Abstract

The accumulation of fluoride in tea leaves from various cultivars exhibits significant differences. However, the molecular basis and mechanism remains largely unknown. Here, we reported that two genes of CsFEXs (Fluoride export genes in C. sinensis), CsFEX1 and CsFEX2 transport fluoride out of cells, alleviate the cellular fluoride toxin and rescue the yeast mutant (FEX1?FEX2?) and Arabidopsis mutant (fex), their efflux activities are coupled with proton gradients. Further analysis found that CsFEX1 and CsFEX2 localize to the plasma membrane both in yeast and Arabidopsis cells. CsFEX2 is more effective to reduce the fluoride toxicity in yeast and Arabidopsis compared with that of CsFEX1 even at low pH. CsFEX2 induced by fluoride treatment is around 10-fold higher in low-fluoride cultivar (‘Yunkang 10’) than that in high-fluoride cultivar (‘Pingyang Tezaocha’), suggesting that CsFEX2 possibly functions as a critical role in reducing the fluoride accumulation in tea leaves.

*Original abstract online at https://pubs.acs.org/doi/10.1021/acs.jafc.0c03444

• Related Studies :

  • Amelioration by black tea of sodium fluoride-induced changes in protein content of cerebral hemisphere, cerebellum and medulla oblongata in brain region of mice.

    Oral administration of sodium fluoride (NaF, 6 and 12 mg/kg body weight/day) to Swiss strain male albino mice for 30 days caused significant dose-dependant reduction in the content of acidic, basic, neutral, and total protein in cerebral hemisphere, cerebellum and medulla oblongata region of brain. After 30 days of NaF

  • Brick tea fluoride as a main source of adult fluorosis

    An epidemiological survey was conducted in Naqu County, Tibet in September 2001 to investigate the manifestations of fluorosis in adults caused by the habitual consumption of brick tea. Profiles were obtained for the total daily fluoride intake, environmental fluoride levels and average urinary fluoride concentration, and a physical examination and a

  • Fluoride intake from the consumption of refreshment drinks and natural juices.

    Highlights Soft-drinks and juices (65) were analyzed by potentiometry. Tea-based beverages had the highest fluoride content. Fluoride intake does not lead to a risk in adult health. Consumption of these beverages by children should be moderated. Fluoride plays an important role in the prevention of dental decay and in the reduction

  • Analysis of naturally occurring fluoride in commercial teas and estimation of its daily intake through tea consumption

    The aim of this study was to determine the levels of fluoride in commercial teas and to estimate the contribution of tea consumption to the fluoride recommended daily allowance. A total of 558 tea products in 6 categories, green tea, black tea, oolong tea, pu'er tea, white tea, and reprocessed

  • Comparison of total ionic strength adjustment buffers III and IV in the measurement of fluoride concentration of teas.

    BACKGROUND: Tea is the second most consumed drink in the UK and a primary source of hydration; it is an important source of dietary fluoride (F) for consumers and also abundant in aluminium (Al). Varying ranges of F concentrations in teas have been reported worldwide which may be, in part,

• Related FAN Content :

  • Fluoride Content of Tea

    Tea, particularly tea drinks made with lower quality older leaves, contain high levels of fluoride. Because of these high levels, research has found that individuals who drink large amounts of tea can develop skeletal fluorosis -- a painful bone disease caused by excessive fluoride intake. Since skeletal fluorosis is often misdiagnosed by

  • Tea Intake Is a Risk Factor for Skeletal Fluorosis

    A number of recent studies have found that heavy tea drinkers can develop skeletal fluorosis - a bone disease caused by excessive intake of fluoride.

  • Fluoride content in tea and its relationship with tea quality.

    J Agric Food Chem. 2004 Jul 14;52(14):4472-6. Fluoride content in tea and its relationship with tea quality. Lu Y, Guo WF, Yang XQ. Department of Tea Science, Zhejiang University, 268 Kaixuan Road, Hangzhou 310027, People's Republic of China. Abstract: The tea plant is known as a fluorine accumulator. Fluoride (F) content in fresh leaves collected

  • The Lancet: Fluoride Studies in a Patient with Arthritis

    It is possible that fluoride intake from tea may be sufficient to cause fluorosis, and I report here a case which gives some evidence for this.

  • Estimated "Threshold" Doses for Skeletal Fluorosis

    For over 40 years health authorities stated that in order to develop crippling skeletal fluorosis, one would need to ingest between 20 and 80 mg of fluoride per day for at least 10 or 20 years. This belief, however, which played an instrumental role in shaping current fluoride policies, is now acknowledged by the National Academy of Sciences (NAS) and other US health authorities to be incorrect.