Aloe vera protects against fluoride-induced teratogenic effects during pre- and postnatal development in mice.

References Aboubakr M, Elbadawy M, Soliman A, Hewaity M (2014) Embryotoxic and teratogenic effect of norfloxacin in female albino rats. Adv Pharmacol Pharmaceutical Sci 2014:1–6. https://doi.org/10.1155/2014/924706 CAS Article Google Scholar Anuradha CD, Kanno S, Hirano S (2001) Oxidative damage to mitochondria is a preliminary step to caspase-3 activation in fluoride-induced apoptosis in HL-60cells. Free Rad Biol Med 31(3):367–373. https://doi.org/10.1016/s0891-5

Abstract

Pregnancy and feto-gestational toxicities on exposure to fluoride and its possible amelioration on co-administration with aloe vera were studied in pregnant Swiss albino mice. Once the confirmed pregnancy was tested, animals were equally divided into four groups as follows: group I was given no treatment and served as control, and groups II and III were administered with 100 and 300 ppm sodium fluoride, respectively, while group IV was co- administered aloe vera (300 mg/kg bw) along with sodium fluoride (300 ppm) daily for 14 days prior to gestation and continued till the 18th day of gestation. Animals were sacrificed on the 19th day of gestation for prenatal observations. Maternal body weight, the gravid uterine weight, number of corpora lutea in both the ovaries, number of implantations and resorptions, number of live (mature and immature) fetuses, and number of dead fetuses were examined in each dam. The treatment continued in another set of animals till the completion of the weaning period to observe postnatal changes due to test substances on the mother and pups. Sodium fluoride–treated animals showed morphometric and skeletal changes which were more pronounced in the high-dose group showing significantly decreased body weight gain in pregnant mothers and dead/immature fetuses. Morphometric changes included open eyelids, limb defects, wrinkles on the whole body, anophthalmia, pulmonary edema, enlarged esophagus, and decreased body weight of fetuses and pups. Alizarin-prepared skeletal structures of fetuses of such female mice showed delayed ossification or bending in the number of bones of skull, thoracic, and limb regions. However, concomitant exposure to sodium fluoride and aloe vera in treated animals led to a marked improvement in all the prenatal and postnatal variables. The study suggests that sodium fluoride at high concentrations may be teratogenic while co-administration of aloe vera during fluoride exposure might be beneficial in reducing these toxic effects. The use of aloe vera as a preventive agent or as a complimentary agent is thus recommended following fluoride exposure through the oral route.

Keywords: Aloe vera; Ameliorative effect; Skeletal deformities; Sodium fluoride; Teratogenicity.

*Original abstract online at https://pubmed.ncbi.nlm.nih.gov/35460489/

Excerpt:

References

Aboubakr M, Elbadawy M, Soliman A, Hewaity M (2014) Embryotoxic and teratogenic effect of norfloxacin in female albino rats. Adv Pharmacol Pharmaceutical Sci 2014:1–6. https://doi.org/10.1155/2014/924706

CAS Article Google Scholar
Anuradha CD, Kanno S, Hirano S (2001) Oxidative damage to mitochondria is a preliminary step to caspase-3 activation in fluoride-induced apoptosis in HL-60cells. Free Rad Biol Med 31(3):367–373. https://doi.org/10.1016/s0891-5849(01)00591-3

CAS Article Google Scholar
Atik N, Nandika A, Cyntia Dewi PI, Avriyanti E (2019) Molecular mechanism of aloe barbadensis miller as a potential herbal medicine. Systematic Reviews in Pharmacy 10(1):118–125. https://doi.org/10.5530/srp.2019.1.20

CAS Article Google Scholar
Barbier O, Arreola-Mendoza L, DelRazo LM (2010) Molecular mechanisms of fluoride toxicity. Chem Biol Interact 188(2):319–333. https://doi.org/10.1016/j.cbi.2010.07.011

