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[Damage and changes in expression of autophagy related factors LC3 and p62 of condylar cartilage in fluorosis mouse]Abstract
Purpose: To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.
Methods: Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type ? collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.
Results: Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type ? collagen and p62 decreased in the experimental group.
Conclusions: There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.
Original abstract online at https://pubmed.ncbi.nlm.nih.gov/39005084/