Abstract
OBJECTIVE: To observe the effect of sodium fluoride on the expression of steroidogenic acute regulatory protein (StAR) mRNA and cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc) mRNA in the testosterone synthesis of mouse Leydig tumor cells (mLTC-1). and to explore the molecular mechanisms of fluoride on reproductive injury.
METHODS: Using mLTC-1 cells as a model, progesterone in the supernatant of cell culture medium was measured by RIA. The expression of StAR mRNA and P450scc mRNA in mLTC-1 cells was determined by real-time PCR.
RESULTS: In comparison with the control group, the expression of StAR mRNA and P450scc mRNA in mLTC-1 cells and the secretion of progesterone of mLTC-1 cells in the three fluoride groups with 12,16 and 20 microg/ml of NaF in their media respectively were obviously lower (P < 0.05).
CONCLUSION: The expression of StAR mRNA and P450scc mRNA in mLTC-1 cells could be inhibited by NaF, and consequently the secretion of progesterone in mLTC-1 cells was affected.
-
-
Sodium fluoride induces apoptosis through reactive oxygen species-mediated endoplasmic reticulum stress pathway in Sertoli cells
Excessive fluoride exposure is known to contribute to reproductive system dysfunction, ultimately leading to pathological damage and apoptosis in cells. Although both oxidative and endoplasmic reticulum (ER) stresses have been implicated in fluorosis, the signaling pathways and their roles in sodium fluoride (NaF)-induced apoptosis of Sertoli cells have been sparsely
-
Excessive apoptosis and defective autophagy contribute to developmental testicular toxicity induced by fluoride
Fluoride, a ubiquitous environmental contaminant, is known to impair testicular functions and fertility; however the underlying mechanisms remain obscure. In this study, we used a rat model to mimic human exposure and sought to investigate the roles of apoptosis and autophagy in testicular toxicity of fluoride. Sprague-Dawley rats were developmentally
-
Effects of high fluoride on sperm quality and testicular histology in male rats
Sixty-four forty-day old male Wistar rats were divided randomly into two groups of thirty-two each. With one group untreated as controls, the other group was administered 150 mg NaF/L (68 ppm F–) in their drinking water to assess the effects of high fluoride on sperm quality and testicular histology at
-
Protective effects of blackberry and quercetin on sodium fluoride-induced oxidative stress and histological changes in the hepatic, renal, testis and brain tissue of male rat
BACKGROUND: Sodium fluoride (NaF) intoxication is associated with oxidative stress and altered antioxidant defense mechanism. The present study was carried out to evaluate the potential protective role of blackberry and quercetin (Q) against NaF-induced oxidative stress and histological changes in liver, kidney, testis and brain tissues of rats. METHODS: The rats
-
Effects of sodium fluoride and sulfur dioxide on oxidative stress and antioxidant defenses in rat testes
To assess effects of sodium fluoride and sulfur dioxide on oxidative stress and antioxidant defenses in the testes, 96 sexually mature male Wistar rats were divided randomly into four groups of twenty-four rats each. One group of rats was left untreated as controls, and the other three groups were administered,
Related Studies :
-
-
-
Fluoride's Effect on Male Reproductive System -- The "Sprando/Collins" Anomaly
In contrast to the findings of over 60 animal studies from other research teams, a series of studies by FDA researchers Sprando & Collins reported virtually no evidence of reproductive toxicity among animals treated with very high levels of fluoride exposure. The reasons for this discrepancy remains unclear. Excerpts from Sprando/Collins' Studies: "This study
-
Fluoride's Effect on Male Reproductive System - Human Studies
Consistent with in vitro and animal research, studies of human populations have reported associations between fluoride exposure and damage to the male reproductive system. Most notably, a scientist at the Food & Drug Administration reported in 1994 that populations in the United States with more than 3 ppm fluoride in their water had lower "total fertility rates" than populations with lower fluoride levels.
-
Fluoride's Effect on Male Reproductive System: Animal Studies
Over 60 studies on animals (including rats, mice, roosters, and rabbits) have found that fluoride adversely impacts the male reproductive system. These studies have repeatedly found the following effects: (1) decreases in testosterone levels; (2) reduced sperm motility; (3) altered sperm morphology; (4) reduced sperm quantity; (5) increased oxidative stress; (6) and reduced capacity to breed.
-
Fluoride's Effect on the Male Reproductive System -- In Vitro Studies
Carefully controlled in vitro studies have found that direct exposure of fluoride to the testes or semen inhibits testosterone production and damages sperm. While researchers have known since the 1930s that mega concentrations of fluoride can completely (but reversibly) immobilize sperm, it was not until the 1970s and 1980s that researchers found that relatively modest concentrations of fluoride could cause damage prior to complete immobilization.
Related FAN Content :
-