According to the World Health Organization (WHO) reports, Iran is located in the global fluoride belts, so that is why carrying out extended research on this contaminant anion in water supplies must be considered. Due to the various industries in the Ardakan city, this region is severely suffering from fluoride contamination. This study was designed to investigate the fluoride concentration and its distribution pattern as well as related health risk assessment in groundwater resources of 28 villages of the Ardakan city in Yazd province using SPADNS method according to standard instructions. Our results show that, the average concentration of fluoride was 2.92?mg/l (range: 0.9–6?mg/l), also in half of the villages, the concentration range of this element was over than standard level (1.5?mg/l) given by WHO rules. In addition, risk assessment results showed that HQ value is higher than 1 in 46.4% of samples of groundwater resources in age groups of infants, children, teenagers and adults. Therefore, it is necessary to take measures to reduce fluoride concentration in drinking water in order to control resultant fluorosis. Actions should be implemented to enhance monitoring of fluoride levels to avoid the potential risk of high Fluoride concentration.
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