The present study was undertaken to evaluate cardio protective effect of rutin against sodium fluoride-induced oxidative stress mediated cardio toxicity and blood toxicity. Cardiac injury was induced by daily administration of sodium fluoride 600ppm in distilled water for 4 weeks. The animals exposed to NaF exhibited a significant increase in levels of cardiac serum markers, lipid peroxidative markers, serum total cholesterol, LDL, triglycerides and decrease in HDL levels. Decrease in haematological parameters viz. haemoglobin, red blood cells, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin count and increase in white blood cells, erythrocyte sedimentation levels was also observed. Marked histopathological lesions, increased DNA fragmentation in cardiac tissues was observed. Activity of antioxidants-catalase, superoxide dismutase, reduced glutathione contents were decreased (P<0.01) whereas lipid peroxidation product (MDA) was increased. A significant decrease in body and heart weight was also observed. Treatment with rutin effectively ameliorated the alterations in the studied parameters of rat through its antioxidant nature. There was also significant improvement in haematological parameters. Thus, results of the present study clearly demonstrated that treatment with rutin against NaF intoxication has a significant role in protecting F-induced cardiotoxicity, blood toxicity and dyslipidemia in rats.