CAS Article Google Scholar
Brittany L, Poulev A, Kuhn P, Mary GH, Lila M, Raskin I (2014) Quinoa seeds leach phytoecdysteroids and other compounds with anti-diabetic properties. Food Chem 15(163):178–185. https://doi.org/10.1016/j.foodchem.2014.04.088

CAS Article Google Scholar
Caldera R, Chavinie J, Fermanian J, Tortrat D, Laurent AM (1988) Maternal-fetal transfer of fluoride in pregnant women. Biol Neonate 54(5):263–269. https://doi.org/10.1159/000242861

CAS Article Google Scholar
Callaghan J, Brown S, Battcock T, Parry S, Snook J (2012) Aggressive Helicobacterpylori- negative peptic ulceration as the initial manifestation of Crohn’s disease. Frontline Gastroenterol 3(3):201–205. https://doi.org/10.1136/flgastro-2012-100126

Article Google Scholar
Chlubek D, Grucka-Mamczar E, Birkner E, Polaniak R, Stawiarska-Pi?ta B, Duliban H (2003) Activity of pancreatic antioxidative enzymes and malondialdehyde concentrations in rats with hyper-glycemia caused by fluoride intoxication. J Trace Elem Med Bio 17(1):57–60. https://doi.org/10.1016/s0946-672x(03)80047-0

CAS Article Google Scholar
Choudhary S, Mathur P (2020) Assessment of protective role of quinoa against sodium fluoride induced skeletal deformities in mice fetuses. Res J Pharmacy Technol 13(5):2129–2134. https://doi.org/10.5958/0974-360X.2020.00383.2

Article Google Scholar
Dharani B, Sumathi S, Sivaprabha J and Padma PR (2011) In vitro antioxidant potential of Prosopis cineraria leaves. J Nat Product Plant Resour 1(3): 26–32. https://www.scholarsresearchlibrary.com/articles/in-vitro-antioxidant-potential-of-prosopis-cineraria-leaves.pdf
EFSA (2005). Opinion of the scientific panel on dietetic products, nutrition and allergies on a request from the commission related to the tolerable upper intake level of fluoride (Request No 331 EFSA-Q-2003–018). EFSA J 192, 1–65. https://efsa.onlinelibrary.wiley.com/doi/pdf/10.2903/j.efsa.2005.233
Ekstrand J, Alvan G, Boreus LO, Norlin A (1977) Pharmacokinetics of fluoride in man after single and multiple oral doses. Eur J Clin Pharmacol 12(4):311–317. https://doi.org/10.1007/bf00607432

CAS Article Google Scholar
Elshama S, Abdalla M.E. and Mohamed AM (2018) Role of natural antioxidants in treatment of toxicity. J Analytical Toxicol 1(3), 59–69. https://www.imedpub.com/articles/role-of-natural-antioxidants-in-treatment-of-toxicity.php?aid=23195
Eshun K, He Q (2004) Aloe vera: a valuable ingredient for the food, pharmaceutical and cosmetic industries – a review. Crit Rev Food Sci Nutr 44(5):91–98. https://doi.org/10.1080/10408690490424694

Article Google Scholar
Flora SJS, Shrivastava R, Mittal M (2013) Chemistry and pharmacological properties of some natural and synthetic antioxidants for heavy metal toxicity. Current Medicinal Chemistr 20(36):4540–4574. https://doi.org/10.2174/09298673113209990146

CAS Article Google Scholar
Garcia-Montalvo EA, Reyes-Perez H, Del Razo LM (2009) Fluoride exposure impairs glucose tolerance via decreased insulin expression and oxidative stress. Toxicology 263(3):75–83. https://doi.org/10.1016/j.tox.2009.06.008 (348)

CAS Article Google Scholar
Godoy D, Chokboribal J, Pauwels R, Banlunara W, Sangvanich P, Jaroenporn S, Thunyakitpisal P (2018) Acemannan increased bone surface, bone volume, and bone density in a calvarial defect model in skeletally-mature rats. J Dent Sci 13(1):334–341. https://doi.org/10.1016/j.jds.2018.06.004 (352)

