An understanding of the rate at which fluorine (F) accumulates in soil from phosphorus fertiliser and how much is lost via leaching are important because of the challenge elevated F may pose to soil, plant and animal health. This study measured F accumulation in a soil under pasture (Inceptisol) that received single superphosphate (SSP) fertiliser over 63?years in a long-term fertiliser trial. It also assessed the downward movement of F in soil and effect of irrigation in a long-term irrigation trial. Results showed total F concentrations in topsoil (0–7.5?cm) that received 188?kg SSP?ha^?1 and 376?kg SSP?ha^?1 fertiliser increased from 251?mg?kg^?1 to 349 and 430?mg?kg^?1, respectively. The rates of F accumulation were estimated at 1.1 and 2.1?kg?ha^?1?yr^?1 respectively, low compared to previous studies. The F concentration in the 376?kg SSP ha^?1 treatment decreased with depth, but was significantly higher than the control treatment to 50?cm, suggesting movement of F down the soil. Fluorine concentrations also decreased with depth to 50?cm in soils that received either no irrigation or up to 770?mm?yr^?1 from irrigation. However there was no significant difference in the amount of F with depth between irrigation treatments. We postulate that F was moving predominately in drainage water in the period immediately after P fertiliser application in mid-winter and wasn’t enhanced by irrigation. This study should be repeated for other soil types to help our understanding of the potential for ongoing F accumulation in soil and implications this may have on future land use.