Abstract

Reaction with methyl thiazolyl tetrazolium (MTT) was used to investigate in vitro stimulatory (hormesis) effects of sodium fluoride (NaF) on kidney cells collected from three-day-old Sprague-Dawley male rats. The cell cultures were exposed to incremental concentrations of NaF ranging from of 0 (control) to 160 ?mol/L and from 500 to 16,000 ?mol/L. The mean optical density (OD) ± SD decreased from the control value of 0.591±0.119 to a minimum of 0.468±0.065 at 20 ?mol NaF/L before returning to the control level near 160 ?mol/L. At 500 ?mol/L the OD was 0.545±0.066, after which it decreased monotonically to 0.387±0.046 at 4000 ?mol/L, with cell death being complete at 16,000 ?mol/L. These results indicate that 20 ?mol/L is the lowest concentration at which a stimulatory (hormesis) effect of NaF is observed in kidney cell cultures of very young rats. In addition, results of flow cytometry and RNA detection confirmed these MTT findings.

• Related Studies :

  • Renal tubular site of action of fluoride in Fischer 344 rats

    Methoxyflurane is capable of producing high-output renal failure in some patients and animal models, probably through metabolic liberation of free fluoride. The tubular site of action of fluoride was examined in Fischer 344 rats using clearance techniques. Free water reabsorption (TCH2O) and free water excretion (CH2O) were measured during mannitol

  • Renal proteome in mice with different susceptibilities to fluorosis

    A/J and 129P3/J mouse strains have different susceptibilities to dental fluorosis due to their genetic backgrounds. They also differ with respect to several features of fluoride (F) metabolism and metabolic handling of water. This study was done to determine whether differences in F metabolism could be explained by diversities in

  • Histopathological changes of renal tissue following sodium fluoride administration in two consecutive generations of mice. Correlation with the urinary elimination of fluoride

    The present study was designed to investigate the toxic effects (evaluated as histopathological changes) of sodium fluoride on the kidney in two consecutive generations of NMRI mice. An attempt to correlate the toxicity with the urinary elimination of fluoride has been made, as urinary fluoride excretion has been widely used

  • Effect of sodium fluoride on concentrating and diluting ability in the rat

    Mechanisms for the concentrating defect produced by fluoride were examined in the rat. Free-water clearance at all levels of delivery was normal after 5 days of chronic fluoride administration in the hereditary hypothalamic diabetes insipidus rat. In the Sprague-Dawley rats, during moderate fluoride administration (120 micronmol/kg per day), urine osmolality

  • Effects of selenium and zinc on renal oxidative stress and apoptosis induced by fluoride in rats.

    OBJECTIVE: To study the effects of selenium and zinc on oxidative stress, apoptosis, and cell cycle changes in rat renal cells induced by fluoride. METHODS: Wistar rats were given distilled water containing sodium fluoride (50 mg/L NaF) and were gavaged with different doses of selenium-zinc preparation for six months. Four groups

• Related FAN Content :

  • Fluoridation of drinking water and chronic kidney disease: Absence of evidence is not evidence of absence

    A fairly substantial body of research indicates that patients with chronic renal insufficiency are at an increased risk of chronic fluoride toxicity. Patients with reduced glomerular filtration rates have a decreased ability to excrete fluoride in the urine. These patients may develop skeletal fluorosis even at 1 ppm fluoride in the drinking water.

  • Kidney: A potential target for fluoride toxicity

    The kidneys are the organ responsible for clearing fluoride from the body. In the process of doing so, the kidneys are exposed to concentrations of fluoride that exceed, by a factor of 50, the concentration of fluoride in human blood. As such, the kidney have long been considered a potential

  • Fluoride & Kidney Stones

    It has long been suspected that fluoride may contribute to the formation of kidney stones. This suspicion has recently gained support from a study of an American man with skeletal fluorosis. According to the authors: "A new, important, medical problem (that seemed temporally related to cessation of fluoride exposure and subsequent negative calcium

  • Fluoride as a Cause of Kidney Disease in Animals

    Because the kidney is exposed to higher concentrations of fluoride than all other soft tissues (with the exception of the pineal gland), there is concern that excess fluoride exposure may contribute to kidney disease - thus initiating a "vicious cycle" where the damaged kidneys increase the accumulation of fluoride, causing in

  • Fluoride Gels & Kidney Function

    Scientists have found that the application of "Fluoride Gels" at the dental office causes very high spikes in the blood fluoride level. The high spikes in blood fluoride levels are a result of three factors: the high concentration of fluoride in the gel (= 12.3 mg of fluoride in each