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Investigation of the genotoxic effects of fluoride on a bone tissue model .
| Toxicological Research | Volobaev VP, Kalyuzhnaya EE, Schetnikova EA, Korotkova AD, Naik AA, Bach SN, Prosekov AY, Larionov AV. | 36:337–342.
Genotoxicity Bone Cancer Bone Cells Bones/Joints Cancer DNA effects Genotoxicity Mechanisms of Cellular Toxicity Cell Study
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Abstract

Fluorides are thought to be a major cause of osteocarcinogenesis, due to their widespread industrial use, ability to accumulate in bone tissue, and genotoxic and probable carcinogenic properties. In vitro experiments investigating the genotoxic potential of fluorides in bone tissue models can provide valuable indirect information on their involvement in osteocarcinogenesis. Here, we investigated whether sodium fluoride (NaF) has the ability to induce DNA damage and chromosomal abnormalities in human osteosarcoma cells after 48 and 72 h of exposure. The cell cultures were treated with NaF in concentrations of 0, 20, 100 and 200 ?g/ml. The level of DNA damage was assessed by the comet assay, and the frequency of chromosomal abnormalities by a micronucleus test. A significant increase in DNA damage indicators was noted in the samples treated with fluoride concentrations of 100 and 200 µg/ml, after 48 and 72 h of exposure. The micronucleus test revealed a dose-dependent increase in cells with micronuclei, nucleoplasmic bridges and nuclear protrusions. Increasing the concentration of NaF led to an increase in the prevalence of cytogenetic indicators after both treatment durations. This demonstrated ability of fluorine to exert genotoxic effects on bone cells indirectly indicates the possible importance of fluoride in the aetiology of osteosarcoma.

*Original abstract online at https://link.springer.com/article/10.1007/s43188-020-00039-0

 

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Acknowledgements

The study was conducted with financial support from the Russian Foundation for Basic Research and the administration of the region of Kemerovo (No 18-44-420012 p_a, agreement with AKO No 8, 28 June 2018), and from a stipend for young scientists from the President of the Russian Federation.

Author information

Affiliations

  1. Department of Genetics, Kemerovo State University, Krasnaya St 6, Kemerovo, Russia, 650000

    V. P. Volobaev, E. E. Kalyuzhnaya, E. A. Schetnikova, A. D. Korotkova, A. A. Naik, S. N. Bach, A. Y. Prosekov & A. V. Larionov

  2. Tomsk State University, Lenin Avenue, 36, Tomsk, Russia, 634050

    E. S. Serdyukova

Corresponding author

Correspondence to V. P. Volobaev.

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Conflict of interest

The authors declare that they have no conflict of interest.

*Original abstract online at https://link.springer.com/article/10.1007/s43188-020-00039-0