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Effect of fluoride on expression of pura gene and CaM gene in newborn rat osteoblasts.
| Fluoride | Tang Q, Yang X, Tang G, Wang Y, Yu Y. | 40(1):31-36.
Apoptosis Bone Cells Bones/Joints DNA effects Mechanisms of Cellular Toxicity Osteoblasts Cell Study
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Abstract

To explore the effect of fluoride (F) on the expression of purine-rich element-binding protein (PURA) gene and calmodulin (CaM) gene in osteoblasts of newborn rats, parietal calvaria bone osteoblast cultures of 48-hr-old rats were treated for 48 hr with sodium fluoride (NaF) at concentrations of 0 (control), 0.5, 2, and 8 mg/ L. The expression of PURA gene and CaM gene was determined by reverse transcription polymerase chain reaction (R T-PCR). The results indicated that F significantly enhanced (p<0.05 to p<0.01) the expression levels of the two genes in the osteoblast cultures with increasing F concentrations compared with the control group