Abstract
OBJECTIVE: To study astrocytes cellular cycle and the activities of SDH, 5′-NT and ACP in rat cerebral cortex astrocytes by NaF in vitro.
METHODS: Astrocytes of rat cerebral cortex were purified and cultured in vitro to obtain the highly purified cells (identified by immunochemistry of anti-GFAP). The constituent ratio of cell cycle was analyzed by FCM after the cells were cultured with different levels of NaF for different time span. Activities of SDH, 5′-NT and ACP were tested by UV colorimetry.
RESULTS: Cell cycle arrest transited from S phase to G2/M phase and subG1 cells increased in 1-5 mmol/L in the astrocyte cycle treated with different levels of NaF for different time span by using FCM. There was inverse correlation between the relative amount of the subG1 DNA and activities of SDH and ACP respectively (SDH: r = -0.84148, P < 0.05; ACP: r = -0.90416, P < 0.01) that were inhibited by NaF and 5 mmol/L NaF can inactivate 5′-NT in astrocytes.
CONCLUSION: NaF can induce cell cycle arrest from S to G2/M and inhibit activities of 5′-NT,SDH and ACP in astrocytes.
-
-
Chronic exposure to environmentally relevant concentration of fluoride alters Ogg1 and Rad51 expressions in mice: Involvement of epigenetic regulation.
Highlights Almost 200 million people all over the world are facing dreadful effects of fluoride. Mice were treated orally with environmentally relevant concentration of fluoride for 8 months. Fluoride treatment resulted into histopathology, oxidative stress response and transcriptional alterations of Ogg1 and Rad51. DNA fragmentations and apoptosis induction was
-
Decreased learning and memory ability in rats with fluorosis: increased oxidative stress and reduced cholinesterase activity in the brain
The aim of this research was to study the mechanism of the decreased learning and memory of rats with chronic fluorosis. Compared with controls, decreased learning and memory ability, lower levels of total antioxidant capacity (TAOC), and increased content of malondialdehyde (MDA) in brain tissues were observed in both male
-
Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure.
Background Fluoride (F) is a naturally exists in nature but several studies have indicated it as an environmental toxicant to all leaving beings. Human F exposure has increased over the years since this ion has been used by industry on foods, beverages, toothpastes and on water supply. Although F is safe
-
Studies on the DNA and RNA contents of heart, liver and kidney of rats with chronic fluorosis
17 rats with chronic fluorosis induced by prolonged drinking of water containing 50 ppm fluorine and 17 rats drinking low-fluorine water served as control were used to study the DNA and RNA contents of heart, liver and kidney. The findings suggest that excessive accumulation of fluorine can suppress the synthesis
-
[Effects of selenium and zinc on the DNA damage caused by fluoride in pallium neural cells of rats].
To investigate the effects of fluoride on DNA damage as well as the effects of selenium and zinc against fluoride respectively or jointly in pallium neural cells of rats, single cell gel electrophoresis was used to detect the DNA damage of neural cells prepared in vitro. The results showed that
Related Studies :
-
-
-
Fluoride: Developmental Neurotoxicity.
Developmental Neurotoxicity There has been a tremendous amount of research done on the association of exposure to fluoride with developmental neurotoxicity. There are over 60 studies reporting reduced IQ in children and several on the impaired learning/memory in animals. And there are studies which link fluoride to Attention Deficit Hyperactivity Disorder. Teaching
-
Fluoride & IQ: 71 Studies
As of November 2021, a total of 79 studies have investigated the relationship between fluoride and human intelligence. Of these investigations, 71 studies have found that elevated fluoride exposure is associated with reduced IQ in humans, while over 60 animal studies have found that fluoride exposure impairs the learning and/or
-
NRC (2006): Fluoride's Neurotoxicity and Neurobehavioral Effects
The NRC's analysis on fluoride and the brain.
-
Fluoride Affects Learning & Memory in Animals
An association between elevated fluoride exposure and reduced intelligence has now been observed in 65 IQ studies. Although a link between fluoride and intelligence might initially seem surprising or random, it is actually consistent with a large body of animal research. This animal research includes the following 45 studies (out
-
Fluoride's Direct Effects on Brain: Animal Studies
The possibility that fluoride ingestion may impair intelligence and other indices of neurological function is supported by a vast body of animal research, including over 40 studies that have investigated fluoride's effects on brain quality in animals. As discussed by the National Research Council, the studies have consistently demonstrated that fluoride, at widely varying concentrations, is toxic to the brain.
Related FAN Content :
-