEER REVIEWED**

In gaseous or vapor form at room temperature or body temperature, F 12 has very little toxicity to the eye, inside or outside. A bubble of the gas injected into the anterior chamber of rabbit eyes has not proved damaging to the cornea. Exposure of a rabbit eye to pure F 12 gas at room temperature for one and one-half minutes induced a slight irregularity of the corneal epithelium, but the eye was completely normal the next day.
[Grant, W.M. Toxicology of the Eye. 3rd ed. Springfield, IL: Charles C. Thomas Publisher, 1986. 323]**PEER REVIEWED**

MICE EXPOSED FOR 3 OR 6 WK, 30 MIN/DAY TO 40,000 PPM, SHOWED ... RESP PATHOLOGY. RATS FED 160 TO 379 MG/KG, 5 DAYS/WK FOR 18 WK, SHOWED ... SLIGHT ELEVATION OF PLASMA ALKALINE PHOSPHATASE.
[American Conference of Governmental Industrial Hygienists. Documentation of the Threshold Limit Values and Biological Exposure Indices. 5th ed. Cincinnati, OH:American Conference of Governmental Industrial Hygienists, 1986. 181]**PEER REVIEWED**

... ADMIN BY GAVAGE TO /GROUPS OF 50 MALE & FEMALE ALBINO/ RATS AT 15 OR 150 MG/KG/DAY FOR 2 YR, /CONTROL GROUPS OF 50 RATS OF EACH SEX RECEIVED VEHICLE BY INTUBATION/; & IN FOOD TO BEAGLE DOGS AT 8 OR 80 MG/KG/DAY FOR 2 YR. EXCEPT FOR SLIGHT ADVERSE EFFECT ON GROWTH OF RATS THAT RECEIVED HIGH DOSES, NO CLINICAL, BIOCHEMICAL, URINARY, HEMATOLOGICAL, OR HISTOPATHOLOGICAL CHANGES ... FOUND IN EITHER SPECIES. NO EVIDENCE OF CARCINOGENIC RESPONSE ... OBSERVED.
[National Research Council. Drinking Water and Health. Volume 3. Washington, DC: National Academy Press, 1980. 103]**PEER REVIEWED**

IN CHRONIC EXPOSURE STUDIES, MALE & FEMALE RATS ... WERE GIVEN ... DOSES OF 15 OR 150 MG/KG BY INTUBATION. THE RATS WERE THEN BRED & EVALUATED FOR FERTILITY, CORPORA LUTEA, IMPLANTATION SITES, RESORPTION SITES, & NUMBER OF LIVE FETUSES/LITTER. NO DOMINANT LETHALITY WAS FOUND AT EITHER DOSE LEVEL.
[National Research Council. Drinking Water and Health. Volume 3. Washington, DC: National Academy Press, 1980. 103]**PEER REVIEWED**

FREON 12 WAS NOT MUTAGENIC TO CHINESE HAMSTER OVARY CELLS IN THE PRESENCE OR ABSENCE OF ACTIVATION SYSTEM.
[KRAHN DF; BANBURY REP 2 (MAMM CELL MUTAGEN: MATURATION TEST SYSTEM) 251-61 (1979)]**PEER REVIEWED**

EFFECTS OF FC 12 ON ELECTRICAL ACTIVITY OF CELLS IN ATRIAL & VENTRICULAR MYOCARDIUM OF ANESTHETIZED RATS WERE RECORDED. MAJOR DECREASE IN DIASTOLIC POTENTIAL, AMPLITUDE OF ACTION POTENTIAL, & MODIFICATION IN SHAPE OF THE ACTION POTENTIAL WAS OBSERVED. RHYTHM ABNORMALITIES CONSISTED OF A DECR IN ATRIOVENTRICULAR CONDUCTION & CHANGES IN THE MYOCARDIAL PASSIVE EXCITABILITY. CARDIOTOXICITY OF FC 12 IS ASSUMED TO AFFECT PASSIVE OR ACTIVE TRANSMEMBRANE IONIC MOVEMENTS.
[LESSARD Y ET AL; CR SEANCES SOC BIOL SES FIL 174 (1): 52-7 (1980)]**PEER REVIEWED**

Pathologic liver changes were reported in guinea pigs chronically exposed (continued for 90 days; or eight hr daily, 5 days weekly, for six wk) to F 12 at levels of about 4,000 mg/cu m (0.08 % by vol).
[USEPA; Ambient Water Quality Criteria Doc: Halomethanes p.C-52-5 (1980) EPA 440/5-80-051]**PEER REVIEWED**

In one chronic (90 day) feeding study of F-12 in rats at 35 and 350 mg/kg/day ... somewhat elevated urinary fluoride and plasma alkaline phosphatase levels. No changes in dogs at 10 and 100 mg/kg/day were observed.
[USEPA; Ambient Water Quality Criteria Doc: Halomethanes p.C-56 (1980) EPA 440/5-80-051]**PEER REVIEWED**

In a two-yr study using rats intubated with F 12 in corn oil at 15 and 150 mg/kg/day ... some suppresssion of wt gain at the high dose level, but no effects with respect to clinical signs, liver function, hematology, or histopathology /were observed/.
[USEPA; Ambient Water Quality Criteria Doc: Halomethanes p.C-59 (1980) EPA 440/5-80-051]**PEER REVIEWED**

Significant mutagenic activity of F 12 at 2.47x10+6 mg/cu m (50%) in air in a Neurospora crassa test system.
[USEPA; Ambient Water Quality Criteria Doc: Halomethanes p.C-59 (1980) EPA 440/5-80-051]**PEER REVIEWED**

The relative potency of effect of a wide range of halogenated and unsubstituted hydrocarbons on the CNS and heart of experimental animals were determined. The chemicals used caused stimulation or depression of the rat CNS after 10 min inhalation at 0.24-80% (vol/vol), and cardiac sensitization in dogs after 5 minute inhalation at 0.12-80% (vol/vol). The toxicity could not be correlated with chemical structure, mol wt, the presence or absence of various halogens or the degree of saturation, but it was inversely related to the saturated vapor pressure. When the results were expressed on a thermodynamic scale, the chemicals had similar potencies at relative saturation of 0.004-0.04. The effects of these chemicals on the CNS and heart are probably structurally non-specific, and the chemical may be regarded as ... toxicants whose effects are predictable from their physico-chemical properties.
[Clark DG, Tinston DJ; Human Toxicol 1 (3): 239-47 (1982)]**PEER REVIEWED**

... INSEMINATED WISTAR ALBINO RATS & ALBINO RABBITS ... /WERE/ ADMIN MIXT OF 90% FREON 12 ... & 10% FREON 11 BY INHALATION FOR 2 HR/DAY. RATS WERE EXPOSED ON DAYS 4 TO 16 OF GESTATION & RABBITS ON DAYS 5 TO 20. MIXT WAS ADMIN IN 20% CONCN (200,000 PPM). NO INDICATIONS OF ANY EMBRYOTOXIC, FETOTOXIC OR TERATOGENIC CHANGES WERE FOUND WHEN DAMS WERE SACRIFICED & FETUSES REMOVED ON DAY 20 OF GESTATION (RATS) OR DAY 30 OF GESTATION (RABBITS).
[National Research Council. Drinking Water and Health. Volume 3. Washington, DC: National Academy Press, 1980. 103]**PEER REVIEWED**

CFC-11, CFC-12, CFC-113, and CFC-114 at 40% in sesame oil were sprayed onto shaved rabbit skin for 12 exposures with no effect.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.73 (1990)]**PEER REVIEWED**

CFC-11, CFC-12, CFC-114, and mixtures of CFC-11 and CFC-12 and of CFC-11 and CFC-22 /were applied/ to the skin, tongue, soft palate and auditory canal of rats, 1-2 times/day, 5 days/week, for 5-6 weeks. The same compounds were applied once a day, 5 days/week for 1 month to the eye of rabbits. Slight irritation was noted only in the skin of the rats and in the eye of the rabbits. The healing rate of experimental burns on the skin of rabbits, however, was noticeably retarded by all of the compounds.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.74 (1990)]**PEER REVIEWED**

Experimental evidence suggests that increased UV-B irradiation at the earth's surface, resulting from ozone depletion /caused by the atmospheric chlorofluorocarbons/, would have deleterious effects on both terrestrial and aquatic biota. Despite uncertanities resulting from the complexities of field experiments, the data currently available suggest that crop yields and forest productivity are vulnerable to increased levels of solar UV-B radiation. Existing data also suggest that increased UV-B radiation will notify the distribution and abundance of plants, and change ecosystem structure. /UV-B Radiation/
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.18 (1990)]**PEER REVIEWED**

Various studies of marine ecosystems have demonstrated that UV-B radiation causes damage to fish larvae and juveniles, shrimp larvae, crab larvae, copepods, and plants essential to the marine food web. These damaging effects include decreased fecundity, growth, and survival. Experimental evidence suggests that even small increases in ambient UV-B exposure could result in significant ecosystem changes. /UV-B Radiation/
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.18 (1990)]**PEER REVIEWED**

