Abstract
PURPOSE: To study the effect of fluoride on autophagy in rat ameloblasts both in vitro and in vivo.
METHODS: Logarithmic-phase HAT-7 cells were cultured in different concentrations of fluoride for 48h. Transmission electron microscopy (TEM) was used to detect autophagosomes. Western blot and RT-qPCR were carried out to examine the expression of LC3 and Beclin 1. Forty Wistar rats were divided into 4 groups randomly. The expression of LC3 and Beclin 1 in rats was investigated by immunohistochemical staining in vivo. The data were analysed using SPSS13.0 software package.
RESULTS: The amount of autophagosomes in the experimental group was significantly more than that in the control group (P<0.05). The expression of LC3 and Beclin 1 were up-regulated in dose dependent manner after treatment with fluoride. Regression analysis showed that fluoride dependently induced the expression of LC3 and Beclin 1 (P<0.05).Immunohistochemical analysis revealed that the expression of LC3 and Beclin 1 in rat ameloblasts in the experimental groups was positive compared to control group.
CONCLUSIONS: Excessive fluoride induced autophagy in ameloblasts both in vitro and in vivo.
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Short exposure to high levels of fluoride induces stage-dependent structural changes in ameloblasts and enamel mineralization.
We tested the hypothesis that the sensitivity of forming dental enamel to fluoride (F-) is ameloblast developmental stage-dependent and that enamel mineralization disturbances at the surface of fluorotic enamel are caused by damage to late-secretory- and transitional-stage ameloblasts. Four-day-old hamsters received a single intraperitoneal dose of 2.5-20 mg NaF/kg body
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Effect of fluoride ions on apatite crystal formation in rat hard tissues.
Fluoride is widely believed to be a useful chemical substance for preventing dental caries. However, the mechanism underlying crystal perforation in the tooth enamel and the effect of fluoride on hard tissues are unclear. To clarify the mechanism of the biological action of fluoride in the mineralization process, we examined
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Evaluation of genetic polymorphisms in MMP2, MMP9 and MMP20 in Brazilian children with dental fluorosis.
Highlights MMP2, MMP9 and MMP20 were expressed in the enamel development of the animalmodels. Polymorphisms in MMP2, MMP9 and MMP2 were not associated with dental fluorosis. Afro-descendants had a higher risk of dental fluorosis than caucasian. Recent studies suggested that genetics contribute to differences in dental fluorosis (DF) susceptibility among individuals
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Fluoride-induced ultrastructural changes in exocrine pancreas cells of rats: fluoride disrupts the export of zymogens from the rough endoplasmic reticulum (rER).
Influence of fluoride on exocrine pancreas cells was examined morphologically with traditional and prolonged osmium fixation techniques for electron microscopy in the enamel fluorosis model rats injected subcutaneously twice a day with 20 mg/kg body weight of sodium fluoride. Although the rough endoplasmic reticulum (rER) of exocrine pancreas cells in
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Fluoride induced endoplasmic reticulum stress and calcium overload in ameloblasts
OBJECTIVE: The aim of the study was to evaluate the involvement of endoplasmic reticulum stress and intracellular calcium overload on the development of dental fluorosis. METHODS: We cultured and exposed rat ameloblast HAT-7 cells to various concentrations of fluoride and measured apoptosis with flow cytometry and intracellular Ca2+ changes using confocal
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