OBJECTIVE: To observe certain changes of oxidation, anti-oxidation and vascular function indexes of Application of New Zealand rabbit exposed by high-fluoride.
METHODS: 20 male New Zealand rabbits were randomly divided into control group, drunk deionized water and feed basic diet. High-fat group, drunk deionized water and feed basic diet plus 0.5% cholesterol and 7% egg yolk powder high fat diet. High-fluorine group, drunk high-fluoride water (ion-100 mg/L) and feed basic diet. High-fluoride and high-fat group, drunk high-fluoride water (ion-100 mg/L) and feed basic diet plus 0.5% cholesterol and 7% egg yolk powder high fat diet. The experimental periods were 6 months. Blood samples were collected to determine the fluorine concentration in plasma, in the third and sixth month before experiment. In the sixth month of the experiment, blood, heart and liver samples were gathered to make homogenate, and detect superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) contents by biochemical method. The 6-keto-prostate F1alpha (6-keto-PGF1alpha), thromboxane B2 (TXB2) and endothelin-1(ET-1) contents were detected by radioimmunoassay. Nitric oxide synthase (NOS), inducible NOS (iNOS), and endothelial NOS (eNOS) were detected by biochemical method. Leukocyte iNOS-mRNA and eNOS-mRNA contents were detected by situ hybridization.
RESULTS: In the third and sixth month of experiment, serum fluorides were elevated in rabbits who drunk high-fluorine water, activities of SOD and GSH-Px in blood, liver and heart were decreased (P < 0.01, P < 0.05), while the myocardial MDA contents increased (P < 0.05), 6-keto-PGF1alpha contents in plasma decreased (P < 0.01), TXB2 and ET-1 levels increased (P < 0.01, P < 0.05), total NOS activities in serum decreased (P < 0.05), total NOS activities in liver and iNOS activities in heart and liver increased (P < 0.05). Expression of iNOS-mRNA in leukocyte increased, expression of eNOS-mRNA decreased (P < 0.05) in rabbits who drunk high-fluorine in the sixth month. Factorial analysis of variance, serum, liver and myocardial SOD activities and serum MDA contents, plasma ET-1 contents and serum iNOS activities, liver total NOS activities showed that high-fluorine and high-fat enhanced interactive (P < 0.01 P < 0.05) .
CONCLUSION: High-fluoride could inhibit antioxidant enzymes, impair vascular endothelial function, body NO metabolism disorder. High fluoride and high-fat could have a certain synergy in this process.