Short-term inhalation studies have been reported for CFC-11, CFC-12, CFC-112, CFC-113, CFC-114, and CFC-115. The results showed low toxicity, and the effects observed were related mainly to the CNS, respiratory tract, and the liver. Oral toxicity studies have confirmed the low toxicity.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.18 (1990)]**PEER REVIEWED**

... Dichlorodifluoromethane ... /was/ tested by inhalation on Sprague-Dawley rats and Swiss mice. The animals were exposed for 4 hr a day, 5 days a week; rats were exposed for 104 weeks, and mice were exposed for 78 weeks. Animals were observed until spontaneous death. Exposure of rats to dichlorodifluoromethane resulted in no noticeable differences in the incidence of total benign and malignant tumors, and of the most frequently expected or rate of tumors. Exposure of mice to dichlorodifluoromethane resulted in a higher number of total tumors in males and females which was dose related in males, pulmonary adenomas in males and females at 5000 ppm, and leukemias in males at 5000 and 1000 ppm and in females at 1000 ppm.
[Maltoni C et al; Annals of the New York Academy of Sciences 534: 261-82 (1988)]**PEER REVIEWED**

Groups of four male and four female beagle dogs were orally administered CFC-12 (in frozen dog food) at measured doses of 0, 8, or 80 mg/kg per day for 2 years. None of the dogs died or showed signs of toxicity. No significant differences between treated and control groups were found in food consumption, body weight, periodic hematology, clinical chemistry and urine testing, organ weights, or histopathological findings. An adrenal function test (urinary 17-ketosteroid excretion) also revealed no effects. There was no evidence of carcinogenicity.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.76 (1990)]**PEER REVIEWED**

In a three-generation, oral gavage study in rats using CFC-12 (in corn oil) at average doses of 15 and 150 mg/kg per day. No adverse /were found/ effects on reproductive capability as measured by the fertility index (percentage of matings resulting in pregnancy), gestation index (percentage of pregnancies resulting in birth of live litters), viability index (percentage of rats born that survived four days), and lactation index (percentage of rats alive at 4 days that survived to be weaned at 21 days).
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.77 (1990)]**PEER REVIEWED**

Long term oral studies of CFC-12 /were conducted/ on rats and dogs. As part of a multi-generation reproductive and chronic toxicity study, groups of 50 male and 50 female Charles River rats of the F1a generation remained in the test for 2 years, with an interim kill at 1 year. Starting at 6 weeks of age controls, low dose, and high dose groups were administered CFC-12 in corn oil or corn oil alone daily by gavage for 6 weeks and 5 times per week thereafter. Over the course of the study, actual daily doses of CFC-12 for low dose males and females declined from 27 to 11 and 25 to 11 mg/kg per day respectively and, for the high dose males and females, declined from 273 to 130 and 242 to 128 mg/kg per day, respectively. Average doses were 15 mg/kg per day for the low dose groups and 150 mg/kg per day for the high dose groups. Body weight gain was depressed in the high dose groups, particularly among the females, and a slight decline in food efficiency was noted in high dose females, relative to controls. No overt signs of toxicity were seen, and there were no significant differences between treated and control groups in survival, periodic measurements of hematological, clinical chemistry, and urinalysis values, or in organ weights and histopathological findings. No evidence of carcinogenicity was seen.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.76 (1990)]**PEER REVIEWED**

Groups of 25 to 27 pregnant Charles River rats were given CFC-12 in corn oil by gavage at doses of 16.6 or 179 mg/kg per day on days 6-15 of gestation. Neither dose induced any evidence of embryotoxicity or teratogenicity.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.78 (1990)]**PEER REVIEWED**

Negative results were also obtained for CFC-11, CFC-12, and CFC-115 in a cell transformation assay and for CFC-11 and CFC-12 in a mammalian cell mutagenicity test. CFC-12 was also tested in a plant assay using Tradescantia, and found to be negative.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.79 (1990)]**PEER REVIEWED**

A dominant lethal assay was performed as part of a reproduction study on rats using CFC-12 at doses of 15 and 150 mg/kg per day (gavage) for several weeks. ... Negative results were obtained /in this in vivo assay/ ... .
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.79 (1990)]**PEER REVIEWED**

When administered to groups of 90 male and 90 female /Sprague-Dawley/ rats and 60 male and 60 female /Swiss/ mice at concentrations of 1000 or 5000 ppm (57 or 285 g CFC-11/cu m; 49 or 247 g CFC-12/cu m), 4 hr/day, 5 days/week, neither compound was found to have induced statistically significant differences in the incidence of total benign or malignant tumors when compared with groups of unexposed rats or mice. The incidence of all tumors and of some particularly frequently occurring spontaneous tumors in mice showed a tendency to increase in animals exposed to CFC-11 and CFC-12 and that the increased incidence was usually observed in one sex and was not always dose related, possibly due to a longer survival of the treated mice compared with controls.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.82 (1990)]**PEER REVIEWED**

When the lids of a rabbit's eye have been held open and a blast of a mixture of liquid F-12 and lubricating oil from a refrigeratory has been applied directly to the open eye continously for a second or two, this has caused momentary freezing of the anterior segment of the eye followed by slight epithelial edema and partial loss of epithelium, but complete recovery in three days. Spraying of rabbit eyes with pure liquified F-12 for five to ten seconds caused damage of the corneal endothelium, shedding in gray sheets from the posterior surface of the cornea, and this led to swelling of the stroma. However, there was gradual recovery so that only a small axial nebula persisted after six weeks. Exposure to the liquid spray continuously for thirty seconds caused much more severe corneal damage, the cornea ultimately becoming opaque and the globe /atrophic/ ... .
[Grant, W.M. Toxicology of the Eye. 3rd ed. Springfield, IL: Charles C. Thomas Publisher, 1986. 323]**PEER REVIEWED**

Fluorocarbon inhalation in dogs resulted in dysrhythmias that were enhanced by anoxia, injected epinephrine, and noise stress. Fatal responses resulted from inhaled concentrations of 0.35 to 0.61 per cent of Freon 11 and of 5 percent of Freon 12 and 114.
[Haddad, L.M., Clinical Management of Poisoning and Drug Overdose. 2nd ed. Philadelphia, PA: W.B. Saunders Co., 1990. 1281]**PEER REVIEWED**

EARLY ANIMAL ... WORK INDICATED THAT HIGH VAPOR CONCN (EG, 20%) MAY CAUSE CONFUSION, PULMONARY IRRITATION, TREMORS & RARELY COMA, BUT THAT THESE EFFECTS WERE GENERALLY TRANSIENT & WITHOUT LATE SEQUELAE. /FLUOROCARBON REFRIGERANTS & PROPELLANTS/
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.,p. II-159]**PEER REVIEWED**

The immume system of experimental animals is suppressed in specific ways by UV-B radiation. This results in a decreased resistance to implanted UV-B nduced tumors and an increased growth of such tumors in mice, in the suppression of sensitization by contact allergens, and the response to allergens in sensitized animals. /Chlorofluorocarbons/
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.101 (1990)]**PEER REVIEWED**

Two series of experiments were conducted to determine the effects of bromotrifluoromethane, bromochlorodifluoromethane, and dichlorodifluoromethane on the isolated, perfused rabbit heart. Hearts were perfused through the aorta. Left ventricular mechanical activity was monitored concurrently with action potentials recorded from left ventricular myocardial fibers. In the first series, mechanical performance curves were determined for each of 7 hearts using intraventricular balloons to control end diastolic pressure. The relative effectiveness of these compounds as negative isotropic agents was bromochlorodifluoromethane > dichlorodifluoromethane > bromotrifluoromethane. In the 2nd series of experiments, hearts were exposed to increasing concentrations of each fluorocarbon. Controls were exposed to equivalent concentrations of nitrogen. Exposure to increasing concentrations of bromotrifluoromethane decreased peak left ventricular pressure, the integral of the pressure curve, and the time 20% repolarization of the action potential. Exposure to increasing concentrations of bromochlorodifluoromethane reduced peak left ventricular pressure and the maximal rate of rise of the pressure curve and. Exposure to increasing levels of dichlorodifluoromethane decreased peak left ventricular pressure and the maximal rate of rise of the pressure curve.
[Toy PA, et al; Toxicol Appl Pharmacol 38 (1): 7-18 (1976)]**PEER REVIEWED**

... Chlorofluorocarbons (CFCs) could sensitize the canine myocardium to adrenaline, resulting in serious cardiac arrhythmias.
[Rom, W.N. (ed.). Environmental and Occupational Medicine. 2nd ed. Boston, MA: Little, Brown and Company, 1992. 1300]**PEER REVIEWED**

