Fluoride Action Network


To explore the effect of fluoride (F) on the expression of purine-rich element-binding protein (PURA) gene and calmodulin (CaM) gene in osteoblasts of newborn rats, parietal calvaria bone osteoblast cultures of 48-hr-old rats were treated for 48 hr with sodium fluoride (NaF) at concentrations of 0 (control), 0.5, 2, and 8 mg/ L. The expression of PURA gene and CaM gene was determined by reverse transcription polymerase chain reaction (R T-PCR). The results indicated that F significantly enhanced (p<0.05 to p<0.01) the expression levels of the two genes in the osteoblast cultures with increasing F concentrations compared with the control group