Abstract

Fluoride and aluminium have been reported to cause severe alterations in the brain. However, their exact mechanisms of neurotoxic activities remain unknown.

AIM: This study was designed to investigate the role of fluoride and aluminium in neuronal transport, lysosomal, cell cycle protein and acetylcholinesterase activities.

METHOD: Adult Wistar rats were given low and high doses of fluoride, aluminium and a combination of both with the control group receiving distilled water for 30 days. Blood sera and brain homogenates were quantified for alkaline phosphatase (biomarker for neuronal transport) activities. Brain sections were stained with cresyl fast violet to detect neuronal cell damage. Histochemical demonstration of acetylcholinesterase (AChE) activity and the immunohistochemical detection of cell cycle protein (anti-cyclin D) and lysosomal protein (anti-cathepsin D) were done using the antigen retrieval method.

RESULT: Results showed severe histomorphologic alterations, dysregulation of membrane transport activities, inhibition of AChE activities and increased expression of lysosomal and cell cycle proteins.

CONCLUSION: These findings confirm that excessive fluoride and aluminium intake induces the progression of cell death which inhibit AChE activities and trigger the release of lysosomal and cell cycle proteins.

• Related Studies :

  • Pathologic changes and effect on the learning and memory ability in rats exposed to fluoride and aluminum

    Background: The aim of this study is to establish a single and combined intoxication model of fluoride and aluminum so as to observe the impact of these chemicals on the learning and memory ability and the pathologic changes in brain of rats. Methods: Forty male Wistar rats were randomly assigned

  • [Effects of high fluoride and low Iodine on learning-memory and TchE of brain in offspring rats].

    Objective To study the effect of high level fluoride and low level iodine on learning-memory in offspring rats and possible mechanism. Methods Thirty-two Wistar rats were randomly divided into four groups each of eight (female:male=(3:1).) The rats were treated with high fluoride (100 and 150 mg NaF/L), low iodine (0.0855 mg/kg),

  • Effect of concurrent chronic exposure of fluoride and aluminum on rat brain.

    The present in vivo study was designed to investigate the toxic potential of fluoride alone and in conjugation with aluminum on the rat brain. The region-specific response of both elements was studied in different regions of brain, namely the cerebrum, cerebellum, and medulla oblongata. Following fluoride exposure, oxidative stress increased

  • Chronic Fluoride Exposure and the Risk of Autism Spectrum Disorder.

    The continuous rise of autism spectrum disorder (ASD) prevalent in the past few decades is causing an increase in public health and socioeconomic concern. A consensus suggests the involvement of both genetic and environmental factors in the ASD etiopathogenesis. Fluoride (F) is rarely recognized among the environmental risk factors of

  • Alterations in plasma and tissue acetylcholinesterase activity following repeated oral exposure of chlorpyrifos alone and in conjunction with fluoride in Wistar Rats

    Concurrent exposures of more than one environmental contaminants are commonly encountered by human beings and animals. This study investigated the effect of chlorpyrifos alone and in conjunction with fluoride on plasma and tissue acetylcholinesterase (AChE) activity in wistar rats. Fluoride at 1 or 10 ppm in drinking water produced significant (P < 0.05)

• Related FAN Content :

  • Fluoride's Direct Effects on Brain: Animal Studies

    The possibility that fluoride ingestion may impair intelligence and other indices of neurological function is supported by a vast body of animal research, including over 40 studies that have investigated fluoride's effects on brain quality in animals. As discussed by the National Research Council, the studies have consistently demonstrated that fluoride, at widely varying concentrations, is toxic to the brain.

  • Fluoride Affects Learning & Memory in Animals

    An association between elevated fluoride exposure and reduced intelligence has now been observed in 65 IQ studies. Although a link between fluoride and intelligence might initially seem surprising or random, it is actually consistent with a large body of animal research. This animal research includes the following 45 studies (out

  • Fluoride: Developmental Neurotoxicity.

    Developmental Neurotoxicity There has been a tremendous amount of research done on the association of exposure to fluoride with developmental neurotoxicity. There are over 60 studies reporting reduced IQ in children and several on the impaired learning/memory in animals. And there are studies which link fluoride to Attention Deficit Hyperactivity Disorder. Teaching

  • NRC (2006): Fluoride's Neurotoxicity and Neurobehavioral Effects

    The NRC's analysis on fluoride and the brain.

  • Fluoride & IQ: 67 Studies

    As of May 2020, a total of 75 studies have investigated the relationship between fluoride and human intelligence. Of these investigations, 67 studies have found that elevated fluoride exposure is associated with reduced IQ in humans, while over 60 animal studies have found that fluoride exposure impairs the learning and/or