Three propellant chlorofluorocarbons (FC-11, FC-12 /dichlorodifluoromethane/, FC-22) administered by inhalation at concentrations of 5000 and 1000 ppm for 4 hours daily, 5 days weekly for 78 to 104 weeks to rats and mice had no carcinogenic effects.
[Rom, W.N. (ed.). Environmental and Occupational Medicine. 2nd ed. Boston, MA: Little, Brown and Company, 1992. 1300]**PEER REVIEWED**


Non-Human Toxicity Values:

LD50 Mouse inhalation 760,000 ppm/30 min
[Verschueren, K. Handbook of Environmental Data of Organic Chemicals. 2nd ed. New York, NY: Van Nostrand Reinhold Co., 1983. 485]**PEER REVIEWED**

LD50 Guinea pig inhalation >800,000 ppm/30 min
[Verschueren, K. Handbook of Environmental Data of Organic Chemicals. 2nd ed. New York, NY: Van Nostrand Reinhold Co., 1983. 485]**PEER REVIEWED**

LD50 Rabbit inhalation >800,000 ppm/30 min
[Verschueren, K. Handbook of Environmental Data of Organic Chemicals. 2nd ed. New York, NY: Van Nostrand Reinhold Co., 1983. 485]**PEER REVIEWED**

LD50 Rat single oral >1 g/kg
[Verschueren, K. Handbook of Environmental Data of Organic Chemicals. 2nd ed. New York, NY: Van Nostrand Reinhold Co., 1983. 485]**PEER REVIEWED**

LD50 Rat inhalation >800,000 ppm/30 min
[Verschueren, K. Handbook of Environmental Data of Organic Chemicals. 2nd ed. New York, NY: Van Nostrand Reinhold Co., 1983. 485]**PEER REVIEWED**


Metabolism/Pharmacokinetics:

Absorption, Distribution & Excretion:

ELIMINATION IS RAPID. DOGS EXHALED WITHIN 1 HR ESSENTIALLY ALL FC 12 INHALED DURING 6-20 MIN EXPOSURE TO 8000-12000 PPM. INHALED FC 12 RAPIDLY APPEARED IN BLOOD, BILE, CEREBROSPINAL FLUID & URINE OF ANESTHETIZED RABBITS & DOGS. UNANESTHETIZED DOGS EXPOSED TO 1000-100000 PPM FOR 10 MIN SHOWED RAPID RISE IN BLOOD CONCN ... DURING 1ST 3 TO 5 MIN WHICH WAS PARALLELED BY A RAPID DECLINE FOR FIRST 5 MIN AFTER EXPOSURE.
[American Conference of Governmental Industrial Hygienists. Documentation of the Threshold Limit Values and Biological Exposure Indices. 5th ed. Cincinnati, OH:American Conference of Governmental Industrial Hygienists, 1986. 181]**PEER REVIEWED**

BLOOD LEVELS OF FC 12 WERE BELOW DETECTION LIMITS IN NORMAL SUBJECTS USING HOUSEHOLD AEROSOLS; IN ASTHMATIC SUBJECTS USING AEROSOL INHALER, BLOOD LEVELS WERE MUCH LOWER THAN IN DOGS EXPOSED AT THRESHOLD FOR CARDIAC SENSITIZATION. RADIOLABELED TESTS SHOWED ESSENTIALLY ALL DOSE (95-103%) EXHALED WITHIN 1ST HR AFTER 12 OR 17 MIN INHALATION AT 1000 PPM. ... FOR AN EIGHT HR INHALATION AT 1000 PPM, A PHARMACOKINETIC MODEL BASED ON ANALYSES IN DOGS AND HUMANS GAVE AN ESTIMATE OF 55% ABSORPTION OF THE INHALED FC 12.
[American Conference of Governmental Industrial Hygienists. Documentation of the Threshold Limit Values and Biological Exposure Indices. 5th ed. Cincinnati, OH:American Conference of Governmental Industrial Hygienists, 1986. 181]**PEER REVIEWED**

AT 1000 PPM ... LEVEL IN /HUMAN/ VENOUS BLOOD WAS 1.2 UG/ML.
[National Research Council. Drinking Water and Health. Volume 3. Washington, DC: National Academy Press, 1980. 101]**PEER REVIEWED**

Absorption and elimination are dynamic processes involving equilibria among air, blood, and various tissues. Upon absorption, a biphasic blood-level pattern occurs, with an initial rapid then slower rise in blood levels, during which the material is absorbed from blood into tissue.
[USEPA; Ambient Water Quality Criteria Doc: Halomethanes p.C-26 (1980) EPA 440/5-80-051]**PEER REVIEWED**

Human & animal studies indicate rapid excretion of inhaled FC-114. In a study with radiolabeled FC-114, 30 min retention of the dose inhaled in a single breath was 12% versus 23%, 10%, & 20% for comparable doses of FC-11, FC-12, and FC-113, respectively.
[American Conference of Governmental Industrial Hygienists. Documentation of the Threshold Limit Values and Biological Exposure Indices. 5th ed. Cincinnati, OH:American Conference of Governmental Industrial Hygienists, 1986. 191]**PEER REVIEWED**

... MAIN FACTOR AFFECTING FATE OF FLUOROCARBONS IS BODY FAT, WHERE THEY ARE CONCENTRATED & SLOWLY RELEASED INTO BLOOD @ CONCN THAT SHOULD NOT CAUSE ANY RISK OF CARDIAC SENSITIZATION. /FLUOROCARBONS/
[National Research Council. Drinking Water & Health Volume 1. Washington, DC: National Academy Press, 1977. 781]**PEER REVIEWED**

There is a significant accumulation of fluorocarbons in brain, liver and lung compared to blood levels, signifying a tissue distribution of fluorocarbons similar to that of chloroform. /Fluorocarbons/
[Clayton, G.D., F.E. Clayton (eds.) Patty's Industrial Hygiene and Toxicology. Volumes 2A, 2B, 2C, 2D, 2E, 2F: Toxicology. 4th ed. New York, NY: John Wiley & Sons Inc., 1993-1994. 1203]**PEER REVIEWED**

Abosrption of fluorocarbons is much lower after oral ingestion (35-48 times) than after inhalation. ... The lung generally has the highest fluorocarbon concentrations on autopsy. /Fluorocarbons/
[Ellenhorn, M.J. and D.G. Barceloux. Medical Toxicology - Diagnosis and Treatment of Human Poisoning. New York, NY: Elsevier Science Publishing Co., Inc. 1988. 884]**PEER REVIEWED**

Although fluorocarbons cause cardiac sensitization in certain animal species, rapid elimination prevents the development of cardiotoxic concentrations from aerosol bronchodilator use except at exceedingly high doses (12 to 24 doses in 2 minutes). /Fluorocarbons/
[Ellenhorn, M.J. and D.G. Barceloux. Medical Toxicology - Diagnosis and Treatment of Human Poisoning. New York, NY: Elsevier Science Publishing Co., Inc. 1988. 884]**PEER REVIEWED**

FLUOROCARBON COMPOUNDS ARE LIPID-SOLUBLE AND THUS ARE GENERALLY WELL ABSORBED THROUGH LUNG. ABSORPTION AFTER INGESTION IS 35 TO 48 TIMES LOWER THAN AFTER INHALATION. ... FLUOROCARBONS ARE ELIMINATED BY WAY OF LUNG. /FLUOROCARBON COMPOUNDS/
[National Research Council. Drinking Water and Health. Volume 3. Washington, DC: National Academy Press, 1980. 101]**PEER REVIEWED**

Regardless of the route of entry, chlorofluorocarbons appear to be eliminated almost exclusively through the respiratory tract. Little, if any, chlorofluorocarbon or metabolite has ever been reported in urine or feces. /Chlorofluorocarbons/
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.60 (1990)]**PEER REVIEWED**


Biological Half-Life:

The distribution half-life of the common fluorocarbons (Freon 11, Freon 12) averages 13 to 14 seconds; the elimination half-life is longer (1.5 hours) because of slower release from fat stores.
[Ellenhorn, M.J. and D.G. Barceloux. Medical Toxicology - Diagnosis and Treatment of Human Poisoning. New York, NY: Elsevier Science Publishing Co., Inc. 1988. 884]**PEER REVIEWED**


Mechanism of Action:

On the basis of an electrophysiological analysis of the action of CFC-12 on different types of cardiac cells from rats and sheep, the authors conclude that: 1. the cardiac depression observed during inhalation of CFC-12 (and many other volatile liposoluble compounds) is the consequence of a non-specific impairment of the membrane properties and notably the inhibition of trans-membrane ionic currents; 2, CFC-12 action on ionic currents is variable: at high concentrations, depending on the type of cardiac cell, it can oppose or favor the action of adrenaline, giving rise to many factors that lead to arrhythmia.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.87 (1990)]**PEER REVIEWED**

