Abstract

To investigate the effects of sodium fluoride (NaF) on sperm motility, oxidative stress, and apoptosis in the testes, male Wistar rats were exposed to 1.0, 2.0, and 3.0 mg NaF/kg bw/day by intragastric gavage for 90 days. Sperm motility was significantly inhibited, especially at the lower F intake level. Significant increases in oxidative stress with elevated malondialdehyde occurred in the 1.0 mg NaF group and increased hydrogen peroxide in the 2.0 mg NaF group. Compared with the control group without NaF, the cell percentage in G0/G1 phase increased significantly, whereas it decreased significantly in S phase. On the other hand, the percentage of cells in G2/M phase was similar to that of the control. In the 2.0 and 3.0 mg NaF groups, a significant increase in testicular cell apoptosis was observed. Thus, especially at a comparatively lower level of exposure, F exhibits toxic effects on reproductive function in the form of decreased sperm motility, enhanced oxidative stress, and increased apoptosis, although the latter does not appear to be directly connected with the increased level of oxidative stress.

• Related Studies :

  • Toxic effects of sodium fluoride on reproductive function in male mice

    To investigate the effects and possible mechanisms of the action of fluoride on testis cell cycle and cell apoptosis in male mice, sexually mature male Kunming mice were exposed to 50, 100, 200, and 300 mg NaF/L in their drinking water for 8 weeks. At the end of the exposure

  • Selenium may suppress peripheral blood mononuclear cell apoptosis by modulating HSP70 and regulate levels of SIRT1 through reproductive hormone secretion and oxidant stress in women suffering fluorosis.

    Excessive taking fluoride (F) causes severe damage to reproductive system through stimulation of apoptosis and oxidant stress. Selenium (Se) may promote anti-oxidant enzymes and invert cell apoptosis. The aim of this study was to investigate the effect of Se on peripheral blood mononuclear cell (PBMC) apoptosis and oxidant stress in

  • Fluoride-induced oxidative stress and apoptosis are involved in the reducing of oocytes development potential in mice.

    The present study was conducted to investigate the mechanisms of excessive-fluoride-induced reduction of oocyte development potential in mice. The development morphology of oocyte and the changes of pathomorphology in ovary were observed. The protein expression levels of apoptosis factors, including Bax, Bcl-2, casepase-3, casepase-9 and cytochrome c, and the mRNA

  • Positive PCNA and Ki-67 Expression in the Testis Correlates with Spermatogenesis Dysfunction in Fluoride-Treated Rats.

    The present study aimed to evaluate the effect of fluoride (F) on spermatogenesis in male rats. F- at 50 and 100 mg/L was administered for 70 days, after which the testicular and epididymis tissues were collected to observe the histopathological structure under a light microscope. The ultrastructure of the testis and sperm

  • Fluoride-induced apoptosis and gene expression profiling in mice sperm in vivo.

    Exposure to fluoride can induce low sperm quality; however, little is known about the molecular mechanisms by which fluoride exerts its toxic effects. This study was conducted to evaluate ultrastructure, oxidative stress, and apoptosis in sperm of mice treated with 150 mg/l NaF for 49 days. Furthermore, microarray analysis was also utilized

• Related FAN Content :

  • Fluoride's Effect on Male Reproductive System - Human Studies

    Consistent with in vitro and animal research, studies of human populations have reported associations between fluoride exposure and damage to the male reproductive system. Most notably, a scientist at the Food & Drug Administration reported in 1994 that populations in the United States with more than 3 ppm fluoride in their water had lower "total fertility rates" than populations with lower fluoride levels.

  • Nutrient Deficiencies Enhance Fluoride Toxicity

    It has been known since the 1930s that poor nutrition enhances the toxicity of fluoride. As discussed below, nutrient deficiencies have been specifically linked to increased susceptibility to fluoride-induced tooth damage (dental fluorosis), bone damage (osteomalacia), neurotoxicity (reduced intelligence), and mutagenicity. The nutrients of primary importance appear to be calcium,

  • Fluoride & Oxidative Stress

    A vast body of research demonstrates that fluoride exposure increases oxidative stress. Based on this research, it is believed that fluoride-induced oxidative stress is a key mechanism underlying the various toxic effects associated with fluoride exposure. It is also well established that fluoride's toxic effects can be ameliorated by exposure

  • Fluoride's Effect on Male Reproductive System: Animal Studies

    Over 60 studies on animals (including rats, mice, roosters, and rabbits) have found that fluoride adversely impacts the male reproductive system. These studies have repeatedly found the following effects: (1) decreases in testosterone levels; (2) reduced sperm motility; (3) altered sperm morphology; (4) reduced sperm quantity; (5) increased oxidative stress; (6) and reduced capacity to breed.

  • Fluoride content in tea and its relationship with tea quality.

    J Agric Food Chem. 2004 Jul 14;52(14):4472-6. Fluoride content in tea and its relationship with tea quality. Lu Y, Guo WF, Yang XQ. Department of Tea Science, Zhejiang University, 268 Kaixuan Road, Hangzhou 310027, People's Republic of China. Abstract: The tea plant is known as a fluorine accumulator. Fluoride (F) content in fresh leaves collected