Abstract

The present study was undertaken to investigate the toxic effects of sodium fluoride (NaF) on sperm motility and abnormality. Treatment of epididymal sperm suspensions with different doses of NaF (0.1, 1, 10, and 100 mg/ml) significantly reduced sperm motility at 15- and 30-min time intervals. Further, incubation at the same concentrations of NaF for 5-, 10-, and 15-min time intervals decreased sperm motility in a dose- and time-dependent manner. The present investigation revealed for the first time the adverse effect of lower doses of NaF (0.1 mg/ml) on sperm motility. Further, higher doses of NaF (10 and 100 mg/ml) caused a complete loss of sperm motility with a concomitant increase in sperm abnormality, which reflected the toxic effect of NaF on spermatozoa and its capability in fertilization.

• Related Studies :

  • Microdose vasal injection of sodium fluoride in the rat

    A single microdose (50 micrograms/50 microL) injection of sodium fluoride (NaF) into the vasa deferentia of adult male albino rats (Rattus norvegicus) caused arrest of spermatogenesis and absence of spermatozoa in the lumina of the seminiferous tubules of the testes, which consequently led to a decline in the sperm count

  • Reversible fluoride induced fertility impairment in male mice

    Sodium fluoride (NaF) fed to adult male albino mice at a dose of 10 mg and 20 mg/kg body weight, caused a significant decrease in sperm county and motility. Scanning electron microscopy and silver nitrte staining showed large numbers of deflagellated spermatozoa, with acrosomal, midpiece and tail abnormalities. The treatment

  • Testing the potential of sodium fluoride to affect spermatogenesis in the rat

    The potential of sodium fluoride (NaF) to affect spermatogenesis and endocrine function was assessed in P and F1 generation male rats. Male and female experimental rats received sodium fluoride in their drinking water at one of four concentrations (25, 100, 175, 250 ppm). P generation male and female rats were

  • Amelioration of fluoride toxicity in some accessory reproductive glands and spermatozoa of rat

    Sodium fluoride (NaF) at a dose of 10 mg/kg body weight was administered orally to male rats (Rettus norvegicus) daily for 30 and 50 days to evaluate the effect of the physiology of some sex accessory glands and sperm functions. The effects of withdrawal upon cessation of NaF ingestion, and

  • Fluoride interferes with sperm fertilizing ability via downregulated SPAM1, ACR and PRSS21 expression in rat epididymis.

    Fluoride is a widespread environmental pollutant which can induce low sperm quality and fertilizing ability, however, the underlying mechanism is still remaining unclear. Hence, we aimed to investigate the influence of fluoride on sperm fertilizing ability via some key proteins in epididymis. For this, forty adult rats were assigned randomly

• Related FAN Content :

  • Fluoride's Effect on the Male Reproductive System -- In Vitro Studies

    Carefully controlled in vitro studies have found that direct exposure of fluoride to the testes or semen inhibits testosterone production and damages sperm. While researchers have known since the 1930s that mega concentrations of fluoride can completely (but reversibly) immobilize sperm, it was not until the 1970s and 1980s that researchers found that relatively modest concentrations of fluoride could cause damage prior to complete immobilization.

  • Fluoride's Effect on Male Reproductive System -- The "Sprando/Collins" Anomaly

    In contrast to the findings of over 60 animal studies from other research teams, a series of studies by FDA researchers Sprando & Collins reported virtually no evidence of reproductive toxicity among animals treated with very high levels of fluoride exposure. The reasons for this discrepancy remains unclear. Excerpts from Sprando/Collins' Studies: "This study

  • Fluoride's Effect on Male Reproductive System: Animal Studies

    Over 60 studies on animals (including rats, mice, roosters, and rabbits) have found that fluoride adversely impacts the male reproductive system. These studies have repeatedly found the following effects: (1) decreases in testosterone levels; (2) reduced sperm motility; (3) altered sperm morphology; (4) reduced sperm quantity; (5) increased oxidative stress; (6) and reduced capacity to breed.

  • Fluoride's Effect on Male Reproductive System - Human Studies

    Consistent with in vitro and animal research, studies of human populations have reported associations between fluoride exposure and damage to the male reproductive system. Most notably, a scientist at the Food & Drug Administration reported in 1994 that populations in the United States with more than 3 ppm fluoride in their water had lower "total fertility rates" than populations with lower fluoride levels.