Abstract
Reaction with methyl thiazolyl tetrazolium (MTT) was used to investigate in vitro stimulatory (hormesis) effects of sodium fluoride (NaF) on kidney cells collected from three-day-old Sprague-Dawley male rats. The cell cultures were exposed to incremental concentrations of NaF ranging from of 0 (control) to 160 ?mol/L and from 500 to 16,000 ?mol/L. The mean optical density (OD) ± SD decreased from the control value of 0.591±0.119 to a minimum of 0.468±0.065 at 20 ?mol NaF/L before returning to the control level near 160 ?mol/L. At 500 ?mol/L the OD was 0.545±0.066, after which it decreased monotonically to 0.387±0.046 at 4000 ?mol/L, with cell death being complete at 16,000 ?mol/L. These results indicate that 20 ?mol/L is the lowest concentration at which a stimulatory (hormesis) effect of NaF is observed in kidney cell cultures of very young rats. In addition, results of flow cytometry and RNA detection confirmed these MTT findings.
-
-
Changes in fluoride levels in the liver, kidney, and brain and in neurotransmitters of mice after subacute administration of fluoride
The effects of fluoride after subacute oral administration of NaF at levels of 0, 1, 5, 25, and 125 ppm F– were evaluated in adult male BALB/c mice. Fluoride levels in the murine liver, kidney, and cerebrum after one month were determined using a highly sensitive flow-injection apparatus with a
-
Significance of Mg-hardness and fluoride in drinking water on chronic kidney disease of unknown etiology in Monaragala, Sri Lanka.
Highlights Geochemistry of groundwater in a CKDu hotspot was investigated as a case-control study. Comparatively higher Mg and fluoride levels were found in affected regions. Excess fluoride and hardness in drinking water could enhance the tubular injury. Nephrotoxic trace elements such as As, Pb, and Cd, etc. were below
-
A Tragedy of Relying on Misinformation: Import Ban on Synthetic Fertilizers.
... Agrochemicals are generally considered as the causal factors for many of the non-communicable diseases (NCDs), especially the chronic kidney disease of uncertain etiology (CKDu). Such unproven ideology has been forced into minds of people who are suffering from the disease. Some even dubbed CKDu as ‘Agricultural kidney disease’. This
-
Effects of selenium intervention on chronic fluorosis-induced renal cell apoptosis in rats
This study aims to explore the effect of selenium in fluoride-induced renal cell apoptosis in rats and determine the optimal level of selenium in drinking water to prevent fluorosis. Experimental animals were divided into a control group, a sodium fluoride-treated group (NaF, 50 mg/L), three sodium selenite-treated groups (Na2SeO3, 0.375, 0.75,
-
Effect of fluoride on major organs with the different time of exposure in rats.
BACKGROUND: High fluoride levels in drinking water in relation to the prevalence of chronic kidney disease of unknown etiology (CKDu) in Sri Lanka were investigated using rats as an experimental model. METHOD: The effects of fluoride after oral administration of Sodium fluoride (NaF) at levels of 0, 0.5, 5 and 20 ppm F- were evaluated
Related Studies :
-
-
-
Fluoride & Kidney Stones
It has long been suspected that fluoride may contribute to the formation of kidney stones. This suspicion has recently gained support from a study of an American man with skeletal fluorosis. According to the authors: "A new, important, medical problem (that seemed temporally related to cessation of fluoride exposure and subsequent negative calcium
-
Fluoride as a Cause of Kidney Disease in Humans
Because the kidney is exposed to higher concentrations of fluoride than all other soft tissues (with the exception of the pineal gland), there is concern that excess fluoride exposure may contribute to kidney disease - thus initiating a "vicious cycle" where the damaged kidneys increase the accumulation of fluoride, causing
-
Kidney: A potential target for fluoride toxicity
The kidneys are the organ responsible for clearing fluoride from the body. In the process of doing so, the kidneys are exposed to concentrations of fluoride that exceed, by a factor of 50, the concentration of fluoride in human blood. As such, the kidney have long been considered a potential
-
Fluoride as a Cause of Kidney Disease in Animals
Because the kidney is exposed to higher concentrations of fluoride than all other soft tissues (with the exception of the pineal gland), there is concern that excess fluoride exposure may contribute to kidney disease - thus initiating a "vicious cycle" where the damaged kidneys increase the accumulation of fluoride, causing in
-
Fluoride Gels & Kidney Function
Scientists have found that the application of "Fluoride Gels" at the dental office causes very high spikes in the blood fluoride level. The high spikes in blood fluoride levels are a result of three factors: the high concentration of fluoride in the gel (= 12.3 mg of fluoride in each
Related FAN Content :
-