Abstract

Cytogenetic analysis was carried out on human lymphocytes of 73 fluorosis-affected men from the endemic fluorosis region in Nalgonda district of Andhra Pradesh, India, who were drinking water with a mean concentration of fluoride (F) of 4.13± 0.55 mg/L, range 1.56–8. 36 mg/L. Eighty healthy men, of a similar age living near the endemic fluorosis area,drinking water with <1 mg F/L were selected as controls. The conventional cytogenetic protocols for detecting chromosomal aberrations (CA) and sister chromatid exchanges (SCE) were performed. More CA were present in the subjects from the endemic fluorosis region, 3.57±0.27%, compared to the controls, 0.68± 0.09% (p<0.01). Similarly, more SCE were present in the subjects from the endemic fluorosis region, mean 8.96±0.68 SCE/cell, compared to the controls, mean 3.58 ± 0. 67 SCE/cell (p<0.001). This indicates that F acts as a mutagen inducing chromosomal
damage in fluorosis-affected men.

• Related Studies :

  • Investigation of the genotoxic effects of fluoride on a bone tissue model .

    Fluorides are thought to be a major cause of osteocarcinogenesis, due to their widespread industrial use, ability to accumulate in bone tissue, and genotoxic and probable carcinogenic properties. In vitro experiments investigating the genotoxic potential of fluorides in bone tissue models can provide valuable indirect information on their involvement in

  • Sodium fluoride induced chromosome aberrations and sister chromatid exchange in cultured human lymphocytes

    Experimental sodium fluoride (NaF) up 10 30 times the level recommended In drinking waler (1 ppm) was compared with an inorganic salt for its ability to induce chromosome aberrations and sister chromatid exchange (SCE) in cultured human lymphocytes. An increase in the frequencies of chromosome aberrations but not of SCE

  • DNA damage induced by fluoride in rat kidney cells.

    DNA damage by fluoride to newborn rat kidney cells isolated by enzymic digestion is reported. The cells were exposed for 24 hr to sodium fluoride at NaF concentrations of 0, 0.2, 0.4, 0.8, and 1.0 mM. Damage to DNA was determined by single cell gel electrophoresis assay (Comet assay). Significant breakage of DNA strands

  • Cytogenetic effects of hydrogen fluoride gas on maize

    Maize seedlings of the genotype C I Sh Wx were fumigated with hydrogen fluoride gas (HF) continuously for 4, 6, 8 and 10 days. Miscrospore mitosis of the treated plants indicated the presence of fragments and bridges suggesting the occurrence of the phenomenon of breakage-fusion-bridge cycle of McClintock. This phenomenon

  • Mutagenic effect of hydrogen fluoride on animals

    The mutagenic effect of hydrogen fluoride in concentration 1.0 mg/m-3 was studied in rats and mice. Prolonged inhalation of this compound increased the frequency of cells with chromosome abnormalities in the bone marrow of albino rats. The mutagenic effect was higher in older animals. No dominant-lethal effect was observed in

• Related FAN Content :

  • Fluoride/Osteosarcoma Link Is Biologically Plausible

    The "biological plausiblility" of a fluoride-osteosarcoma link is widely acknowledged in the scientific literature. The biological plausibility centers around three facts: 1) Bone is the principal site of fluoride accumulation, particularly during the growth spurts of childhood; 2) Fluoride is a mutagen when present at sufficient concentrations, and 3) Fluoride can stimulate the proliferation of osteoblasts (bone-forming cells).

  • NTP Bioassay on Fluoride/Cancer (1990)

    In 1977, the U.S. Congress requested that animal studies be conducted to determine if fluoride can cause cancer. The result of the Congressional request was an extensive animal study conducted in the 1980s by the National Toxicology Program (NTP) and published in 1990. The main finding of NTP's study was a dose-dependent increase in osteosarcoma (bone cancer) among the fluoride-treated male rats. 

  • Fluoride & Liver Cancers in NTP Bioassay

    On October 28, 1988, Battelle Columbus Laboratories submitted its Final Report to the NTP concerning the results of the Mouse study. The principal finding of Battelle's report was that a dose-dependent increase of a rare liver cancer (hepatocholangiocarcinoma) had occurred in the fluoride-treated male and female mice.

  • Micronucleus and Sister Chromatid Exchange Frequency in Endemic Fluorosis

    The rise of sister chromatid exchange (SCE) and micronucleus (MN) in the peripheral blood lymphocytes of the fluorine-intoxicated patients indicates that fluorine is a mutagenic agent which can cause DNA and chromosomal damage.

  • A Critique of Gelberg's Study on Fluoride/Osteosarcoma in New York

    The case-control study by Gelberg, published first as a PhD dissertation and then later in two peer-reviewed journals, may represent the most substantive study on fluoride/osteosarcoma previous to Bassin’s 2001 analysis. In assessing Gelberg’s data, we were at first struck by the existence of several notable errors in both the thesis and papers. While these errors do raise questions about the study, our primary concern with Gelberg’s work relates to the methods she used to analyze her data.