CFC-12 and CFC-114 do not markedly affect oxygen consumption or oxidative phosphorylation in mitochondria isolated from the liver, lung, brain, heart, or kidney of rats exposed to about 7.5% chlorofluorocarbons prior to mitochondrial isolation. Further in vitro studies were conducted with liver and heart mitochondria in which measurements were taken during exposure of the mitochondria to CFC-12 at 990 g/cu m (20%) (time of exposure not specified). No effects on either oxidation or phosphorylation were noted.
[WHO; Environmental Health Criteria 113: Fully Halogenated Chlorofluorocarbons p.87 (1990)]**PEER REVIEWED**


Interactions:

AFTER ACUTE EXPOSURE BY INHALATION TO A 13.5% CONCENTRATION FOR 30 SEC, MYOCARDIUM IN UNANESTHETIZED DOGS WAS SENSITIZED TO SUBSEQUENT INJECTION OF EPINEPHRINE. IN CONTRAST, A 2.5% CONCENTRATION THAT WAS INHALED 6 HR/DAY FOR 5 DAYS RESULTED IN NO CARDIAC SENSITIZATION IN DOGS.
[National Research Council. Drinking Water and Health. Volume 3. Washington, DC: National Academy Press, 1980. 102]**PEER REVIEWED**

/IN HUMANS/ A 10 TO 90% MIXTURE OF CFC 11 & CFC 12, RESPECTIVELY, CAUSED MORE SEVERE RESPIRATORY EFFECTS THAN EITHER FLUOROCARBON INHALED SINGLY.
[Clayton, G.D., F.E. Clayton (eds.) Patty's Industrial Hygiene and Toxicology. Volumes 2A, 2B, 2C, 2D, 2E, 2F: Toxicology. 4th ed. New York, NY: John Wiley & Sons Inc., 1993-1994. 1201]**PEER REVIEWED**

IF INHALATION OCCURS, EPINEPHRINE OR OTHER SYMPATHOMIMETIC AMINES & ADRENERGIC ACTIVATORS SHOULD NOT BE ADMIN SINCE THEY WILL FURTHER SENSITIZE HEART TO DEVELOPMENT OF ARRHYTHMIAS. /FLUOROCARBONS/
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 3101]**PEER REVIEWED**


Pharmacology:

Interactions:

AFTER ACUTE EXPOSURE BY INHALATION TO A 13.5% CONCENTRATION FOR 30 SEC, MYOCARDIUM IN UNANESTHETIZED DOGS WAS SENSITIZED TO SUBSEQUENT INJECTION OF EPINEPHRINE. IN CONTRAST, A 2.5% CONCENTRATION THAT WAS INHALED 6 HR/DAY FOR 5 DAYS RESULTED IN NO CARDIAC SENSITIZATION IN DOGS.
[National Research Council. Drinking Water and Health. Volume 3. Washington, DC: National Academy Press, 1980. 102]**PEER REVIEWED**

/IN HUMANS/ A 10 TO 90% MIXTURE OF CFC 11 & CFC 12, RESPECTIVELY, CAUSED MORE SEVERE RESPIRATORY EFFECTS THAN EITHER FLUOROCARBON INHALED SINGLY.
[Clayton, G.D., F.E. Clayton (eds.) Patty's Industrial Hygiene and Toxicology. Volumes 2A, 2B, 2C, 2D, 2E, 2F: Toxicology. 4th ed. New York, NY: John Wiley & Sons Inc., 1993-1994. 1201]**PEER REVIEWED**

IF INHALATION OCCURS, EPINEPHRINE OR OTHER SYMPATHOMIMETIC AMINES & ADRENERGIC ACTIVATORS SHOULD NOT BE ADMIN SINCE THEY WILL FURTHER SENSITIZE HEART TO DEVELOPMENT OF ARRHYTHMIAS. /FLUOROCARBONS/
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 3101]**PEER REVIEWED**


Environmental Fate & Exposure:

Environmental Fate/Exposure Summary:

Dichlorodifluoromethane's former production and use as an aerosol propellent, foaming agent and refrigerant lead to its release to the environment through various waste streams. Based on a vapor pressure of 4,850 mm Hg at 25 deg C, dichlorodifluoromethane is expected to exist solely in the gas-phase in the ambient atmosphere. Gas-phase dichlorodifluoromethane is extremely stable in the troposphere. This compound does not react with photochemically produced hydroxyl radicals, ozone molecules or nitrate radicals in the troposphere. This compound will gradually diffuse into the stratosphere above the ozone layer where it will slowly degrade due to direct photolysis from UV-C radiation and contribute to the catalytic removal of stratospheric ozone. Dichlorodifluoromethane is expected to have moderate mobility in soils based upon an estimated Koc value of 356. This compound is expected to volatilize rapidly from dry soil surfaces based on its measured vapor pressure. Volatilization from moist soil surfaces is expected based upon a Henry's Law constant of 0.343 atm-cu m/mole. Dichlorodifluoromethane was degraded under anaerobic conditions in laboratory tests, but does not biodegrade under aerobic conditions. In water, dichlorodifluoromethane is not expected to adsorb to sediment or particulate matter given its estimated Koc value. This compound is expected to volatilize rapidly from water surfaces given its Henry's Law constant. Estimated half-lives for a model river and model lake are 1 hour and 4 days, respectively. An estimated BCF of 25 suggests the potential for bioconcentration in aquatic organisms is low. Occupational exposure may be through inhalation and dermal contact with this compound at workplaces where dichlorodifluoromethane is still used, such as air condition repair shops. However, since this compound is no longer produced in the US, very little occupational exposure is expected. Due to its long atmospheric residence time, the general population is exposed to dichlorodifluoromethane through inhalation of ambient air. (SRC)
**PEER REVIEWED**


Probable Routes of Human Exposure:

NIOSH (NOES Survey 1981-1983) has statistically estimated that 435,098 workers (125,602 of these are female) are potentially exposed to dichlorodifluoromethane in the US(1). Occupational exposure may be through inhalation and dermal contact with this compound at workplaces where dichlorodifluoromethane is still used, such as air conditioning repair shops(SRC). This survey was conducted prior to the Montreal Protocol which scheduled the production phase-out of this compound and other chlorofluorocarbons, and is not an accurate measure of the current occupational exposure(SRC). Due to its long atmospheric residence time, the general population may be exposed to dichlorodifluoromethane via inhalation of ambient air(SRC).
[(1) NIOSH; National Occupational Exposure Survey (NOES) (1983)]**PEER REVIEWED**


Body Burden:

In a pilot study of pollutants in the milk of women living in 4 urban-industrial areas in the US, dichlorodifluoromethane was identified, not quantified, in 2 of 8 samples(1).
[(1) Pellizzari ED et al; Bull Environ Contam Toxicol 28: 322-8 (1982)]**PEER REVIEWED**


Natural Pollution Sources:

There are no known natural sources of dichlorodifluoromethane(1).
[(1) Bullister JL, Weiss RF; Science 221: 265-8 (1983)]**PEER REVIEWED**


Artificial Pollution Sources:

All of the dichlorodifluoromethane that is produced is eventually lost as emissions. It is estimated that 3.3% of the dichlorodifluoromethane produced is lost from plant vents and during packaging, a loss which is immediate(1). Losses from aerosols occur, on the average, 6 months after sale; losses from domestic refrigerators and freezers have an average life of 12 yr with a 2% loss during filling; industrial refrigerator charges have an average lifetime of 4 yr; all of the dichlorodifluoromethane used in closed cell foams are lost within 2 yr with 75% being lost during the first year(1). The annual world production and release of dichlorodifluoromethane in 1982 was 443.7 and 422.8 million kg, respectively(1). Cumulative production and release estimates up until the end of 1974 were 4698.5 and 4286.2 million kg(1). By the end of 1982 these figures had increased to 8196.0 and 7520.2 million kg(1).
[(1) Gamlen PH et al; Atmos Environ 20: 1077-85 (1986)]**PEER REVIEWED**

The realization that certain chlorofluorocarbons can accumulate in the upper atmosphere and deplete the earth's ozone layer has had a major impact on chemicals like dichlorodifluoromethane which are used in large quantities and have the stability to reach the stratosphere. Uses such as propellants in aerosols which had accounted for about 75% of the release of dichlorodifluoromethane and trichlorofluoromethane, the chemicals of greatest concern (refrigerants and foams accounted for about 14 and 12%, respectively), were banned in the US after Dec 15, 1978(1). Previously dichlorodifluoromethane was the principal propellant for non-food aerosols(1) and 60% of dichlorodifluoromethane and trichlorofluoromethane production went into aerosols(1).
[(1) Smart BE; Kirk Othmer's Encycl Chem Tech 3rd NY,NY: Wiley Interscience 10: 829-70 (1980)]**PEER REVIEWED**

Dichlorodifluoromethane's former production and use as an aerosol propellent, foaming agent and refrigerant(1) lead to its release to the environment through various waste streams(SRC).
[(1) Sibley HW; Kirk-Othmer Encycl Chem Technol. 4th ed. NY, NY: Wiley 21: 128-49 (1997)]**PEER REVIEWED**


