Abstract
OBJECTIVE: To study astrocytes cellular cycle and the activities of SDH, 5′-NT and ACP in rat cerebral cortex astrocytes by NaF in vitro.
METHODS: Astrocytes of rat cerebral cortex were purified and cultured in vitro to obtain the highly purified cells (identified by immunochemistry of anti-GFAP). The constituent ratio of cell cycle was analyzed by FCM after the cells were cultured with different levels of NaF for different time span. Activities of SDH, 5′-NT and ACP were tested by UV colorimetry.
RESULTS: Cell cycle arrest transited from S phase to G2/M phase and subG1 cells increased in 1-5 mmol/L in the astrocyte cycle treated with different levels of NaF for different time span by using FCM. There was inverse correlation between the relative amount of the subG1 DNA and activities of SDH and ACP respectively (SDH: r = -0.84148, P < 0.05; ACP: r = -0.90416, P < 0.01) that were inhibited by NaF and 5 mmol/L NaF can inactivate 5′-NT in astrocytes.
CONCLUSION: NaF can induce cell cycle arrest from S to G2/M and inhibit activities of 5′-NT,SDH and ACP in astrocytes.
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[Study of the mechanism of neurone apoptosis in rats from the chronic fluorosis].
Objective: Study the mechanism of action chronic fluorosis in neurones. Methods: Terminal deoxyribo-nucleotide transferase-mediated dUTP-biotin nick end labeling (TUNEL) and flow cytometry (FCM) were used to observe changes of apoptosis in cerebral cells in chronic fluorosis in rats. Results: TUNEL results show non-random expression of DAB positive stain apoptosis cells which appear
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Genotoxicity of fluoride subacute exposure in rats and selenium intervention.
Highlights Subacute exposure of Wistar rats to fluoride and selenium. Fluoride at a dose of 150 ppm induced DNA damage in the liver, spleen and brain tissue cells, not in blood. Selenium supplementation reduces DNA damage in liver and spleen cells. Fluoride damage did not occur on a histological
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[Studies on DNA damage and apoptosis in rat brain induced by fluoride].
OBJECTIVE: To explore the DNA damage effects and apoptosis in brain cells of rats induced by sodium fluoride. METHODS: SD rats were divided into two groups, i.e. control group and fluoride treated group, which were injected intraperitoneally with distilled water and sodium fluoride (20 mg.kg(-1).d(-1)) respectively. On the hand, 5
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Decreased learning and memory ability in rats with fluorosis: increased oxidative stress and reduced cholinesterase activity in the brain
The aim of this research was to study the mechanism of the decreased learning and memory of rats with chronic fluorosis. Compared with controls, decreased learning and memory ability, lower levels of total antioxidant capacity (TAOC), and increased content of malondialdehyde (MDA) in brain tissues were observed in both male
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Co-exposure to arsenic and fluoride on oxidative stress, glutathione linked enzymes, biogenic amines and DNA damage in mouse brain.
We studied the effects of combined exposure to arsenic and fluoride on (i) brain biogenic amines, oxidative stress and its correlation with glutathione and linked enzymes; (ii) alterations in the structural integrity of DNA; and (iii) brain and blood arsenic and fluoride levels. Efficacy of alpha-tocopherol in reducing these changes
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Fluoride: Developmental Neurotoxicity.
Developmental Neurotoxicity There has been a tremendous amount of research done on the association of exposure to fluoride with developmental neurotoxicity. There are over 60 studies reporting reduced IQ in children and several on the impaired learning/memory in animals. And there are studies which link fluoride to Attention Deficit Hyperactivity Disorder. Teaching
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Fluoride's Direct Effects on Brain: Animal Studies
The possibility that fluoride ingestion may impair intelligence and other indices of neurological function is supported by a vast body of animal research, including over 40 studies that have investigated fluoride's effects on brain quality in animals. As discussed by the National Research Council, the studies have consistently demonstrated that fluoride, at widely varying concentrations, is toxic to the brain.
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Fluoride Affects Learning & Memory in Animals
An association between elevated fluoride exposure and reduced intelligence has now been observed in 65 IQ studies. Although a link between fluoride and intelligence might initially seem surprising or random, it is actually consistent with a large body of animal research. This animal research includes the following 45 studies (out
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Fluoride's Effect on Fetal Brain
The human placenta does not prevent the passage of fluoride from a pregnant mother's bloodstream to the fetus. As a result, a fetus can be harmed by fluoride ingested pregnancy. Based on research from China, the fetal brain is one of the organs susceptible to fluoride poisoning. As highlighted by the excerpts
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NRC (2006): Fluoride's Neurotoxicity and Neurobehavioral Effects
The NRC's analysis on fluoride and the brain.
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