Abstract

OBJECTIVE:  To observe the effect of sodium fluoride on the expression of steroidogenic acute regulatory protein (StAR) mRNA and cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc) mRNA in the testosterone synthesis of mouse Leydig tumor cells (mLTC-1). and to explore the molecular mechanisms of fluoride on reproductive injury.

METHODS:  Using mLTC-1 cells as a model, progesterone in the supernatant of cell culture medium was measured by RIA. The expression of StAR mRNA and P450scc mRNA in mLTC-1 cells was determined by real-time PCR.

RESULTS:  In comparison with the control group, the expression of StAR mRNA and P450scc mRNA in mLTC-1 cells and the secretion of progesterone of mLTC-1 cells in the three fluoride groups with 12,16 and 20 microg/ml of NaF in their media respectively were obviously lower (P < 0.05).

CONCLUSION:  The expression of StAR mRNA and P450scc mRNA in mLTC-1 cells could be inhibited by NaF, and consequently the secretion of progesterone in mLTC-1 cells was affected.

• Related Studies :

  • Sodium fluoride activates ERK and JNK via induction of oxidative stress to promote apoptosis and impairs ovarian function in rats

    The toxicity of sodium fluoride (NaF) to female fertility is currently recognized; however, the mechanisms are unclear. Previously, we reported a reduction in successful pregnancy rates, ovarian atrophy and dysfunction following exposure to NaF. The purpose of this study was to elucidate the underlying molecular mechanisms. Female Sprague-Dawley rats (10

  • Effects of vitamin C on antioxidant systems and steroidogenic enzymes in sodium fluoride-exposed TM4 sertoli cells

    Fluoride, which is commonly found in drinking water and nutrients, threatens the environment and human life. Fluoride damages cells by causing free radical formation and affects male reproductive function by preventing spermatogenesis, changing the activity of spermatogenetic enzymes, and consequently leading to reduced reproductive capacity. A sufficient amount of antioxidants

  • Protective effects of blackberry and quercetin on sodium fluoride-induced oxidative stress and histological changes in the hepatic, renal, testis and brain tissue of male rat

    BACKGROUND: Sodium fluoride (NaF) intoxication is associated with oxidative stress and altered antioxidant defense mechanism. The present study was carried out to evaluate the potential protective role of blackberry and quercetin (Q) against NaF-induced oxidative stress and histological changes in liver, kidney, testis and brain tissues of rats. METHODS: The rats

  • The toxicity mechanism of sodium fluoride on fertility in female rats

    Recognition of the harmful effects of sodium fluoride (NaF) on human reproduction is increasing, especially as it relates to female reproduction. However, the mechanism by which NaF interferes with female reproduction is unclear. The aims of the present study were to investigate the effects of fluoride exposure on female fertility

  • Abnormal spermatogenesis following sodium fluoride exposure is associated with the downregulation of CREM and ACT in the mouse testis.

    cAMP response element modulator (CREM) is involved in regulating gene expression in normal spermatogenesis. The transcriptional activity of CREM is partly regulated by activator of CREM in the testis (ACT). To investigate the effects of different concentrations of sodium fluoride (NaF) on the gene and protein expression of CREM and

• Related FAN Content :

  • Fluoride's Effect on the Male Reproductive System -- In Vitro Studies

    Carefully controlled in vitro studies have found that direct exposure of fluoride to the testes or semen inhibits testosterone production and damages sperm. While researchers have known since the 1930s that mega concentrations of fluoride can completely (but reversibly) immobilize sperm, it was not until the 1970s and 1980s that researchers found that relatively modest concentrations of fluoride could cause damage prior to complete immobilization.

  • Fluoride's Effect on Male Reproductive System -- The "Sprando/Collins" Anomaly

    In contrast to the findings of over 60 animal studies from other research teams, a series of studies by FDA researchers Sprando & Collins reported virtually no evidence of reproductive toxicity among animals treated with very high levels of fluoride exposure. The reasons for this discrepancy remains unclear. Excerpts from Sprando/Collins' Studies: "This study

  • Fluoride's Effect on Male Reproductive System: Animal Studies

    Over 60 studies on animals (including rats, mice, roosters, and rabbits) have found that fluoride adversely impacts the male reproductive system. These studies have repeatedly found the following effects: (1) decreases in testosterone levels; (2) reduced sperm motility; (3) altered sperm morphology; (4) reduced sperm quantity; (5) increased oxidative stress; (6) and reduced capacity to breed.

  • Fluoride's Effect on Male Reproductive System - Human Studies

    Consistent with in vitro and animal research, studies of human populations have reported associations between fluoride exposure and damage to the male reproductive system. Most notably, a scientist at the Food & Drug Administration reported in 1994 that populations in the United States with more than 3 ppm fluoride in their water had lower "total fertility rates" than populations with lower fluoride levels.