Abstract
The present study was designed to examine an ability of inorganic fluoride (F) to induce oxidative stress and energy depletion in the rat erythrocytes in vitro. Accumulation of ROS and alterations in glutathione (GSH) and ATP contents were estimated in the cells incubated with 0.1-10mM NaF for 1, 5 and 24h. Exposure of the rat erythrocytes to NaF was accompanied by progressive accumulation of peroxides, while superoxide (O(2)(-)) production was insignificant. Intracellular GSH content was reduced following 5-h incubation, but considerably elevated after 24h, although GSH/GSSG ratio decreased in both cases. ATP concentration in the NaF-treated cell exhibited a dose- and time-dependent decline, diminishing to extremely low levels within 24h. Thus, exposure of the rat erythrocytes to NaF leads to impairment of the cellular antioxidant system and severe energy depletion, the latter probably being the primary toxic effect.
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Toxic effects of fluoride and chlorpyrifos on antioxidant parameters in rats: protective effects of vitamins C and E
In continuing our studies on the effects of fluoride (F) on the toxicity of pesticides, we investigated the interaction of 1 ppm and 10 ppm F in the drinking water of rats orally administered 1 and 10 mg chlorpyriphos/kg bw/day, alone and in combination for 28 days. Changes in antioxidant
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Cytoprotective effects of curcumin on sodium fluoride-induced intoxication in rat erythrocytes
Curcumin is well known for its potent antioxidant activity. The result of numerous studies showed that antioxidants can protect against fluoride-induced toxicity. In the present study, protective effects of curcumin against sodium fluoride-induced toxicity in rat erythrocytes were evaluated. Curcumin (10 and 20 mg/kg) and vitamin C (10 mg/kg) were
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Effect of sodium fluoride ingestion on malondialdehyde concentration and the activity of antioxidant enzymes in rat erythrocytes
Fluoride intoxication has been shown to produce diverse deleterious metabolic alterations within the cell. To determine the effects of sodium fluoride (NaF) treatment on malondialdehyde (MDA) levels and on the activity of antioxidant enzymes in rat erythrocytes, Male Wistar rats were treated with 50 ppm of NaF or were untreated
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Lipid peroxidation in fluorosis and the protective role of dietary factors
The influence of chronic Fl intoxication on lipid peroxidation and the state of the antioxidant system was studied in rats on different diets. Chronic Fl intoxication inhibited antioxidant activity and caused an increase in the rate of peroxidation and the level of lipoperoxides in liver, brain and serum. Diets with
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Antioxidant defense systems in red blood cell lysates of men with dental fluorosis living in Tamil Nadu, India
The status of lipid peroxidation (LPO) and antioxidants was studied in red cell blood lysates of male subjects, aged 41-50, living in an endemic fluorosis area, Vellore district, Tamil Nadu, India. The men were divided into four groups: 1) normal healthy individuals (n=10); 2) individuals with mild dental fluorosis (n=13);
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Fluoride content in tea and its relationship with tea quality.
J Agric Food Chem. 2004 Jul 14;52(14):4472-6. Fluoride content in tea and its relationship with tea quality. Lu Y, Guo WF, Yang XQ. Department of Tea Science, Zhejiang University, 268 Kaixuan Road, Hangzhou 310027, People's Republic of China. Abstract: The tea plant is known as a fluorine accumulator. Fluoride (F) content in fresh leaves collected
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Nutrient Deficiencies Enhance Fluoride Toxicity
It has been known since the 1930s that poor nutrition enhances the toxicity of fluoride. As discussed below, nutrient deficiencies have been specifically linked to increased susceptibility to fluoride-induced tooth damage (dental fluorosis), bone damage (osteomalacia), neurotoxicity (reduced intelligence), and mutagenicity. The nutrients of primary importance appear to be calcium,
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Fluoride & Oxidative Stress
A vast body of research demonstrates that fluoride exposure increases oxidative stress. Based on this research, it is believed that fluoride-induced oxidative stress is a key mechanism underlying the various toxic effects associated with fluoride exposure. It is also well established that fluoride's toxic effects can be ameliorated by exposure
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