Abstract
Reaction with methyl thiazolyl tetrazolium (MTT) was used to investigate in vitro stimulatory (hormesis) effects of sodium fluoride (NaF) on kidney cells collected from three-day-old Sprague-Dawley male rats. The cell cultures were exposed to incremental concentrations of NaF ranging from of 0 (control) to 160 ?mol/L and from 500 to 16,000 ?mol/L. The mean optical density (OD) ± SD decreased from the control value of 0.591±0.119 to a minimum of 0.468±0.065 at 20 ?mol NaF/L before returning to the control level near 160 ?mol/L. At 500 ?mol/L the OD was 0.545±0.066, after which it decreased monotonically to 0.387±0.046 at 4000 ?mol/L, with cell death being complete at 16,000 ?mol/L. These results indicate that 20 ?mol/L is the lowest concentration at which a stimulatory (hormesis) effect of NaF is observed in kidney cell cultures of very young rats. In addition, results of flow cytometry and RNA detection confirmed these MTT findings.
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Effects of chronic fluoride intake on the antioxidant systems of the liver and kidney in rats
Excessive fluoride intake over a long period of time can lead to fluorosis, which may cause dental and skeletal manifestations. Metabolic, functional and structural damage caused by chronic fluorosis have been reported in many tissues, but the exact mechanisms modulated by fluoride remain unclear. The aim of this study was
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Reproductive and developmental toxicity of degradation products of refrigerants in experimental animals
The present paper summarizes the results of animal studies on the reproductive and developmental toxicity of the degradation products of refrigerants, including trifluoroacetic acid (TFA), carbon dioxide (CO(2)), carbon monoxide (CO), carbonyl fluoride (CF), hydrogen fluoride (HF) and formic acid (FA). Excessive CO(2) in the atmosphere is testicular and reproductive
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Influence of sodium fluoride and caffeine on the kidney function and free-radical processes in that organ in adult rats
An experiment was carried out on Sprague-Dawley rats (adult males) that for 50 days were administered, in the drinking water, NaF and NaF with caffeine (doses, respectively: 4.9 mg of NaF/kg body mass/24 h and 3 mg of caffeine/kg body mass/24 h). Disturbances were noted in the functioning of kidneys,
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Strong acute toxicity, severe hepatic damage, renal injury and abnormal serum electrolytes after intravenous administration of cadmium fluoride in rats
Cadmium fluoride (CdF) is commonly used as an insulator for ulta high speed mass telecommunications equipment, and there is a considerable risk that industrial workers will inhale CdF particles. Despite the possibility that acute exposure can cause harmful systemic effects, there are no studies to date that address the health
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The structural and biochemical changes of kidney tissue on fluorosis in rabbit
In this study, the effects of sodium fluoride given subchronically at the doses of 1, 10 and 40 mg/L for 70 days were investigated. For this purpose, 21 male New Zealand rabbits were used. At the end of the 70th day, the level of blood urea nitrogen (BUN), creatine (CRE)
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Fluoride as a Cause of Kidney Disease in Humans
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