- NaF enhances intracellular generation of ROS and RNS in human RBC.
- It increases oxidation of proteins, thiols and lipids.
- NaF inhibits antioxidant enzymes and lowers antioxidant power of RBC.
- Antioxidant 3,4-dihydroxybenzaldehyde mitigates NaF-induced oxidative damage in RBC.
Fluoride is an essential micronutrient that is needed for mineralization of bones and formation of dental enamel. It is a widely dispersed environmental pollutant and chronic exposure to it is toxic, resulting in malignancies and hematological damage in humans. Blood is a major and early target of environmental pollutants and toxicants like fluoride. Fluoride generates reactive oxygen species and free radicals which induce oxidative stress in target cells and mediate its toxic effects. The aim of this study was to determine the mitigating effect of plant antioxidant 3,4-dihydroxybenzaldehyde (DHB) on sodium fluoride (NaF) induced oxidative damage and cytotoxicity in isolated human red blood cells (RBC)
Isolated human RBC were treated with 0.5 mM NaF, in absence or presence of different concentrations of DHB (0.1–2.5 mM). Several biochemical parameters were analyzed in cell lysates and whole cells.
Treatment of RBC with NaF increased the formation of reactive oxygen and nitrogen species. It oxidized thiols, proteins and lipids and generated their peroxidative products. Methemoglobin level, heme degradation and lipid peroxidation were increased but cellular antioxidant status declined significantly in NaF alone treated RBC, compared to the control. NaF inhibited antioxidant, membrane bound and glycolytic enzymes in RBC. However, prior incubation of RBC with DHB significantly attenuated the NaF-induced alterations in all these parameters in a DHB concentration-dependent manner.
These results show that DHB mitigates NaF-induced oxidative damage in human RBC, probably because of its antioxidant character.
*Original abstract online at https://www.sciencedirect.com/science/article/pii/S0946672X21001784
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Fluoride induces oxidative stress and ATP depletion in the rat erythrocytes in vitro
The present study was designed to examine an ability of inorganic fluoride (F) to induce oxidative stress and energy depletion in the rat erythrocytes in vitro. Accumulation of ROS and alterations in glutathione (GSH) and ATP contents were estimated in the cells incubated with 0.1-10mM NaF for 1, 5 and
Protective effect of resveratrol on sodium fluoride-induced oxidative stress, hepatotoxicity and neurotoxicity in rats
Protective effect of resveratrol on sodium fluoride-induced oxidative stress, hepatotoxicity and neurotoxicity were studied in rats. A total of 28 Wistar albino male rats were used. Four study groups were randomly formed with seven animals in each. The groups were treated for 21 days with distilled water (control group), with
Rutin potentially attenuates fluoride induced oxidative stress mediated cardiotoxicity, blood toxicity and dyslipidemia in rats
The present study was undertaken to evaluate cardio protective effect of rutin against sodium fluoride-induced oxidative stress mediated cardio toxicity and blood toxicity. Cardiac injury was induced by daily administration of sodium fluoride 600ppm in distilled water for 4 weeks. The animals exposed to NaF exhibited a significant increase in
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In the village of Kheru Nayak Thanda in the Gulbarga district of Karnataka, India, 18 children aged 3 to 10 years with endemic skeletal fluorosis were shown to have oxidative stress as evidenced by elevated levels of malondialdehyde in their red blood cells, indicating increased lipid peroxidation. Significant alterations of
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