Abstract

Nettle, Urtica dioica L., is frequently used by humans for medicinal purposes and has antioxidant, anti-inflammatory, and hepatoprotective properties. As no data were available on the effect of nettle extract on the synthesis of pro-inflammatory agents in hepatocytes treated with fluoride, we decided to investigate this. Aqueous and ethanol extracts of nettle fruit clusters were prepared, after lyophilisation and homogenisation, and added to hepatocytes from HepG2 cell line, with and without NaF. After incubation, the concentrations of the pro-inflammatory end products of 15-LOX activity in hepatocytes (15-HETE, 12-HETE, 9-HODE, and 13-HODE) were determined by high performance liquid chromatography (HPLC), and apoptosis, necrosis, and mitochondrial superoxide generation were measured using immunohistochemical methods. Adding NaF increased the 15-LOX proinflammatory end products and these effects were decreased by both the ethanol and aqueous nettle extracts. Similarly, the ethanol extracts reduced the increased apoptosis, necrosis, and mitochondrial superoxide synthesis caused by NaF suggesting that nettle extracts may have hepatoprotective properties in fluoride intoxication

• Related Studies :

  • Challenges of fluoride pollution in environment: Mechanisms and pathological significance of toxicity – A review.

    Highlights Fluoride exposure induces mitochondrial function dysfunction. Fluoride exposure mediates organs injury involved in apoptosis and autophagy. Fluoride exposure induces inflammatory reaction. Fluoride exposure results changes in intestinal microbial abundance. Fluoride is an important trace element in the living body. A suitable amount of fluoride has a beneficial effect on

  • Drp1/Mff signaling pathway is involved in fluoride-induced abnormal fission of hepatocyte mitochondria in mice.

    Highlights Fluoride induced histological changes in the liver tissue. Fluoride induced significant damage to hepatocyte in mice. Fluoride caused notable mitochondrial morphological structure change. Drp1/Mff signaling pathway was involved in fluoride-induced mitochondria division. Fluoride induced apoptosis in hepatocyte. Fluoride, a toxic substance, is widely distributed in the environment and causes

  • Exposure to Fluoride induces apoptosis in liver of ducks by regulating Cyt-C/Caspase 3/9 signaling pathway.

    Highlights Long-term exposure NaF induced hepatotoxicity in ducks. NaF exposure caused liver granule and vacuolar degeneration observed by histological analysis. The mechanism of NaF exposure on hepatotoxicity involving activating autophagy and apoptosis. Cyt-C/Caspase 3/9 signaling pathway activation target cell death. Abstract Fluorine being a well-known and essential element for normal physiological

  • Significance of Inflammation and Apoptosis in Hepatocellular Death in Rat, Co-treated with Arsenic and Fluoride.

    Health effects elicited by combined environmental exposures to xenobiotics, in many instances, still remain unresolved. One of these examples is the combined toxicity of arsenic and fluoride. The present study was undertaken to delineate the role of inflammation and apoptosis in hepatocellular death caused by co-exposure to arsenic and fluoride

  • Proposed mechanism for understanding the dose- and time-dependency of the effects of fluoride in the liver.

    Fluoride (F) can induce changes in the expression of several liver proteins.It is suggested that these changes are dose- and time-dependent. The objective of this study was to analyze the effect of different F concentrations and exposure times to this ion on the pattern of protein expression in the liver

• Related FAN Content :

  • Does Fluoride Ingestion Affect Developing Immune System Cells?

    Considerations, supported by some published experimental evidence, suggest that fluoride released during the resorption of high-fluoride bone may produce detrimental effects not only on bone cells but on developing cells of the immune system.

  • Is the Ingestion of Fluoride an Immunosuppressive Practice?

    This paper records several observations which suggest that habitual ingestion of small doses of fluoride, even as small as the 1 mg/L contained in fluoridated water, may decrease the function of the immune system.

  • Fluoride & the Immune System - Summation from the US National Research Council (2006)

    “There is no question that fluoride can affect the cells involved in providing immune responses. The question is what proportion, if any, of the population consuming drinking water containing fluoride at 4.0 mg/L on a regular basis will have their immune systems compromised? Not a single epidemiologic study has investigated whether fluoride in the drinking water at 4 mg/L is associated with changes in immune function. Nor has any study examined whether a person with an immunodeficiency disease can tolerate fluoride ingestion from drinking water.”