4. Discussion
... the numbers of Nissl bodies in neurons in the hippocampus and cortex of
brains from both adult rats and their pups with fluorosis were lowered.
Such reduction in Nissl bodies, showing large granular bodies consisting
of rough endoplasmic reticulum with rosettes of free ribosomes
carrying out protein synthesis, indicates injury of neurons [23].
In cerebral cortex of the rats, treated with 100 ppm of sodium fluoride, there was disorganization of granular cell layer. The granule cells were accumulated in the form of clumps. Some pyramidal neurons showed chromatolysis (Fig. 2) and were shrunken with vacuolation around them (Fig. 4). The nuclei of some pyramidal neurons were eccentric in neuroplasm and others were showing necrosis. Some granule cells were swollen in shape and size (Fig. 5).
... In rats treated with 200 ppm of sodium flu
In conclusion, the effects of NaF on a cell’s survival and integrity
suggested that different concentrations NaF had direct toxic effects
on the Neuro-2A cell line. NaF not only reduced the expression of
select axonal proteins, but also prevented the secretion and delivery
of the neurotransmitter glutamate.We found that expression
of glutamate and its receptor, NMDAR, decreased with increasing
dosages of NaF. Presumably, NaF can influence the generation and
functions of axons, and limit
from 4. Discussion
... In order to gain a better understanding of the NaF neurotoxicity and its underlying mechanisms, the NaF-treated rats and SH-SY5Y cells were used in the present study. The MWM [Morris water maze test] results showed that exposure of rats to NaF is associated with decreased learning and memory abilities. This is consistent with previous studies where animals exposed to fluoride with similar dosages and duration can also demonstrated different characteristics of neurotoxic
