Assessment of non skeletal fluorosis in the children (6-14 years) was conducted in the two blocks (a) Palera, Heerawala, Nayabas, Saipur and Birasana of Jamwaramgarh block, (b) Chitanukalan,Jugalpura,Sunderka bas ,Peelwa and Sirsali of Amber of Jaipur district of Rajasthan, India of the children of the study villages. Serum samples of these children showed elevated levels of alkaline phosphatase (ALP), serum glutamic transaminase (SGOT), Serum glutamic pyruvic transaminise (SGPT), and serum Bilirubin. Thus our data shows that High fluoride concentration in serum level affect the liver function. This finding is also associated with impaired liver function as assessed by biochemical parameters. Increased fluoride concentration in drinking water is also responsible for the increased the disease pattern.