Abstract

Objective: To observe the change of stanniocalcin 1 (STC1) and calcium content in brain of coal-burning-borne fluorosis rats, and to explore the role of STC1 in brain injury of coal-burning-borne fluorosis.

Methods: Twenty four male SD rats were randomly divided into control, low, medium, and high fluoride groups according to body mass.Control group was fed conventional rat chow (fluorinated 1.3 mg / kg), and low, medium and high fluoride groups fed with fluorinated feed (20.0,40.0, 60.0 mg / kg). All rats were given distilled water and feed ad libitum. One hundred and eighty days after modeling, STC1 protein and gene expression in the brain tissue of rats were detected using immunohistochemistry and RT-PCR and calcium content of brain tissue was detected.

Results: The cell positive rates of STC1 in low, medium, high fluoride groups [(48.10 + 2.11)%, (54.90 ± 1.73)%, (79.30 ± 3.71)%] were significantly higher than that of the control group [(24.70 + 3.53)%, all P <0.05], the cell positive rate of high fluoride group was significantly higher than that of the low and medium fluoride groups (all P <0.05). The STC1 mRNA expression of low, medium and high fluoride groups (0.58 ± 0.09,0.85 ± 0.17,1.75 ± 0.04) were significantly higher than that in the control group (0.37 ± 0.12, all P <0.05), the STC1 mRNA expressions of high fluoride group was significantly higher than that of the low and medium fluoride groups (all P <0.05). The brain cortex calcium ion concentrations of low, medium and high fluoride groups [(138.62 + 4.19), (167.43 + 6.57), (189.45 + 3.72) nmol/L] were significantly higher than that in the control group [(101.47 + 9.46) nmol/L, all P <0.05], the brain cortex calcium ion concentrations of high fluoride group was significantly higher than that of the low and medium fluoride groups (all P <0.05 ), and the medium fluoride groups was higher than the low groups (P <0.05).

Conclusion: STC 1 may be involved in brain damage of coal-burning-borne fluorosis rats through regulating calcium balance.

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