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Combined Effects of Fluoride and Dietary Seleno-L-Methionine at Environmentally Relevant Concentrations on Female Zebrafish (Danio rerio) Liver: Histopathological Damages, Oxidative Stress and Inflammation.
| Biological Trace Element Research | Zhang X, Cao J, Chen J, Wang G, Li L, Wei X, Zhang R. | 202(5):2314-2326.
Protective effect Selenium Zebrafish Liver Oxidative Stress Animal Study

Abstract

Original abstract online at
https://link.springer.com/article/10.1007/s12011-023-03837-3

Fluoride, a global environmental pollutant, is ubiquitous in aquatic environments and coexists with selenium, which can cause complex effects on exposed organisms. However, data on the interaction of fluoride and selenium remain scarce. In this study, female zebrafish (Danio rerio) were exposed to fluoride (80 mg/L sodium fluoride) and/or dietary selenomethionine (Se-Met) for 30, 60 and 90 days, the effects on the liver of zebrafish were investigated. The results indicated that an increase in fluoride burden, inhibited growth and impaired liver morphology were recorded after fluoride exposure. Furthermore, fluoride alone caused oxidative stress and inflammation in the liver, as reflected by the increase in ROS and MDA contents, the reduction of anti-oxidative enzymes, the altered immune related enzymes (ACP, AKP, LZM and MPO) and the expression of IL-6, IL-1B, TNF-a, IL-10 and TGF-B. In contrast, co-exposure to fluoride and Se-Met decreased fluoride burden and restored growth. Furthermore, dietary Se-Met alleviated oxidative stress, inflammation and impaired morphology in liver trigger by fluoride. However, dietary Se-Met alone increased the activities of SOD and CAT. These results demonstrate that the protective effect of dietary Se-Met against chronic fluoride toxicity at a certain level.

Data Availability

The datasets used in the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the Shanxi Provincial Central Leading Local Science and Technology Development Fund Project (YDZJSX20231A043), the Shanxi Scholarship Council of China (2020–061), the Shanxi Provincial Key Research and Development Project (201903D221009), the earmarked fund for Modern Agro-industry Technology Research System (2022HX027), and the National Natural Science Foundation of China (31502141; 31440087).

Author information

Authors and Affiliations

  1. College of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, China

    Xiulin Zhang & Jianjie Chen

  2. College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, 453003, Henan, China

    Xiulin Zhang, Xiaobing Wei & Runxiao Zhang

  3. College of Food Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi, China

    Jinling Cao

  4. School of Biology and Food Engineering, Anyang Institute of Technology, Anyang, 455000, Henan, China

    Guodong Wang

  5. College of Food and Environment, Jinzhong College of Information, Taigu, 030801, Shanxi, China

    Lijuan Li

Contributions

X.Z.: data curation, formal analysis, writing – original draft, writing – review & editing, conceptualization. X.W.: review, formal analysis. J.C.: review, formal analysis. G.W.: supervision, methodology. R.Z.: review. L.L.: review. J.C.: writing – review & editing, validation, supervision, project administration.

Corresponding authors

Correspondence to Jinling Cao or Jianjie Chen.

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Competing interests

The authors declare no competing interests.

Conflict of Interest

The authors declare no competing interests.

Supplementary Information

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