Article Google Scholar
Guth S, Huser S, Roth A, Degen G, Diel P, Edlund K (2020) Toxicity of fluoride: critical evaluation of evidence for human developmental neurotoxicity in epidemiological studies, animal experiments and in vitro analyses. Arch Toxicol 94(3):1375–1415. https://doi.org/10.1007/s00204-020-02725-2

CAS Article Google Scholar
Gutknecht J, Walter A (1981) Hydrofuoric and nitric acid transport through lipid bilayer membranes. Biochem Biophys Acta 644(1):153–156. https://doi.org/10.1016/0005-3592736(81)90071-7

CAS Article Google Scholar
Hull DH, Beale PJ (1985) Cigarette Smoking and Duodenal Ulcer. Gut 26(12):1333–1337. https://doi.org/10.1136/gut.26.12.1333

CAS Article Google Scholar
Hussein H.H. and Mahmoud O.M. (2013) Effects of maternal administration of aluminum chloride on the development of the skeletal system of albino rat fetuses- protective role of saffron. European Journal of Anatomy 17(2), 63–71. https://www.eurjanat.com/v1/journal/pdfdownload.php?id=120198om
Inamdar I.F., Aswar N.R., Sonkar V.K. and Doibale M.K. (2012) Drug utilization pattern during pregnancy. Indian Medical Gazette 23(2), 305–311. https://pubmed.ncbi.nlm.nih.gov/16864912/
Izquierdo-Vega JA, Sanchez-Gutierrez M, DelRazo LM (2008) Decreased in vitro fertility in male rats exposed to fluoride-induced oxidative stress damage and mitochondrial transmembrane potential loss. Toxicol Appl Pharmacol 230(3):352–357. https://doi.org/10.1016/j.taap.2008.03.008

CAS Article Google Scholar
Jing Li, Li Yao A, Qing-Liang Shao A, Chun-Yan Wuab A and Daqing (2004) Effects of high fluoride level on neonatal neurobehavioral development. Fluoride 41(2), 165–170. http://www.fluorideresearch.org/412/files/FJ2008_v41_n2_p165-170.pdf
Lee JH, Jung JY, Jeong YJ et al (2008) Involvement of both mitochondrial and death receptor dependent apoptotic pathways regulated by Bcl-2 family in sodium fluoride-induced apoptosis of thehuman gingival fibroblasts. Toxicology 243(3):340–347. https://doi.org/10.1016/j.tox.2007.10.026

CAS Article Google Scholar
Lee, YB, Yu, J, Choi, HH, Bu S, Kim, H K, Kim, S, Kim, SS, Yong G (2017) The association between peptic ulcer diseases and mental health problems: a population –based study: a STROBE compliant article. Medicine 96(34). https://doi.org/10.1097/md.0000000000007828
Madhusudhan N, Basha M.P., Rai P, Ahmed F and Prasad RG (2009) Effect of maternal Fluoride exposure on developing CNS of rats: protective role of aloe vera, Curcuma longa and Ocimum Sanctum. Indian J Exp Biol 48(3): 830–836. http://nopr.niscair.res.in/bitstream/123456789/9989/1/IJEB%2048%288%29%20830-836.pdf
Madu EP (2015) Teratogenic and embryo toxic effects of orally administered cypermethrin in pregnant albino rats. J Toxicol Environ Health Sci 7(7):60–67. https://doi.org/10.5897/JTEHS2015.0336 (388)

CAS Article Google Scholar
Mendoza-Schulz A, Solano-Agama C, Arreola-Mendoza L (2009) The effects of fluoride on cell migration, cell proliferation, and cell metabolism in GH4C1 pituitary tumor cells. Toxicol Lett 90(2):179–186. https://doi.org/10.1016/j.toxlet.2009.07.014

