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Fluoride and Biological Calcification I: Effect of Fluoride on Collagen-Induced In Vitro Mineralization and Demineralization Reactions.
| Biological Trace Element Research | Monica Kakkar, Vivek Kapoor, S. K. Singla & R. K. Jethi | [Epub ahead of print]
Bone Mineralization/Quality Bones/Joints Collagen Animal Study
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Abstract

An in vitro system employing collagen isolated from the sheep tendons to induce mineralization and demineralization reactions was used not only to study the effect of various concentrations of fluoride on the collagen-induced mineralization and demineralization reactions but also to compare their action with the inhibitors of mineralization and/or demineralization. Studies demonstrated that under physiological conditions, at lower concentrations (5 × 10-6 to 5 × 10-5 M) fluoride inhibited while at higher concentrations (> 10-4 M), it stimulated the collagen-induced in vitro mineralization. At higher concentrations, fluoride was also found to inhibit the demineralization of the collagen bound preformed mineral phase. At low concentrations, fluoride acted like Mg2+ to inhibit mineralization while at higher concentration, it acted like crystal poisons (e.g., pyrophosphate phosphonates, citrate) to inhibit demineralization. However, unlike magnesium and pyrophosphate, fluoride at its higher concentrations was found to stimulate rather than inhibit the process of mineralization.

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Acknowledgments

The authors are grateful to Mr. K. K. Maheshwari for standardizing the methodology and initiating the work as a research scholar before leaving for higher studies. The authors are indebted to Departments of Biochemistry at Panjab University Chandigarh in Punjab and Himalayan Institute of Medical Sciences at Dehradun in Uttrakhand, for providing facilities and necessary funds for the studies.

Author information

Affiliations

  1. Department of Biochemistry, NRI Medical College and General Hospital, Mangalagiri Mandal, Guntur District, Chinakakani, Andhra Pradesh, 522503, India

    Monica Kakkar

  2. Dr. Harvansh Singh Judge Institute of Dental Sciences & Hospital, Panjab University, Chandigarh, 160014, India

    Vivek Kapoor

  3. Department of Biochemistry, Panjab University, Chandigarh, 160014, India

    S. K. Singla & R. K. Jethi

Corresponding author

Correspondence to Monica Kakkar.

*Original abstract online at https://link.springer.com/article/10.1007%2Fs12011-020-02340-3