Various authors have studied the effect of fluoride ion upon mammalian cells using many different biological end-points, of:which inhibition of the function of specific cellular enzymes, survival of cell reproductive capacity, production of abnormalities of cell division and alteration in the rate of cell proliferation are a representative sample. In this review the minimum fluoride ion concentration at which biological effects are detect- able are compared for each of these end-points. The usefulness of such in vitro studies for evaluation of potential whole-animal toxicity is discussed.
Diverse effects of fluoride on NA+ and K+ transport across the rat erythrocyte membrane.
The cellular effects of fluoride (as F – from NaF) on Na + and K + transport across the rat erythrocyte membrane were investigated using 22 Na as a tracer and nonradioactive Rb + as a K + congener. Exposure of rat erythrocytes to 20 mM NaF for 30–120 min
Co-exposure to arsenic and fluoride on oxidative stress, glutathione linked enzymes, biogenic amines and DNA damage in mouse brain.
We studied the effects of combined exposure to arsenic and fluoride on (i) brain biogenic amines, oxidative stress and its correlation with glutathione and linked enzymes; (ii) alterations in the structural integrity of DNA; and (iii) brain and blood arsenic and fluoride levels. Efficacy of alpha-tocopherol in reducing these changes
Influence of extended exposure to sodium fluoride and caffeine on the activity of carbohydrate metabolism enzymes in rat blood serum and liver.
A 50-day study of the influence of fluoride (F) and caffeine on carbohydrate metabolism enzymes was conducted on 18 male Sprague-Dawley rats, 4.5 months old, housed in separate cages at 22–25°C on a 12-hr light/dark cycle. The rats were divided into three groups with six rats in each group: control,
Effect of sodium fluoride ingestion on malondialdehyde concentration and the activity of antioxidant enzymes in rat erythrocytes
Fluoride intoxication has been shown to produce diverse deleterious metabolic alterations within the cell. To determine the effects of sodium fluoride (NaF) treatment on malondialdehyde (MDA) levels and on the activity of antioxidant enzymes in rat erythrocytes, Male Wistar rats were treated with 50 ppm of NaF or were untreated
Dehydrogenase activity in the brain of fluoride and aluminium induced wistar rats
Dehydrogenases are cellular enzymes usually used as indicators of changes in cell activity and morphology; this includes metabolic processes such as structural differentiation, cell migration, cellular damage and even cell death; hence, assay of enzymes as Lactate dehydrogenase and Glucose 6 phosphate dehydrogenase could provide evidence for the role of
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