The objective of this study was to determine the fluoride content in drinking water and urine samples of adolescent males aged 11–16 years living in Southern Haryana, India. A total of 30 drinking water sources in the studied habitations were assessed for fluoride contamination. Fluoride was estimated in the urine of 400 male children randomly selected from these habitations. The fluoride concentration in drinking water and urine samples was determined using USEPA fluoride ion selective electrode method. The mean fluoride concentration in drinking water samples of Pataudi, Haily Mandi and Harsaru villages was 1.68Â ±Â 0.35, 3.22Â ±Â 1.18 and 1.78Â ±Â 0.12Â mg/l, respectively. The mean urinary fluoride concentration was 2.26Â ±Â 0.024Â mg/l at Pataudi, 2.48Â ±Â 0.77Â mg/l at Haily Mandi and 2.43Â ±Â 0.84Â mg/l at Harsaru village. The higher fluoride levels in the urine of children may be associated to higher fluoride levels in drinking water. The accuracy of measurements was assessed with known addition method in water and urine. Mean fluoride recovery was 98.0 and 99.1% in water and urine. The levels obtained were reproducible with in ±3% error limit.