Fluoride is widely distributed in nature and a direct source of adverse health effects in human populations. Fluoride poisoning attributed by long-term exposure to high levels of fluoride [is] called fluorosis. The present study was carried out among 9-14 years old school children of Dausa district, Rajasthan India. The subjects were selected from high fluoride region (>2.0 ppm) and age matched were selected from Jaipur district where fluoride content was (<1.5 ppm). A [questionnaire] was used to collect information from the children’s personal character, clinical health outcome, residential record, educational achievements and family socioeconomic status. Fluoride in serum, urine and drinking water were estimated in both subjects and controls along with serum vitamin (A, C, D and E) and acetylcholinesterase (AChE). The Raven’s Standard Progressive Matrices was used to measurement of children’s intelligence. IQ scores and serum F levels was directly correlated with the concentration of serum F level. Reduced concentration of vitamin (C, D and E) and AChE activity is the potent markers of neuro-degeneration. The conclusion of the study is that the excessive fluoride delineates the neuronal impairment which were evident by reduce IQ score and serum AChE activity. Moreover, the altered vitamin concentrations which may further lead to brain and bone damage.