Grading and quantification of dental fluorosis in zebrafish larva.
October 15, 2016
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Archives of Oral Biology
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Zhang Y, Zhang Y, Zheng X, Xu R, He H, Duan X.
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70:16-23.
Section snippets
Zebrafish culture and fluoride treatment
The adult AB zebrafish were maintained at the zebrafish facility at 28 °C with a cycle of 14 h light and 10 h dark, and they were feed with brine shrimp three times a day. One male and one female zebrafish were paired for the spawning. All the animal experiment protocols were approved by IRB of School of Stomatology, the Fourth Military Medical University.
Once the zebrafish eggs were laid, they were cultured in the embryo me