Abstract

This investigation reports genotoxic effects of sodium fluoride (NaF) at 17, 34, and 51 ?M for 72 hr and induction of sister chromatid exchanges (SCEs) and related changes, together with ameliorative effects by melatonin and amla, in human peripheral blood lymphocyte cell cultures. The cell cycle proliferative index (CCPI) significantly decreased with increasing F concentration. SCEs, average generation time (AGT), and population doubling time (PDT) also significantly increased with F exposure. Treatment with the antioxidants melatonin and amla, separately or in combination with the highest level NaF-treated group, showed a detectable but non- significant increase in CCPI and a decrease in SCEs, AGT, and PDT, comparable to levels in control cultures. The results indicate substantial amelioration of F genotoxicity in lymphocyte cells by melatonin, amla, and their combination.

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