Abstract

Thirty-two one-month-old Wistar albino rats were divided randomly into four equal groups of eight (female:male = 3:1). To assess damage to DNA in their brain cells, the first group (1) of rats served as the untreated control, the second group (2) was administered high fluoride (HiF, 100 mg NaF/L in the drinking water), the third group (3) was placed on a low iodine intake (LI, 0.0855 mg I/kg diet), and the fourth group (4) was exposed to the same high fluoride and low iodine combined (HiF+LI). At 20 months of age, the rats were anesthetized and their brain cells prepared for single cell gel electrophoresis (SCGE = comet assay). DNA damage in the brain cells assessed by the tailing ratio percent was 24.68±20.81% in the control group 1 and in the HiF, LI, and HiF+LI groups 2, 3, and 4, it was 90.93±9.17%, 89.04±4.99%, and 92.48±4.04%, respectively. Moreover,the proportion of grade III damage to the brain cells increased by 24.00% in the HiF group 2, 21.88% in the LI group 3, and 33.33% in the HiF+LI group 4, but only by 8.0% in the control group 1. These results indicate that DNA strands in the brain cells of rats are adversely affected by exposure to high fluoride, low iodine, and together in combination.

• Related Studies :

  • [Effects of selenium and zinc on the DNA damage caused by fluoride in pallium neural cells of rats].

    To investigate the effects of fluoride on DNA damage as well as the effects of selenium and zinc against fluoride respectively or jointly in pallium neural cells of rats, single cell gel electrophoresis was used to detect the DNA damage of neural cells prepared in vitro. The results showed that

  • [Effects of high fluoride and low Iodine on learning-memory and TchE of brain in offspring rats].

    Objective To study the effect of high level fluoride and low level iodine on learning-memory in offspring rats and possible mechanism. Methods Thirty-two Wistar rats were randomly divided into four groups each of eight (female:male=(3:1).) The rats were treated with high fluoride (100 and 150 mg NaF/L), low iodine (0.0855 mg/kg),

  • Effects of fluoride on DNA damage, S-phase cell-cycle arrest and the expression of NF-kappaB in primary cultured rat hippocampal neurons.

    The mechanisms underlying the neurotoxicity of fluorosis still remain obscure. To investigate DNA damage, cell-cycle distribution and expression of nuclear factor kappa B (NF-kappaB) induced by fluoride, the primary rat hippocampal neurons were incubated with various concentrations (20mg/l, 40 mg/l, and 80 mg/l) of sodium fluoride for 24 h in

  • [Influence of combined iodine and fluoride on phospholipid and fatty acid composition in brain cells of rats].

    OBJECTIVE:  Investigating the influence of combined iodine and fluoride on phospholipid and fatty acid composition in brain cells of rats. METHODS:  Five groups of rats were provided with deionized drinking water containing 0 and 150 mg/L NaF, and containing both 150 mg/L NaF and 0.003, 0.03 or 3 mg/L KI respectively

  • Apoptosis in brain cells of offspring rats exposed to high fluoride and low iodine.

    To assess brain cell apoptosis induced by high fluoride and/or low iodine in their offspring, 32 one-month old Wistar albino rats were divided randomly into four equal groups, each with six females and two males. The first group of rats served as the untreated controls; the second group received high

• Related FAN Content :

  • Fluoride & Oxidative Stress

    A vast body of research demonstrates that fluoride exposure increases oxidative stress. Based on this research, it is believed that fluoride-induced oxidative stress is a key mechanism underlying the various toxic effects associated with fluoride exposure. It is also well established that fluoride's toxic effects can be ameliorated by exposure

  • Fluoride Exposure Increases Metabolic Requirement for Calcium & Vitamin D

    It is well known that individuals with nutrient deficiencies are more susceptible to fluoride toxicity, including fluoride's bone effects. As discussed in the following studies, fluoride increases the skeleton's need for calcium (and vitamin D) by increasing the amount of unmineralized tissue (osteoid) in the bone. When insufficient calcium and

  • Fluoride: Developmental Neurotoxicity.

    Developmental Neurotoxicity There has been a tremendous amount of research done on the association of exposure to fluoride with developmental neurotoxicity. There are over 60 studies reporting reduced IQ in children and several on the impaired learning/memory in animals. And there are studies which link fluoride to Attention Deficit Hyperactivity Disorder. Teaching

  • NRC (2006): Fluoride's Neurotoxicity and Neurobehavioral Effects

    The NRC's analysis on fluoride and the brain.

  • The Relationship Between Fluoride Exposure & Goitre in South Africa

    As a general rule simple goitre, irrespective of the cause, can be very, or fairly, satisfactorily combated by an adequate increase in man's daily iodine intake, except when the enlargement of the gland is due to the ingestion of excessive amounts of fluorine. The only correct solution to fluorine-induced endemic goitre is the removal of this element from the drinking water.