Abstract

The percentage of spermatozoa in ram semen with intact acrosomes and the level of spermatozoa motility decreased significantly after dilution and after 5 hr incubation at 38ºC. Both indices decreased significantly in the presence of NaF at concentrations ranging from 20 µmol/L to 0.1 mol/L. The activities of androgen-dependent enzymes—acid phosphatase (ACP), lactate dehydrogenase (LDH), and gamma-glutamyl transferase (y-GT-10S)—decreased significantly when the ejaculate was treated with NaF at concentrations of 20, 100, 200 µmol/L (0.38; 1.9; 3.8 ppm F-), but they returned to the initial value of the control at 0.1 mol/L (1900 ppm F-). The activity of aspartate transaminase (AspAT) displayed a large increase with the increasing lower F-concentrations and then showed a substantial decrease in activity at the high F- concentration. These changes undoubtedly affect the physiological functions of the sperm.

• Related Studies :

  • Induction of oxidative stress on reproductive and metabolic organs in sodium fluoride-treated male albino rats: protective effect of testosterone and vitamin E coadministration

    The present study was undertaken to search out the effect of sodium fluoride, a water pollutant noted throughout the world, including India, on oxidative stress induction in reproductive tissues, sperm pellet, and metabolic tissues like the liver and kidney. The protective effects of testosterone or vitamin-E coadministration were also observed

  • Fluoride + aluminum induced toxicity in mice testis witih giant cells and its reversal by vitamin C

    Administration of sodium fluoride (NaF, 10 mg/kg bw) together with aluminium chloride (AlCl3, 200 mg/kg bw) to adult male mice for 30 days resulted in structural alterations in the testis with formation of giant cells. These changes along with lower protein levels affected spermatogenesis. Steroidogenesis was also altered since the

  • Fluoride and aluminum induced toxicity in mice epididymis and its mitigation by vitamin C

    Treatment of adult male mice with sodium fluoride (NaF, 10 mg/kg bw/day) and aluminium chloride (AlCl3, 200 mg/kg bw/day) simultaneously for 30 days caused marked histological changes in the epididymis accompanied by decreased levels of protein and sialic acid and also lowered activities of adenosine triphosphatase (ATPase) and succinate dehydrogenase

  • Investigation of in vitro effects of fluoride on bovine sperm

    The in vitro effects of various concentrations of sodium fluoride on the percentages of motile spermatozoa and abnormal spermatozoa with the hyaluronidase activities of semen were investigated. Holstein bulls (n=20), aged between 4 and 5 years, were used in this study. The semen samples obtained from all animals were divided

  • NADPH oxidase participates in the oxidative damage caused by fluoride in rat spermatozoa. Protective role of a-tocopherol

    Fluorosis, caused by drinking water contaminated with inorganic fluoride, is a public health problem in many areas around the world. The aim of this study was to evaluate oxidative stress in spermatozoa caused by fluoride and NADPH oxidase in relationship to fluoride. Four experimental groups of male Wistar rats were

• Related FAN Content :

  • Fluoride's Effect on Male Reproductive System - Human Studies

    Consistent with in vitro and animal research, studies of human populations have reported associations between fluoride exposure and damage to the male reproductive system. Most notably, a scientist at the Food & Drug Administration reported in 1994 that populations in the United States with more than 3 ppm fluoride in their water had lower "total fertility rates" than populations with lower fluoride levels.

  • Fluoride's Effect on Male Reproductive System: Animal Studies

    Over 60 studies on animals (including rats, mice, roosters, and rabbits) have found that fluoride adversely impacts the male reproductive system. These studies have repeatedly found the following effects: (1) decreases in testosterone levels; (2) reduced sperm motility; (3) altered sperm morphology; (4) reduced sperm quantity; (5) increased oxidative stress; (6) and reduced capacity to breed.

  • Fluoride's Effect on Male Reproductive System -- The "Sprando/Collins" Anomaly

    In contrast to the findings of over 60 animal studies from other research teams, a series of studies by FDA researchers Sprando & Collins reported virtually no evidence of reproductive toxicity among animals treated with very high levels of fluoride exposure. The reasons for this discrepancy remains unclear. Excerpts from Sprando/Collins' Studies: "This study

  • Fluoride's Effect on the Male Reproductive System -- In Vitro Studies

    Carefully controlled in vitro studies have found that direct exposure of fluoride to the testes or semen inhibits testosterone production and damages sperm. While researchers have known since the 1930s that mega concentrations of fluoride can completely (but reversibly) immobilize sperm, it was not until the 1970s and 1980s that researchers found that relatively modest concentrations of fluoride could cause damage prior to complete immobilization.