Environmental Fate:

Due to the high vapor pressure of dichlorodifluoromethane, volatilization to the atmosphere is quite rapid. ... It does not react readily with hydroxyl radicals, nor does it photodissociate in the troposphere since it exhibits no absorption of light greater than 200 nm. ... In the stratosphere, dichlorodifluoromethane is broken down by the absorption of higher energy, shorter wavelength ultraviolet light.
[Callahan, M.A., M.W. Slimak, N.W. Gabel, et al. Water-Related Environmental Fate of 129 Priority Pollutants. Volume I. EPA-440/4 79-029a. Washington, DC: U.S.Environmental Protection Agency, December 1979.,p. 62-2]**PEER REVIEWED**

TERRESTRIAL FATE: Based on a recommended classification scheme(1), an estimated Koc value of 356(SRC), determined from a log Kow of 2.16(2) and a recommended regression-derived equation(3), indicates that dichlorodifluoromethane is expected to have moderate mobility in soil(SRC). Volatilization of dichlorodifluoromethane is expected from moist soil surfaces(SRC) given a Henry's Law constant of 0.343 atm-cu m/mole(4). Dichlorodifluoromethane is expected to volatilize rapidly from dry soil surfaces(SRC) based on a vapor pressure of 4,850 mm Hg at 25 deg C(5). Dichlorodifluoromethane was degraded under anaerobic landfill conditions in laboratory test digesters(6), suggesting this compound may biodegrade in soils under anaerobic conditions(SRC).
[(1) Swann RL et al; Res Rev 85: 23 (1983) (2) Hansch C, Leo A; Exploring QSAR Fundamentals and Applications in Chemistry and Biology. Washington, DC: Amer Chem Soc p. 3 (1995) (3) Lyman WJ et al; Handbook of Chemical Property Estimation Methods. Washington DC: Amer Chem Soc pp. 4-9 (1990) (4) Warner MJ, Weiss RF; Deep Sea Res 32A: 1485-97 (1985) (5) Daubert TE, Danner RP; Physical and Thermodynamic Properties of Pure Chemicals Data Compilation Washington, DC: Taylor and Francis (1989) (6) Deipser A, Stegmann R; Environ Sci Pollut Res 4: 209-16 (1997)]**PEER REVIEWED**

AQUATIC FATE: Based on a recommended classification scheme(1), an estimated Koc value of 356(SRC), determined from a log Kow of 2.16(2) and a recommended regression-derived equation(3), indicates that dichlorodifluoromethane is not expected to adsorb to suspended solids and sediment in water(SRC). Dichlorodifluoromethane is expected to volatilize rapidly from water surfaces(3) based on a Henry's Law constant of 0.343 atm-cu m/mole(4). Estimated half-lives for a model river and model lake are 1 hour and 4 days, respectively(SRC). According to a classification scheme(5), an estimated BCF value of 25(SRC), determined from the log Kow(2), and a regression-derived equation(3), suggests the potential for bioconcentration in aquatic organisms is low(SRC). Dichlorodifluoromethane was degraded under anaerobic conditions over a 100 day incubation period by sediment obtained from the Potomac River(6).
[(1) Swann RL et al; Res Rev 85: 23 (1983) (2) Hansch C, Leo A; Exploring QSAR Fundamentals and Applications in Chemistry and Biology. Washington, DC: Amer Chem Soc p. 3 (1995) (3) Lyman WJ et al; Handbook of Chemical Property Estimation Methods. Washington, DC: Amer Chem Soc pp. 4-9, 5-4, 5-10, 15-1 to 15-29 (1990) (4) Warner MJ, Weiss RF; Deep Sea Res 32A: 1485-97 (1985) (5) Franke C et al; Chemosphere 29: 1501-14 (1994) (6) Lovely DR, Woodward JC; Environ Sci Technol 26: 925-29 (1992)]**PEER REVIEWED**

ATMOSPHERIC FATE: According to a model of gas/particle partitioning of semivolatile organic compounds in the atmosphere(1), dichlorodifluoromethane, which has a vapor pressure of 4,850 mm Hg at 25 deg C(2), is expected to exist solely as a gas in the ambient atmosphere. Gas-phase dichlorodifluoromethane is extremely stable in the troposphere. This compound does not react with photochemically produced hydroxyl radicals, ozone molecules or nitrate radicals(3,4). This compound will gradually diffuse into the stratosphere above the ozone layer where it will slowly degrade due to direct photolysis from UV-C radiation and contribute to the catalytic removal of stratospheric ozone(SRC). The half-life for this reaction has been estimated to range from 105 to 169 years(4).
[(1) Bidleman TF; Environ Sci Technol 22: 361-367 (1988) (2) Daubert TE, Danner RP; Physical and Thermodynamic Properties of Pure Chemicals Data Compilation Washington, DC: Taylor and Francis (1989) (3) Atkinson R; J Phys and Chem Ref Data Monograph 1: 66 (1989) (4) Fabian P, Gomer D; Fresenius Z Anal Chem 319: 890-97 (1984)]**PEER REVIEWED**


Environmental Biodegradation:

AEROBIC: No evidence of dichlorodifluoromethane biodegradation was found in a microcosm designed to simulate Narragansett Bay in a month-long experiment(1). ANAEROBIC: Pure cultures of Methanosarcina barkerii and Methanobacterium thermoautotrophicum were shown to biodegrade dichlorodifluoromethane via reductive dehalogenation(2). Pure bacterial cultures isolated from soils obtained from Rockville, MD were shown to biodegrade dichlorodifluoromethane(4). Dichlorodifluoromethane was degraded under anaerobic landfill conditions in laboratory test digesters(3). The degradation product observed was dichlorofluoromethane(3). Dichlorodifluoromethane was degraded under anaerobic conditions over a 100 day incubation period by sediment from the Potomac River(4), but was not biodegraded by an aerobic soil.
[(1) Bopp RF et al; Org Geochem 3: 9-14 (1981) (2) Sylvestre M et al; Crit Rev Environ Sci Technol 27: 87-111 (1997) (3) Deipser A, Stegmann R; Environ Sci Pollut Res 4: 209-16 (1997) (4) Lovely DR, Woodward JC; Environ Sci Technol 26: 925-29 (1992)]**PEER REVIEWED**


Environmental Abiotic Degradation:

... Photolysis of dichlorodifluoromethane in the presence of oxygen at 213.9 nm and 25 deg C, leads to the prodn of difluoromethoxide and chloride ... and chlorine atoms.
[Callahan, M.A., M.W. Slimak, N.W. Gabel, et al. Water-Related Environmental Fate of 129 Priority Pollutants. Volume I. EPA-440/4 79-029a. Washington, DC: U.S.Environmental Protection Agency, December 1979.,p. 62-3]**PEER REVIEWED**

... PHOTODISSOCIATION OF FLUOROCARBONS IN STRATOSPHERE PRODUCES SIGNIFICANT AMT OF CHLORINE ATOMS /SRP: FLUORINE ATOMS AND BROMINE ATOMS/ & LEADS TO DESTRUCTION OF ATMOSPHERIC OZONE. REDUCTION IN OZONE ALLOWS MORE UV LIGHT TO REACH EARTH'S SURFACE ... /FLUOROCARBONS/
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 3090]**PEER REVIEWED**

Gas-phase dichlorodifluoromethane is extremely stable in the troposphere. This compound does not react with photochemically produced hydroxyl radicals, ozone molecules or nitrate radicals(1,2). This compound will gradually diffuse into the stratosphere above the ozone layer where it will slowly degrade due to direct photolysis from UV-C radiation and contribute to the catalytic removal of stratospheric ozone(2). The half-life for this reaction has been estimated to range from 105 to 169 years(2). Dichlorodifluoromethane is not expected to undergo hydrolysis or direct photolysis in the troposphere due to the lack of functional groups that could chemically hydrolyze or absorb light at environmentally significant wavelengths(3).
[(1) Atkinson R; J Phys and Chem Ref Data Monograph 1: 66 (1989) (2) Fabian P, Gomer D; Fresenius Z Anal Chem 319: 890-97 (1984) (3) Lyman WJ et al; Handbook of Chemical Property Estimation Methods. Washington, DC: Amer Chem Soc pp. 7-4, 7-5 (1990)]**PEER REVIEWED**


Environmental Bioconcentration:

... The log octanol/water partition coefficient (log P) of 2.16 indicates that dichlorodifluoromethane is lipophilic and that bioaccumulation in organisms may be possible under conditions of constant exposure.
[Callahan, M.A., M.W. Slimak, N.W. Gabel, et al. Water-Related Environmental Fate of 129 Priority Pollutants. Volume I. EPA-440/4 79-029a. Washington, DC: U.S.Environmental Protection Agency, December 1979.,p. 62-4]**PEER REVIEWED**

