Abstract

Fluoride (F-) compounds are present on the earth’s surface, water, volcanoes and are also a product of petrochemical and cement industries. Little amounts of F- are required for the formation of bones and enamel, however, according to World Health Organization (WHO), ingestion of over 1.5 mg/L of F- may be a health hazard due to the toxic effects on the kidney, liver and it may also cause dental or skeletal fluorosis. The aim of this study is to compare the genotoxic damage in cell of oral epithelium detected by (the) comet assay, through a population of scholars from two communities located in the city of Tula; one consuming high concentration of F- from drinking-water San Miguel Vindhó (SMV) and the other with levels under the limit La Malinche (LaM). 113 (students) teenagers between the ages of 12 to 15 were selected to obtain epithelial cells by internal cheek brushing. 30 of them were selected (from) by the dental clinic service of the Health Science Institute (UAEH, Mexico) as negative and positive control groups before and after professional appliance of sodium fluoride (NaF) (2%). 31 from LaM and 52 from SMV. 200 cells per person were analyzed to measure tail moment, tail length. Visual scoring was used to classify results according to degrees of damage. These results showed a significant difference between the populations, indicating very low basic damage in both the negative control group and LaM and severe damage in both the exposed population and the positive control group. These results indicate that high levels of F- may be the cause of genotoxicity in oral epithelial cells.

• Related Studies :

  • Cytogenetic effect of inorganic fluorine compounds on human and animal cells in vivo and in vitro

    The object of this study was the mutagenic effect of hydrogen fluoride, cryolite and their mixtures on chromosomes of cells of animals subjected to the inhalation of these compounds and also the mutagenic effect of sodium fluoride on cells of human leucocyte cultures. An increase from 1.24 (in the control) to

  • [Dependence of lethality and incidence of chromosome aberrations induced by treatment of synchronized human diploid fibroblasts with sodium fluoride on different periods of the cell cycle].

    The cytotoxic and clastogenic effects of sodium fluoride during various phases of cell cycle of human cultured diploid fibroblasts were examined. The cells in confluence were synchronized at G1/G0 phase by a period of growth in medium containing 1% serum (low serum medium). To obtain the cells in S phase

  • Sodium fluoride induced chromosome aberrations and sister chromatid exchange in cultured human lymphocytes

    Experimental sodium fluoride (NaF) up 10 30 times the level recommended In drinking waler (1 ppm) was compared with an inorganic salt for its ability to induce chromosome aberrations and sister chromatid exchange (SCE) in cultured human lymphocytes. An increase in the frequencies of chromosome aberrations but not of SCE

  • Fluoride in the Central Nervous System and Its Potential Influence on the Development and Invasiveness of Brain Tumours-A Research Hypothesis.

    The purpose of this review is to attempt to outline the potential role of fluoride in the pathogenesis of brain tumours, including glioblastoma (GBM). In this paper, we show for the first time that fluoride can potentially affect the generally accepted signalling pathways implicated in the formation and clinical course

  • Mutagenic effect of hydrogen fluoride on animals

    The mutagenic effect of hydrogen fluoride in concentration 1.0 mg/m-3 was studied in rats and mice. Prolonged inhalation of this compound increased the frequency of cells with chromosome abnormalities in the bone marrow of albino rats. The mutagenic effect was higher in older animals. No dominant-lethal effect was observed in

• Related FAN Content :

  • Fluoride's Mutagenicity: In vivo Studies

    Consistent with dozens of in vitro studies, a number of in vivo studies, in both humans and animals, have found evidence of fluoride-induced genetic damage. In particular, research on humans exposed to high levels of fluoride have found increased levels of "sister chromatid exchange" (SCE). As noted in one study: "In

  • Fluoride's Mutagenicity: The "Oral Health Research Institute's" Studies

    Although many in vitro and in vivo studies have detected mutagenic effects from fluoride exposure, the Oral Health Research Institute at Indiana University's School of Dentistry has repeatedly failed to find any such effect in multiple studies on the subject.

  • Fluoride/Osteosarcoma Link Is Biologically Plausible

    The "biological plausiblility" of a fluoride-osteosarcoma link is widely acknowledged in the scientific literature. The biological plausibility centers around three facts: 1) Bone is the principal site of fluoride accumulation, particularly during the growth spurts of childhood; 2) Fluoride is a mutagen when present at sufficient concentrations, and 3) Fluoride can stimulate the proliferation of osteoblasts (bone-forming cells).

  • NTP Bioassay on Fluoride/Cancer (1990)

    In 1977, the U.S. Congress requested that animal studies be conducted to determine if fluoride can cause cancer. The result of the Congressional request was an extensive animal study conducted in the 1980s by the National Toxicology Program (NTP) and published in 1990. The main finding of NTP's study was a dose-dependent increase in osteosarcoma (bone cancer) among the fluoride-treated male rats. 

  • Micronucleus and Sister Chromatid Exchange Frequency in Endemic Fluorosis

    The rise of sister chromatid exchange (SCE) and micronucleus (MN) in the peripheral blood lymphocytes of the fluorine-intoxicated patients indicates that fluorine is a mutagenic agent which can cause DNA and chromosomal damage.