Abstract

OBJECTIVE: To observe certain changes of oxidation, anti-oxidation and vascular function indexes of Application of New Zealand rabbit exposed by high-fluoride.

METHODS: 20 male New Zealand rabbits were randomly divided into control group, drunk deionized water and feed basic diet. High-fat group, drunk deionized water and feed basic diet plus 0.5% cholesterol and 7% egg yolk powder high fat diet. High-fluorine group, drunk high-fluoride water (ion-100 mg/L) and feed basic diet. High-fluoride and high-fat group, drunk high-fluoride water (ion-100 mg/L) and feed basic diet plus 0.5% cholesterol and 7% egg yolk powder high fat diet. The experimental periods were 6 months. Blood samples were collected to determine the fluorine concentration in plasma, in the third and sixth month before experiment. In the sixth month of the experiment, blood, heart and liver samples were gathered to make homogenate, and detect superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) contents by biochemical method. The 6-keto-prostate F1alpha (6-keto-PGF1alpha), thromboxane B2 (TXB2) and endothelin-1(ET-1) contents were detected by radioimmunoassay. Nitric oxide synthase (NOS), inducible NOS (iNOS), and endothelial NOS (eNOS) were detected by biochemical method. Leukocyte iNOS-mRNA and eNOS-mRNA contents were detected by situ hybridization.

RESULTS: In the third and sixth month of experiment, serum fluorides were elevated in rabbits who drunk high-fluorine water, activities of SOD and GSH-Px in blood, liver and heart were decreased (P < 0.01, P < 0.05), while the myocardial MDA contents increased (P < 0.05), 6-keto-PGF1alpha contents in plasma decreased (P < 0.01), TXB2 and ET-1 levels increased (P < 0.01, P < 0.05), total NOS activities in serum decreased (P < 0.05), total NOS activities in liver and iNOS activities in heart and liver increased (P < 0.05). Expression of iNOS-mRNA in leukocyte increased, expression of eNOS-mRNA decreased (P < 0.05) in rabbits who drunk high-fluorine in the sixth month. Factorial analysis of variance, serum, liver and myocardial SOD activities and serum MDA contents, plasma ET-1 contents and serum iNOS activities, liver total NOS activities showed that high-fluorine and high-fat enhanced interactive (P < 0.01 P < 0.05) .

CONCLUSION: High-fluoride could inhibit antioxidant enzymes, impair vascular endothelial function, body NO metabolism disorder. High fluoride and high-fat could have a certain synergy in this process.

• Related Studies :

  • Elevated serum fluoride levels in perimenopausal women are related to the components of metabolic syndrome.

    OBJECTIVE: Increased fluoride levels can lead to numerous complications, including skeletal effects, cardiotoxicity, endocrine dysfunction, neurotoxicity, hepatotoxicity and nephrotoxicity. The aim of this study was to analyze the relationship between serum fluoride levels and MetS or its individual components, and to assess the diagnostic usefulness of fluoride as a factor contributing to

  • Association between fluoride exposure and cardiometabolic risk in peripubertal Mexican children.

    Highlights Fluoride has been added to table salt in Mexico to reduce dental caries. Human studies of fluoride exposure and metabolic syndrome are rare. We examined the association between fluoride and cardiometabolic risk factors. Fluoride may increase the risk of cardiometabolic disorders in Mexican girls. BACKGROUND: Several animal studies have suggested

  • The pharmacology of sevoflurane in infants and children.

    BACKGROUND: Sevoflurane is a new volatile anesthetic with physical properties that should make it suitable for anesthesia (MAC of sevoflurane on oxygen alone and in 60% nitrous oxide, (MAC) of sevoflurane in oxygen alone and in 60% nitrous oxide, the hemodynamic, induction and emergence responses to sevoflurane and the metabolism

  • The LD50, excretion and serum and bone levels of F after a high single F and F + Mg dose in rats with findings on cardiac Ca and Mg.

    The LD50 for fluoride was elevated from less than 60 mg F/kg body weight to 172 mg F/kg when magnesium (as MgCl2), equivalent to 3 times that of F, was administered by gavage 30 min after the F dose. A dose of 30 mg F/kg elevated the mean steady state

  • Inorganic plasma fluoride concentrations and its renal excretion in certain physiological and pathological conditions in man

    In a study involving 2200 patients the inorganic plasma fluoride concentration (IPFC) increased with increasing age. In a fluoridated (1 ppm) community this increase was more pronounced than in a low fluoride (0.2 ppm) community. The mean renal clearance of fluoride and the daily amounts excreted also increased slightly until

• Related FAN Content :

  • Fluoride & Myocardial Damage

    Structural damage to the heart resulting from fluoride toxicity has been observed in numerous human and animal studies. The general features of this damage include cloudy swelling, vacuolization or vacuolar degeneration, hemorrhages, interstitial edema, fibrous necrosis, dissolution of nuclei, and thickening of the vessel walls in the heart muscle (Basha

  • Fluoride & Arteriosclerosis

    Healthy arteries are flexible and elastic, allowing efficient transfer of blood and nutrients from the heart to the rest of the body. Arteriosclerosis refers to a stiffening of the arteries, including loss of elasticity. This is a slow, progressive disease that may begin early in life from damage to the

  • Fluoride, Blood Pressure and Hypertension

    Individuals with blood pressure readings that exceed 140/90 are considered hypertensive. Hypertension can increase the risk of stroke, heart attack, heart failure, aortic aneurysms, and peripheral arterial disease. An association between increased fluoride in ground water and increased prevalence of hypertension has been observed, especially among adult males (Amini et

  • Fluoride & Arterial Calcification

    The major change involved with cardiovascular disease is development of atherosclerosis in critical arteries, which is partially characterized by vascular calcification. The level of coronary artery calcification is thought to be the most important indicator of future cardiovascular events. Increased arterial calcifications have frequently been reported in those with skeletal fluorosis

  • Fluoride & Electrocardiogram Abnormalities

    An electrocardiogram (ECG) is a diagnostic test that measures the electrical activity of the heart. An ECG can reveal heart rate, heart rhythym (i.e. steady or irregular), and the strength and timing of the heart’s natural electrical signals. ECGs are described in terms of “waves” (e.g. amplitude and duration). Problems