An estimated BCF value of 25 was calculated for dichlorodifluoromethane(SRC), using a log Kow of 2.16(1) and a recommended regression-derived equation(2). According to a classification scheme(3), this BCF value suggests that the potential bioconcentration in aquatic organisms is low(SRC).
[(1) Hansch C, Leo A; Exploring QSAR Fundamentals and Applications in Chemistry and Biology. Washington, DC: Amer Chem Soc p. 3 (1995) (2) Lyman WJ et al; Handbook of Chemical Property Estimation Methods. Washington DC: Amer Chem Soc pp. 5-4, 5-10 (1990) (3) Franke C et al; Chemosphere 29: 1501-14 (1994)]**PEER REVIEWED**


Soil Adsorption/Mobility:

... The log octanol/water partition coefficient (log P) of 2.16 ... indicates that absorption onto organic particulates may be possible.
[Callahan, M.A., M.W. Slimak, N.W. Gabel, et al. Water-Related Environmental Fate of 129 Priority Pollutants. Volume I. EPA-440/4 79-029a. Washington, DC: U.S.Environmental Protection Agency, December 1979.,p. 62-4]**PEER REVIEWED**

The Koc of dichlorodifluoromethane is estimated as approximately 356(SRC), using a log Kow of 2.16(1) and a regression-derived equation(2). According to a recommended classification scheme(3), this estimated Koc value suggests that dichlorodifluoromethane is expected to have moderate mobility in soil(SRC).
[(1) Hansch C, Leo A; Exploring QSAR Fundamentals and Applications in Chemistry and Biology. Washington, DC: Amer Chem Soc p. 3 (1995) (2) Lyman WJ et al; Handbook of Chemical Property Estimation Methods. Washington, DC: Amer Chem Soc pp. 4-9 (1990) (3) Swann RL et al; Res Rev 85: 23 (1983)]**PEER REVIEWED**


Volatilization from Water/Soil:

The high vapor pressure and low aqueous solubility of dichlorodifluoromethane are conductive to rapid volatilization from an aquatic environment. ... Volatilization is thought to be the major transport process.
[Callahan, M.A., M.W. Slimak, N.W. Gabel, et al. Water-Related Environmental Fate of 129 Priority Pollutants. Volume I. EPA-440/4 79-029a. Washington, DC: U.S.Environmental Protection Agency, December 1979.,p. 62-4]**PEER REVIEWED**

The Henry's Law constant for dichlorodifluoromethane is 0.343 atm-cu m/mole(1). This value indicates that dichlorodifluoromethane will volatilize rapidly from water surfaces(2). Based on this Henry's Law constant, the volatilization half-life from a model river (1 m deep, flowing 1 m/sec, wind velocity of 3 m/sec)(2) is estimated as approximately 1 hour(SRC). The volatilization half-life from a model lake (1 m deep, flowing 0.05 m/sec, wind velocity of 0.5 m/sec)(2) is estimated as approximately 4 days(SRC). Dichlorodifluoromethane's Henry's Law constant(1) indicates that volatilization from moist soil surfaces is expected occur(SRC). Dichlorodifluoromethane is expected to volatilize rapidly from dry soil surfaces(SRC) based on the vapor pressure of 4,850 mm Hg at 25 deg C(3).
[(1) Warner MJ, Weiss RF; Deep Sea Res 32A: 1485-97 (1985) (2) Lyman WJ et al; Handbook of Chemical Property Estimation Methods. Washington DC: Amer Chem Soc pp. 15-1 to 15-29 (1990) (3) Daubert TE, Danner RP; Physical and Thermodynamic Properties of Pure Chemicals Data Compilation Washington, DC: Taylor and Francis (1989)]**PEER REVIEWED**


Environmental Water Concentrations:

GROUNDWATER: Dichlorodifluoromethane was identified, but not quantified in groundwater under 6 of 13 municipal landfills in Minnesota with suspected leakage but not in the groundwater under 7 other municipal landfills(1). Dichlorodifluoromethane was detected in 20 of 2,542 groundwater rural wells at a max concn of about 7 ug/l and in 11 out of 406 urban wells at a max concn of about 70 ug/l in the US from 1985-1995(2). Dichlorodifluoromethane was detected in shallow wells in northern Hungary at concns of 0.06-10.36 picomols/l in 1993(3). Dichlorodifluoromethane was detected at a max concn of 0.6 ug/l in groundwater from Denver, CO(4).
[(1) Sabel GV, Clark TP; Waste Manag Res 2: 119-30 (1984) (2) Squillace PJ et al; Environ Sci Technol 33: 4176-87 (1999) (3) Bohlke JK et al; Environ Sci Technol 31: 3293-99 (1997) (4) Bruce BW et al; J Hydrol 186: 129-51 (1996)]**PEER REVIEWED**

SURFACE WATER: Of the 696 stations reporting pollutants in ambient waters in EPA's STORET database, 1.0% contained dichlorodifluoromethane at detectable levels(1). Dichlorodifluoromethane was identified, but not quantified, in the open waters of Lake Erie(3). The average concn of dichlorodifluoromethane in surface water at two stations in the Greenland and Norwegian Sea was 218 and 187 ppb, respectively(2). The concn decreased with depth over many km in the Greenland Sea and was relatively constant in the upper 0.4 km in the Norwegian Sea before decreasing(2). The deep water concns of dichlorodifluoromethane in these seas were 37 and 18 ppb(2).
[(1) Staples CA et al; Environ Toxicol Chem 4: 131-42 (1985) (2) Bullister JL, Weiss RF; Science 221: 265-8 (1983) (3) Great Lakes Water Quality Board; Report to the Great Lake Water Quality Board Windsor Ontario Canada pp. 195 (1983)]**PEER REVIEWED**


Effluent Concentrations:

Of the 1,144 stations reporting pollutants in ambient waters in EPA's STORET database, 1.6% contained dichlorodifluoromethane at detectable levels(1). No dichlorodifluoromethane was found in the effluent of a large community septic tank (detection limit 0.7 ppb)(2). In the National Urban Runoff Program in which samples of runoff were collected from 19 cities (51 catchments) in the U.S., no dichlorodifluoromethane was found in any samples(3). Leachate from 2 of 6 municipal landfills tested in Minnesota had detectable quantities of dichlorodifluoromethane(4). Leachate from 1 out of 5 landfills in Wisconsin contained 180 ppb of the chemical(4). Dichlorodifluoromethane was emitted from a simulated landfill composed of municipal refuse and wastewater sludges(5).
[(1) Staples CA et al; Environ Toxicol Chem 4: 131-42 (1985) (2) Dewalle FB et al; Determination of Toxic Chemicals in Effluent from Household Septic Tanks USEPA-600/S2-85-050. Cincinnati OH: p. 4 (1985) (3) Cole RH et al; J Water Pollut Control Fed 56: 898-908 (1984) (4) Sabel GV, Clark TP; Waste Manag Res 2: 119-30 (1984) (5) Vogt WG, Walsh JJ; in Proc - APCA Annu Meet 78th p. 17 (1985)]**PEER REVIEWED**


Sediment/Soil Concentrations:

None of the 204 stations in EPA's STORET database reported any dichlorodifluoromethane in sediment(1).
[(1) Staples CA et al; Environ Toxicol Chem 4: 131-42 (1985)]**PEER REVIEWED**


Atmospheric Concentrations:

Mean urban concn for dichlorodifluoromethane ranged from 3.5x10-3 to 2.9x10-2 mg/cu m, and an ocean atmosphere mean of 2.7x10-4 mg/cu m.
[USEPA; Ambient Water Quality Criteria Doc: Halomethanes p.C-17 (1980) EPA 440/5-80-051]**PEER REVIEWED**

In a beauty shop, where fluorocarbon pressured cosmetic sprays were apt to be used, concn of 1.8 mg/cu m.
[USEPA; Ambient Water Quality Criteria Doc: Halomethanes p.C-18 (1980) EPA 440/5-80-051]**PEER REVIEWED**

Dichlorodifluoromethane concn in room air as a result of release of aerosol can products. Level at periods after 60 sec release of hair spray in a 29.3 cu m room (in mg/cu m): during release: 306.8; 30 min after: 12.4; 60 min after: 0.5. Level at periods after 30 second release of insect spray in a 21.4 cu m room (in mg/cu m): 1 min: 2,304.0; 60 min: 130.4; 150 min: 56.8. /From table/
[USEPA; Ambient Water Quality Criteria Doc: Halomethanes p.C-19 (1980) EPA 440/5-80-051]**PEER REVIEWED**

Ambient air quality, ground level concn in Los Angeles; July 1970, 0.7 ppb, Feb 1973, 0.53 ppb; in rural Wash DC; Dec 74-Feb 75: 0.23 ppb.
[Verschueren, K. Handbook of Environmental Data on Organic Chemicals. 3rd ed. New York, NY: Van Nostrand Reinhold Co., 1996. 682]**PEER REVIEWED**