CAS Article Google Scholar
Munoth P, Tiwari K and Goyal R (2015) Fluoride and nitrate groundwater contamination in Rajasthan, India: A Review. 20^th international conference on hydraulics, water resources and river engineering, IIT Roorkee, India. https://doi.org/10.13140/RG.2.1.2859.6241
Nada AS, Hawas AM, AbdElmageed ZY, Amin NE (2013) Protective value of aloe vera extract against g-irradiation induced some biochemical disorders in rats. J Radiat Res Appl Sci 6(1):31–37. https://doi.org/10.1016/j.jrras.2013.10.003

CAS Article Google Scholar
Narayanaswamy M, Basha Piler M (2010) Effect of maternal exposure of fluoride on biometals and oxidative stress parameters in developing CNS of rat. Biol Trace Elem Res 133(2):71–82. https://doi.org/10.1007/s12011-009-8413-y

CAS Article Google Scholar
Ozsvath D (2008) Fluoride and environmental health: a review. Review in Environmental Science and Bio/technology 8(1):59–79. https://doi.org/10.1007/s11157-008-9136-9

CAS Article Google Scholar
Osefo N, Ito T, Jensen RT (2009) Gastric acid hypersecretory states: recent insights and advances. Curr Gastroenterol Rep 11(6):433–441. https://doi.org/10.1007/s11894-009-0067-6

Article Google Scholar
Panchal S, Verma RJ (2014) Effect of Sodium fluoride in maternal and offspring rats and its amelioration. Asian Pacific Journal of Reproduction 3(1):71–76. https://doi.org/10.1016/S2305-0500(14)60005-1

Article Google Scholar
Perumal E, Paul V, Govindrajan V, Panneerselvam L (2013) A brief review on experimental fluorosis. Toxicol Lett 223(2):236–251. https://doi.org/10.1016/j.toxlet.2013.09.005

CAS Article Google Scholar
Rauws EAJ, Tytgat GNJ (1990) Cure of duodenal ulcer associated with eradication of Helicobacterpylori. Lancet 335(2):1233–1235. https://doi.org/10.1016/0140-6736(90)91301-P

CAS Article Google Scholar
Shastry BV (1999) Techniques to study human placental transport. Adv Drug Deliv Rev 38(6):17–39. https://doi.org/10.1016/s0169-409x(99)00004-6

Article Google Scholar
Shen YW, Taves DR (1974) Fluoride concentrations in the human placenta and maternal and cord blood. Am J ObstetGynecol 119(2):205–207. https://doi.org/10.1016/0002-9378(74)90035-0

CAS Article Google Scholar
Singh RH (2000) Positive health potentialsof Rasayan-Vajikaran therapy in Ayurveda. Ayurmedline 2(3): 1–9. https://ayurvedline.in/2020/08/16/positive-health-potential-of-rasayana-vajikarana-therapy-in-ayurveda/
Sun Z, Zhang Y, Xue X, Niu R, Wang J (2018) Maternal fluoride exposure during gestation and location decreased learning and memory ability of mouse pups. Hum Exp Toxicol 37(1):87–93. https://doi.org/10.1177/0960327117693067

CAS Article Google Scholar
Vinson J, Kharrat AH, Andreoli L (2005) Effect of aloe vera preparations on the human bioavailability of vitamins C and E. Phytomedicine 12(10):760–769. https://doi.org/10.1016/j.phymed.2003.12.013

CAS Article Google Scholar
Wai KM, Mar O, Kosaka S, Umemura M, Watanabe C (2017) Prenatal heavy metal exposure and adverse birth outcomes in myanmar: a birth – cohort study. Int J Environ Res Public Health 14(11):1339. https://doi.org/10.3390/ijerph14111339

CAS Article Google Scholar
Whitford GM, Sampaio FC, Pinto CS, Maria AG, Cardoso VE, Buzalaf MA (2008) Pharmacokinetics of ingested fluoride: lack of effect of chemical compound. Arch Oral Biol 53(11):1037–1041. https://doi.org/10.1016/j.archoralbio.2008.04.001

CAS Article Google Scholar

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Aloe vera protects against fluoride-induced teratogenic effects during pre- and postnatal development in mice.

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