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Community water fluoridation predicts increase in age-adjusted incidence and prevalence of diabetes in 22 states from 2005 and 2010

Abstract Community water fluoridation is considered a significant public health achievement of the 20th century. In this paper, the hypothesis that added water fluoridation has contributed to diabetes incidence and prevalence in the United States was investigated. Panel data from publicly available sources were used with population-averaged models to test the associations of added and natural fluoride on the outcomes at the county level in 22 states for the years 2005 and 2010

Grading and quantification of dental fluorosis in zebrafish larva.

Section snippets Zebrafish culture and fluoride treatment The adult AB zebrafish were maintained at the zebrafish facility at 28 °C with a cycle of 14 h light and 10 h dark, and they were feed with brine shrimp three times a day. One male and one female zebrafish were paired for the spawning. All the animal experiment protocols were approved by IRB of School of Stomatology, the Fourth Military Medical University. Once the zebrafish eggs were laid, they were cultured in the embryo me