S Calif Feb-Aug 1973 at 6700 m, 90 parts per trillion ; New Mexico on 5/23/74 at 6400 m 120 parts per trillion; W Ireland June/July 1974 at sea-level, 101.7 parts per trillion; North Atlantic Oct 1974 at sea-level, 115 parts per trillion; Pullman, Washington Nov 1974 at 0-3600 m, 230 parts per trillion; West of N Ireland E of Scotland at 7000-8000 m, 231 parts per trillion; off SW Wales Jan 1976 at 900-7300 m, 236 parts per trillion; off SW Wales Jan 1976 at 900-4900 m, 258 parts per trillion; off NE England Feb 1976 at 400-5200 m, 225 parts per trillion /From table/
[Verschueren, K. Handbook of Environmental Data on Organic Chemicals. 3rd ed. New York, NY: Van Nostrand Reinhold Co., 1996. 682]**PEER REVIEWED**

RURAL/REMOTE: Sites in the US (431 samples) - 120 parts per trillion, median, 230 parts per trillion, max(1). The average concn of dichlorodifluoromethane at two stations over the Greenland and Norwegian Sea was 343 parts per trillion(2). The concn of dichlorofluoromethane in Adrigole, Ireland was 338 parts per trillion(2). Rural and urban areas of China and rural areas of Oregon contained a median concn of dichlorofluoromethane was 320 parts per trillion(3). Similarly, median concns of dichlorodifluoromethane in rural areas of Eastern Washington and the Caucasus Mountains of the USSR in 1979 was 289 and 313 parts per trillion, respectively(4). The respective average concns in the morning vs the nighttime in Eastern Washington was 310 and 318 parts per trillion(4). The background concn of dichlorodifluoromethane in the northern hemisphere was reported as 530 parts per trillion(5).
[(1) Brodzinsky R, Singh HB; Volatile Organic Chemicals in the Atmosphere: An Assessnebt of Available Data Menlo Park CA: SRI Inter Contract 68-02-3452 (1982) (2) Bullister JL, Weiss RF; Science. 221: 265-8 (1983) (3) Rasmussen RA et al; Environ Sci Tech 16: 124-6 (1982) (4) Cronn DR et al; Environ Sci Tech 17: 383-8 (1983) (5) Wang JL et al; Chemosphere 36: 2391-2400 (1998)]**PEER REVIEWED**

RURAL/REMOTE: The average concns of dichlorodifluoromethane in the Northern and Southern Hemispheres show slight differences(3). In 1976, their values were 218 and 204 parts per trillion(3), respectively. Concns of dichlorodifluoromethane at the South Pole has increased from 195 to 284 parts per trillion between 1976 and 1980, while over the same period of time the increase in the Pacific Northwest was from 228 to 322 parts per trillion(1). The annual rate of increase in these areas is 7%. As the altitude increases, the concn of dichlorodifluoromethane in the atmosphere at northern mid-latitudes decreased from 350 parts per trillion at the earth's surface to 250, 100, and 20 parts per trillion at 10, 20, and 30 km(2). The avg concn of dichlorodifluoromethane in coastal regions of Taiwan was 515 parts per trillion(4).
[(1) Crutzen PJ, Gidel LT; J Geophys Res 88: 6641-61 (1983) (2) Fabian P, Goemer D; Fresenius' Z Anal Chem 319: 890-7 (1984) (3) Tyson BJ et al; Geophys Res Lett 5: 535-8 (1978) (4) Wang JL et al; Chemosphere 36: 2391-2400 (1998)]**PEER REVIEWED**

URBAN/SUBURBAN: Dichlorodifluoromethane was detected at various sites in the US at a median concn of 0.38 ppb and a max concn of 4.9 ppb(1). Various sites in the US had dichlorodifluoromethane levels ranging from 0.37 to 4.8 ppb while sites in continental Europe and Japan had levels of 0.410-11.4 ppb(2). In 1975, the average concn of dichlorodifluoromethane was 0.23 ppb and rising at an annual rate of 0.0157 ppb(3). The avg concn of dichlorodifluoromethane in Taipei, Taiwan was 590 parts per trillion(4). Dichlorodifluoromethane was detected in Japan (1990-1991) at concns of 0.29-1.7 ppb(5).
[(1) Brodzinsky R, Singh HB; Volatile Organic Chemicals in the Atmosphere: An Assessnebt of Available Data Menlo Park CA: SRI Inter Contract 68-02-3452 (1982) (2) Su C, Goldberg ED; pp. 353-74 in Mar Pollut Transfer. Windom HL et al, eds. Lexington MA: DC Heath Co (1976) (3) Guicherit R, Schulting FL; Sci Tot Environ 43: 193-219 (1985) (4) Wang JL et al; Chemosphere 36: 2391-2400 (1998) (5) Yamasaki T et al; Wat Sci Technol 25: 33-39 (1992)]**PEER REVIEWED**


Fish/Seafood Concentrations:

None of the 47 stations in EPA's STORET database reported any dichlorodifluoromethane in biota(1).
[(1) Staples CA et al; Environ Toxicol Chem 4: 131-42 (1985)]**PEER REVIEWED**


Milk Concentrations:

In a pilot study of pollutants in the milk of women living in 4 urban-industrial areas in the US, dichlorodifluoromethane was found in 2 of 8 samples(1).
[(1) Pellizzari ED et al; Bull Environ Contam Toxicol 28: 322-8 (1982)]**PEER REVIEWED**


Environmental Standards & Regulations:

FIFRA Requirements:

Residues of dichlorodifluoromethane are exempted from the requirement of a tolerance when used as a propellant in accordance with good agricultural practices as inert (or occasionally active) ingredients in pesticide formulations applied to growing crops or to raw agricultural commodities after harvest.
[40 CFR 180.1001(c) (7/1/2000)]**PEER REVIEWED**

Dichlorodifluoromethane is exempted from the requirement of a tolerance when used as a propellant in accordance with good agricultural practice as inert (or occasionally active) ingredients in pesticide formulations applied to animals.
[40 CFR 180.1001(e) (7/1/2000)]**PEER REVIEWED**

As the federal pesticide law FIFRA directs, EPA is conducting a comprehensive review of older pesticides to consider their health and environmental effects and make decisions about their future use. Under this pesticide reregistration program, EPA examines health and safety data for pesticide active ingredients initially registered before November 1, 1984, and determines whether they are eligible for reregistration. In addition, all pesticides must meet the new safety standard of the Food Quality Protection Act of 1996. Pesticides for which EPA had not issued Registration Standards prior to the effective date of FIFRA, as amended in 1988, were divided into three lists based upon their potential for human exposure and other factors, with List B containing pesticides of greater concern and List D pesticides of less concern. Dichloromonofluoromethane is found on List D. Case No: 4042; Pesticide type: insecticide; Case Status: No products containing the pesticide are actively registered ... The case /is characterized/ as "cancelled." Under FIFRA, pesticide producers may voluntarily cancel their registered products. EPA also may cancel pesticide registrations if registrants fail to pay required fees or make/meet certain reregistration commitments, or if EPA reaches findings of unreasonable adverse effects.; Active ingredient (AI): Dichloromonofluoromethane; AI Status: The active ingredient is no longer contained in any registered pesticide products ... "cancelled."
[USEPA/OPP; Status of Pesticides in Registration, Reregistration and Special Review p.312 (Spring, 1998) EPA 738-R-98-002]**PEER REVIEWED**


Acceptable Daily Intakes:

EPA RfD= 0.2 mg/kg
[USEPA/OPP; Health Effects Div RfD/ADI Tracking Report p.20 (8/26/91)]**PEER REVIEWED**


CERCLA Reportable Quantities:

Persons in charge of vessels or facilities are required to notify the National Response Center (NRC) immediately, when there is a release of this designated hazardous substance, in an amount equal to or greater than its reportable quantity of 5000 lb or 2270 kg. The toll free number of the NRC is (800) 424-8802; In the Washington D.C. metropolitan area (202) 426-2675. The rule for determining when notification is required is stated in 40 CFR 302.4 (section IV. D.3.b).
[40 CFR 302.4 (7/1/2000)]**PEER REVIEWED**


RCRA Requirements:

U075; As stipulated in 40 CFR 261.33, when dichlorodifluoromethane, as a commercial chemical product or manufacturing chemical intermediate or an off-specification commercial chemical product or a manufacturing chemical intermediate, becomes a waste, it must be managed according to Federal and/or State hazardous waste regulations. Also defined as a hazardous waste is any residue, contaminated soil, water, or other debris resulting from the cleanup of a spill, into water or on dry land, of this waste. Generators of small quantities of this waste may qualify for partial exclusion from hazardous waste regulations (40 CFR 261.5).
[40 CFR 261.33 (7/1/2000)]**PEER REVIEWED**


Atmospheric Standards:

This action promulgates standards of performance for equipment leaks of Volatile Organic Compounds (VOC) in the Synthetic Organic Chemical Manufacturing Industry (SOCMI). The intended effect of these standards is to require all newly constructed, modified, and reconstructed SOCMI process units to use the best demonstrated system of continuous emission reduction for equipment leaks of VOC, considering costs, non air quality health and environmental impact and energy requirements. Dichlorodifluoromethane is produced, as an intermediate or a final product, by process units covered under this subpart.
[40 CFR 60.489 (7/1/2000)]**PEER REVIEWED**


Federal Drinking Water Guidelines:

EPA 1000 ug/l
[USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93)] **QC REVIEWED**


State Drinking Water Guidelines:

(AZ) ARIZONA 1400 ug/l
[USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93)] **QC REVIEWED**

(FL) FLORIDA 1,400 ug/l
[USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93)] **QC REVIEWED**

(MA) MASSACHUSETTS 1400 ug/l
[USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93)] **QC REVIEWED**

(ME) MAINE 1050 ug/l
[USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93)] **QC REVIEWED**

(MN) MINNESOTA 1000 ug/l
[USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93)] **QC REVIEWED**

(NC) NORTH CAROLINA 0.19 ug/l
[USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93)] **QC REVIEWED**

(WA) WASHINGTON 530 ug/l
[USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93)] **QC REVIEWED**


FDA Requirements:

... Essential uses of chlorofluorocarbons: (1) Metered-dose steroid human drugs for nasal inhalation; (2) Metered-dose steroid human drugs for oral inhalation; (3) Metered-dose adrenergic bronchodilator human drugs for oral inhalation; (4) Contraceptive vaginal foams for human use, and (5) Metered-dose ergotamine tartrate drug products administered by oral inhalation for use in humans; (6) Intrarectal hydrocortisone acetate for human use; (7) Polymyxin B sulfate-bacitracin zinc-neomycin sulfate soluble antibiotic powder without excipients, for topical use on humans; (8) Anesthetic drugs for topical use on accessible mucous membranes of humans where a cannula is used for application; (9) Metered-dose nitroglycerin human drugs administered to the oral cavity; (10) Metered-dose cromolyn sodium human drugs administered by oral inhalation; (11) Metered-dose ipratropium bromide for oral inhalation; (12) Metered-dose atropine sulfate aerosol human drugs administered by oral inhalation. /Chlorofluorocarbons/
[21 CFR 2.125 (4/1/91)]**PEER REVIEWED**


Allowable Tolerances:

Residues of dichlorodifluoromethane are exempted from the requirement of a tolerance when used as a propellant in accordance with good agricultural practices as inert (or occasionally active) ingredients in pesticide formulations applied to growing crops or to raw agricultural commodities after harvest.
[40 CFR 180.1001(c) (7/1/2000)]**PEER REVIEWED**

Dichlorodifluoromethane is exempted from the requirement of a tolerance when used as a propellant in accordance with good agricultural practice as inert (or occasionally active) ingredients in pesticide formulations applied to animals.
[40 CFR 180.1001(e) (7/1/2000)]**PEER REVIEWED**


Chemical/Physical Properties:

Molecular Formula:

C-Cl2-F2
[Budavari, S. (ed.). The Merck Index - Encyclopedia of Chemicals, Drugs and Biologicals. Rahway, NJ: Merck and Co., Inc., 1989. 483]**PEER REVIEWED**


Molecular Weight:

120.91
[Lide, D.R. (ed.). CRC Handbook of Chemistry and Physics. 79th ed. Boca Raton, FL: CRC Press Inc., 1998-1999.,p. 3-206]**PEER REVIEWED**


Color/Form:

LIQUEFIED COMPRESSED GAS
[U.S. Coast Guard, Department of Transportation. CHRIS - Hazardous Chemical Data. Volume II. Washington, D.C.: U.S. Government Printing Office, 1984-5.]**PEER REVIEWED**

Colorless gas ... [Note: Shipped as a liquified compressed gas].
[NIOSH. NIOSH Pocket Guide to Chemical Hazards. DHHS (NIOSH) Publication No. 97-140. Washington, D.C. U.S. Government Printing Office, 1997. 96]**PEER REVIEWED**


Odor:

Practically odorless; faint, ether-like odor in high concn
[Budavari, S. (ed.). The Merck Index - An Encyclopedia of Chemicals, Drugs, and Biologicals. Whitehouse Station, NJ: Merck and Co., Inc., 1996. 518]**PEER REVIEWED**


Boiling Point:

-29.8 deg C @ 760 mm Hg
[Lide, D.R. (ed.). CRC Handbook of Chemistry and Physics. 79th ed. Boca Raton, FL: CRC Press Inc., 1998-1999.,p. 3-206]**PEER REVIEWED**


Melting Point:

-158 deg C
[Lide, D.R. (ed.). CRC Handbook of Chemistry and Physics. 79th ed. Boca Raton, FL: CRC Press Inc., 1998-1999.,p. 3-206]**PEER REVIEWED**


Corrosivity:

Noncorrosive
[Budavari, S. (ed.). The Merck Index - An Encyclopedia of Chemicals, Drugs, and Biologicals. Whitehouse Station, NJ: Merck and Co., Inc., 1996. 518]**PEER REVIEWED**


Critical Temperature & Pressure:

Critical temp: 233.2 deg F= 111.8 deg C= 385.0 deg K; Critical pressure: 598 psia= 40.7 atm= 4.12 mn/sq m
[U.S. Coast Guard, Department of Transportation. CHRIS - Hazardous Chemical Data. Volume II. Washington, D.C.: U.S. Government Printing Office, 1984-5.]**PEER REVIEWED**


Density/Specific Gravity:

1.486 @ -29.8 deg C
[Budavari, S. (ed.). The Merck Index - An Encyclopedia of Chemicals, Drugs, and Biologicals. Whitehouse Station, NJ: Merck and Co., Inc., 1996. 518]**PEER REVIEWED**


Heat of Combustion:

Nonflammable
[Lewis, R.J., Sr (Ed.). Hawley's Condensed Chemical Dictionary. 13th ed. New York, NY: John Wiley & Sons, Inc. 1997. 364]**PEER REVIEWED**


Heat of Vaporization:

140 Btu/lb= 77.9 cal/g= 3.26X10+5 J/kg (Latent)
[U.S. Coast Guard, Department of Transportation. CHRIS - Hazardous Chemical Data. Volume II. Washington, D.C.: U.S. Government Printing Office, 1984-5.]**PEER REVIEWED**


Octanol/Water Partition Coefficient:

log Kow= 2.16
[Hansch, C., Leo, A., D. Hoekman. Exploring QSAR - Hydrophobic, Electronic, and Steric Constants. Washington, DC: American Chemical Society., 1995. 3]**PEER REVIEWED**


Solubilities:

0.28 g/l water @ 25 deg C @ 1 atm
[Flick, E.W. Industrial Solvents Handbook. 3rd ed. Park Ridge, NJ: Noyes Publications, 1985. 86]**PEER REVIEWED**

1.9 g/l water @ 25 deg C @ 94.4 psia (the propellant vapor pressure)
[Flick, E.W. Industrial Solvents Handbook. 3rd ed. Park Ridge, NJ: Noyes Publications, 1985. 86]**PEER REVIEWED**

13.1 wt% amyl chloride @ 21.1 deg C @ 1 atm
[Flick, E.W. Industrial Solvents Handbook. 3rd ed. Park Ridge, NJ: Noyes Publications, 1985. 86]**PEER REVIEWED**

9.0 wt% benzene @ 21.l deg C @ 1 atm
[Flick, E.W. Industrial Solvents Handbook. 3rd ed. Park Ridge, NJ: Noyes Publications, 1985. 86]**PEER REVIEWED**

5.0 wt% bromobenzene @ 21.1 deg C @ 1 atm
[Flick, E.W. Industrial Solvents Handbook. 3rd ed. Park Ridge, NJ: Noyes Publications, 1985. 86]**PEER REVIEWED**

1.2 wt% bromoform @ 21.1 deg C @ 1 atm
[Flick, E.W. Industrial Solvents Handbook. 3rd ed. Park Ridge, NJ: Noyes Publications, 1985. 86]**PEER REVIEWED**

8.5 wt% n-butyl alcohol @ 21.1 deg C @ l atm
[Flick, E.W. Industrial Solvents Handbook. 3rd ed. Park Ridge, NJ: Noyes Publications, 1985. 86]**PEER REVIEWED**

13.2 wt% butyl butyrate @ 21.1 deg C @ 1 atm
[Flick, E.W. Industrial Solvents Handbook. 3rd ed. Park Ridge, NJ: Noyes Publications, 1985. 86]**PEER REVIEWED**

5.2 wt% carbon tetrachloride @ 21.1 deg C @ 1 atm
[Flick, E.W. Industrial Solvents Handbook. 3rd ed. Park Ridge, NJ: Noyes Publications, 1985. 86]**PEER REVIEWED**