In the present study, forty villages in Mehsana district and thirteen villages in Banaskantha district were surveyed and the effects of waterborne fluoride were investigated. Parallel studies were also conducted in Ahmedabad city (non-fluoride endemic area) population which was considered as control to compare the changes occurring due to consumption of high water borne fluoride in endemic population. For each individual, a detailed proforma sheet for health and water quality survey was filled up from each village, which included name, age, sex and address of the individual, food habbits, mottling of teeth, dental caries in premolars and canines as well as skeletal alterations etc…

RESULTS AND DISCUSSION
In the present study, analysis of water samples collected from 15 different localities in Ahmedabad city showed a low concentration of 0.5 ppm fluoride to a maximum of 0.72 ppm with a mean of 0.64±0.013 ppm in water, while the survey conducted in 53 villages revealed wide variation in the levels of fluoride from a minimum of 1 ppm to a maximum of 6.53 ppm having a mean of 2.81±0.179ppm.

… Na+ and K+ levels in serum showed significant increase due to consumption of higher levels of fluoride in drinking water in case of fluorotic population as compared to the control population of Ahmedabad city (Table 2).

… The results in the present study revealed a significant decline in the levels of blood glucose in fluoride afflicted human population of North Gujarat as compared to control (Table 2).

… Similarly, the serum epinephrine and nor-epinephrine levels were increased significantly. This might be due to the stress on adrenal gland or else enhanced release of these hormones due to accumulation of fluoride in body (Patel et al., 1994).

… The levels of serum triiodothyronine (T3), thyroxin (T4), Follicle Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and testosterone are summarized in Table 3. In this study, people residing in fluoride endemic regions did not exhibit any significant alteration in T3 and TSH levels, while T4 levels were comparatively enhanced as compared to the population in Ahmedabad city. An increase in the levels of serum thyroxin might be due to its enhanced synthesis or decline in its utilization…